1. Front Cover.cdr - CORE
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A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S<br />
THE INDUCTION AND GROWTH OF POTATO (SOLANUM TUBEROSUM. L) MICROTUBERS,<br />
SANTE CULTIVAR, RESPONSE TO THE DIFFERENT CONCENTRATIONS OFBAP AND<br />
SUCROSE<br />
Alireza Iranbakhsh 1 , Mostafa Ebadi 2<br />
1 Islamic Azad University, Aliabad Katool, Golestan Province, Iran<br />
2 Islamic Azad University, Damghan Branch, Semnan Province, Iran<br />
E-mail:iranbakhshar@yahoo.com<br />
The effects of different concentrations of Banzil Amino Purin (BAP) and sucrose as induction combinations<br />
on microtuber formation and the time of this process, numbers and fresh and dry weight of microtubers<br />
were investigated. A two-stage culture was used to study the effect of hormonal and sucrose treatments.<br />
In the first stage, a liquid MS medium containing 0.5 mgl -1 BAP + 0.4 mgl -1 GA3 + 20 gl -1 sucrose was used for<br />
the increase of branches. The cultures of single node were grown against white light (4000 - 5000 LUX) and<br />
on sucrose for one month. In the second stage, microtuber formation induced on fluid MS medium<br />
containing different concentrations of sucrose (30, 40, 60, 80 mgl -1 ) and BAP (1, 2, 5, 10 mgl -1 ) was used in<br />
continuous darkness. Microtuber formation was investigated within 10 weeks after induction.<br />
CHARACTERISATION OF SECRETED COMPOUNDS IN NORWAY SPRUCE TISSUE CULTURE<br />
MEDIUM BY CHROMATOGRAPHY AND MASS SPECTROMETRY<br />
Gwe G.Chenyi 1 , S.Chong 2 , S.Koutaniemi 2 , M.Tenkanen 2 , S.C.Fry 3 , A.Kärkönen 1,4<br />
1 Department of Agricultural Sciences, University of Helsinki, Finland<br />
2 Department of Food and Environmental Sciences, University of Helsinki, Finland<br />
3 Institute of Molecular Plant Sciences, University of Edinburgh, United Kingdom<br />
4 MTT Agrifood Research Finland, Jokioinen, Finland<br />
E-mail: gilbert.gwe@helsinki.fi<br />
Oligosaccharides secreted into the culture medium of suspension cultures have been shown to be similar to<br />
those in cell walls [1, 2]. Some secreted oligosaccharides may play a crucial role in plant growth and<br />
development. For example, fungal cell wall-derived oligosaccharides elicit the generation of a defenceassociated<br />
H2O2 burst in extracellular lignin-producing Norway spruce cell cultures [3, 4]. We hypothesise<br />
that soluble oligosaccharides present in the culture medium of spruce cells regulate the constant low levels<br />
of H2O2 present during extracellular lignin formation. Cultured spruce cells were fed with [U-14C]glucose<br />
and treated subsequently with H2O, elicitor, KI or KCl. The culture medium compounds were separated by<br />
paper chromatography and high voltage paper electrophoresis. The results show varying amounts of<br />
certain compounds in different treatments. The positively charged compounds have been further purified<br />
by cation exchange chromatography. The identification (mass spectrometry) of compounds is underway<br />
and their involvement in plant growth and development will be investigated.<br />
References<br />
[1] McNeil et al., Annu. Rev. Biochem. 1984<br />
[2] York et al., Methods Enzymol. 1985<br />
[3] Kärkönen and Fry, J. Exp. Bot. 2006<br />
[4] Kärkönen et al., Planta 2009<br />
POLYPHOSPHATES IN PLANTS<br />
Hélène Pélissier Combescure, Elizabeth Nees Ahm, Tom Hamborg Nielsen<br />
Department of Plant Biology and Biotechnology, Faculty of Life Sciences, Copenhagen University, Copenhagen, Denmark<br />
E-mail: hepel@life.ku.dk<br />
Organisms can store phosphate as polyphosphate, a linear polymer of phosphate residues linked by highenergy<br />
bonds. Polyphosphate serves as an energy reservoir, and chelates heavy metal ions. In bacteria, it<br />
plays a role in stress responses, gene regulation, motility and virulence. The polyphosphate kinase (PPK1),<br />
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