1. Front Cover.cdr - CORE

Recommendations

Info

A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S Photosynthetic responses and tolerances to short- and long-term high-light stress were investigated in seven Arabidopsis ecotypes collected from different environments, including high altitude, high salinity, dry and sunny regions. Plants grown under standard conditions (light intensity 150 µmol m -2 s -1 ) were compared to plants grown at different periods of high light (300-1000 µmol m -2 s -1 ). The different ecotypes showed different degrees of change in morphology, with Col-0 displaying the strongest stress symptoms under high light. Analyses by multicolour fluorescence imaging (blue, green, red and far-red) indicated enhanced stress tolerances in Pf-0, Po-0 and Ws-0, whereas Col-0 and C24 were the most sensitive. Chlorophyll fluorescence kinetics showed that quantum yield is strongly stimulated by high light in Cvi-0 and Nok-2, and nonphotochemical quenching indicates better high-light adaptation in Cvi-0 and C24. Photosynthetic performances in the different ecotypes were also evaluated by gas exchange analysis, and photosynthetic pigments and anthocyanins determined by spectrophotometry. Further work is underway to resolve the mechanisms of stress tolerance in Arabidopsis by comparative proteomics as well as metabolic and transcriptional analyses. Acknowledgements This work was supported by grants MCINN-FEDER/AGL2008-00214 and CVI 03475 (Junta de Andalucía). PEROXISOME-MEDIATED MECHANISMS OF ABIOTIC STRESS TOLERANCE Gopal Chowdhary 1, 2 , Sigrun Reumann 2 1 KIIT School of Biotechnology, Campus XI, KIIT University, Bhubaneswar, Orissa, India 2 Centre for Organelle Research, University of Stavanger, Stavanger, Norway E-mail: gopal.chowdhary@uis.no Present alterations in environmental scenario have led to a serious concern about the loss of plant productivity due to extreme abiotic stress conditions, which mainly include drought, salt, metal toxicity high and low temperature. The tremendous amount of losses caused a thrust on high end research to make plants tolerant to these stress conditions. Many plant species evolved effective, endogenous mechanisms to cope with abiotic stress conditions. Our group focuses of stress adaptation mechanisms in the model plant Arabidopsis that involve peroxisome functions. Over the past years, our lab has established the Arabidopsis peroxisome proteome database. Novel plant peroxisomal proteins have been identified on a large scale by experimental proteome analyses and bioinformatics-based protein prediction models. These proteins of largely unknown function have been subjected to a 2nd order screen for genes that are highly inducible by abiotic stress conditions in microarray-based transcriptome studies using the Genevestigator database. Selected candidate genes were subcloned and validated as peroxisome targeted by in vivo subcellular targeting analysis. Many proteins have been validated as peroxisomal, including, for instance, a calcium binding annexin homolog, an alanine:glyoxylate aminotransferase homolog, and a polyamine oxidase. Stress-inducible gene expression is presently being validated by real-time PCR. The predicted functions and mechanisms underlying abiotic stress resistance are being predicted by bioinformatics tools such as co-expression analysis and experimentally investigated by loss- and gain-of-function mutants. To translate the knowledge from model Arabidopsis to important crop plants, Oryza orthologs are being analyzed in parallel. THE EFFECT OF DIFFERENT RADIATION CONDITIONS ON THE QUALITATIVE AND QUANTITATIVE COMPOSITION OF UNBOUND PHENOLIC COMPOUNDS IN BARLEY LEAVES EXAMINED BY UV-VIS ABSORPTION SPECTROPHOTOMETRY AND HPLC-DAD. Jakub Nezval 1 , Jiří Kalina 2 , Michal štroch 1 , Vladimír špunda 1 1 Department of Physics, Faculty of Science, University of Ostrava, Ostrava, Czech Republic 2 Department of Chemistry, Faculty of Science, University of Ostrava, Ostrava, Czech Republic E-mail: nezval.jakub@gmail.com In this work we focused on the role of phenolic compounds (PC) in the plant protection against deleterious effects of the ultraviolet radiation (UV-R). These compounds are able to absorb UV-R before it can reach sensitive biomolecules (UV-shielding mechanism) or they can inactivate harmful reactive compounds, which are produced in cells by UV-R. We tried to elucidate interaction between UV-R and photosynthetic active 75 X X I V S P P S C O N G R E S S 2 0 1 1
A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S radiation (PAR) in the induction of PC accumulation. As a model plant we used spring barley (Hordeum vulgare L. cv. Bonus) cultivated in 4 different radiation conditions. Two groups of plants were cultivated in the absence of UV-R under the high (1000 µmol m -2 s -1 ) and low intensities (50 µmol m -2 s -1 ) of PAR. Other two groups were cultivated in aforesaid PAR conditions, but they were treated by higher doses of UV-B (280-320 nm). Important part of this work is the standardization of spectrophotometric and HPLC-DAD methods, which allow us to determine the qualitative and quantitative composition of unbound leaf PC. We made summarization of obtained parameters (absorption spectra, retention times, calibration equations). The results of the HPLC analysis of leaf extracts show that response of specific PC to various levels of UV-B and PAR differs significantly. EFFECT OF DIFFERENT ANION CHANNEL INHIBITORS ON THE GERMINATION OF THE 7B- 1, A TOMATO (SOLANUM LYCOPERSICUM L.)MUTANT DEFECTIVE IN BL SIGNALING. Jan Humplík 1,2 , Jana Balarynová 2 , Veronique Bergougnoux 2 , Martin Fellner 2 1 Centre of the Region Haná for Biotechnological and Agricultural Research Department of Growth Regulators, Palacky University, Olomouc, Czech Republic 2 Palacky University and Institute of Experimental Botany, Academy of Science of the Czech Republic, Olomouc, Czech Republic E-mail: jfhumplik@gmail.com The tomato mutant 7B-1 was previously described as being more tolerant than corresponding wild-type (WT) to various abiotic stresses in a blue-light dependent manner. In order to understand the role of light during germination, 7B-1 and WT seeds germinated on the basal MS medium in different light conditions. Under continuous blue-light (BL), mutant seeds germinated better than seeds of the WT. The involvement of anion channels in germination was assessed by adding anion channel blockers 9-AC, niflumic acid or DIDS to the culture medium. Under BL, niflumic acid strongly inhibited germination of 7B-1 seeds whereas germination of WT seeds was less affected. Inhibitors 9-AC and DIDS had no essential effect on germination of 7B-1 seeds. Differently in WT, 9-AC inhibited seed germination additionally to BL, whereas DIDS abolished the inhibitory effect of BL. Preliminary data from expression analysis of ESTs homologous to selected Arabidopsis channels suggest that during seed germination under BL, their expression is affected by the 7B- 1 mutation. Our results suggest an important role of anion channels and 7B-1 gene in the process of imbibition/germination under BL. Acknowledgements The work was supported by Ministry of Education of the Czech Republic (grants no. ME10020, MSM6198959215). CPD PHOTOLYASE REPAIRS ULTRAVIOLET-B-INDUCED CPDS IN RICE CHLOROPLAST AND MITOCHONDRIAL DNA Masaaki Takahashi, Mika Teranishi, Hiroyuki Ishida, Junji Kawasaki, Atsuko Takeuchi, Jun Hidema Graduate School of Life Sciences, Tohoku University, Sendai, Japan E-mail: j-hidema@ige.tohoku.ac.jp Ultraviolet-B (UVB) (280 to 320 nm) radiation in sunlight suppresses photosynthesis and protein biosynthesis, thereby decreasing growth and productivity. A principal cause of such UVB-induced growth inhibition in plants is the major UVB-induced DNA lesion, cyclobutane pyrimidine dimers (CPDs). UVB radiation induces the formation of CPDs in nuclear, chloroplast, and mitochondrial DNA. Repair of CPDs is therefore essential for plant survival while exposed to UVB-containing sunlight. Nuclear repair of the UVBinduced CPDs by CPD photolyase is well known, but repair processes in plant chloroplasts and mitochondria are not understood. Here, we report the photoreactivation of CPDs in chloroplast and mitochondrial DNA in rice. Biochemical and subcellular localization analyses using rice strains with different levels of CPD photolyase activity and transgenic rice strains showed that full-length CPD photolyase is encoded by a single gene, not a splice variant, and is expressed and targeted not only to nuclei but also to chloroplasts and mitochondria. Thus, a single DNA repair enzyme CPD photolyase is “triple targeted” in rice cells. The results indicate that rice may have evolved a CPD photolyase that functions in all three organelles that contain DNA to protect cells from the harmful effects of UVB radiation. 76 X X I V S P P S C O N G R E S S 2 0 1 1
Page 1 and 2:
��
Page 3 and 4:
Foreword eword ord A B S T R A C T
Page 5 and 6:
Program of the Congress gram of the
Page 7 and 8:
A B S T R A C T B O O K - P R O G R
Page 9 and 10:
A B S T R A C T B O O K - P R O G R
Page 11 and 12:
Plenary session 1 A B S T R A C T B
Page 13 and 14:
Plenary session 2 THE BIOLOGY OF AL
Page 15 and 16:
Session 1. Biotechnology and synthe
Page 17 and 18:
Session 1. Biotechnology and synthe
Page 19 and 20:
Session 2. Systems biology and -omi
Page 21 and 22:
Session 2. Systems biology and -omi
Page 23 and 24:
A B S T R A C T B O O K - A B S T R
Page 25 and 26: A B S T R A C T B O O K - A B S T R
Page 27 and 28: Pleanary session 4 A B S T R A C T
Page 29 and 30: Invited talk 4 A B S T R A C T B O
Page 31 and 32: Session 5.Cell biology A B S T R A
Page 33 and 34: Session 6.Organelle biology A B S T
Page 35 and 36: Session 6.Organelle biology A B S T
Page 37 and 38: Session 7.Development A B S T R A C
Page 39 and 40: Session 8.Signalling A B S T R A C
Page 41 and 42: Pleanary session 6 A B S T R A C T
Page 51 and 52: Session 13.Pathogen defense and pla
Page 53 and 54: Session 13.Pathogen defense and pla
Page 67 and 68: Sapporo Medical University, Sapporo
Page 75: A B S T R A C T B O O K - A B S T R
Page 91 and 92: SCF = A B S T R A C T B O O K - A B
Page 93 and 94: A B S T R A C T B O O K - L I S T O
Page 95 and 96: A B S T R A C T B O O K - L I S T O
Page 97 and 98: Sponsors onsors ors A B S T R A C T
show all

1. Front Cover.cdr - CORE

Create successful ePaper yourself

Delete template?

Save as template?