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A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S Sabine Kunz, Edouard Pesquet, Leszek A. Kleczkowski Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, Sweden E-mail: sabine.kunz@plantphys.umu.se The ability of a plant to cope with different environmental factors depends on its energy state. In plants, the energy available for each cell underlies a continuous fluctuation, which is influenced by carbon assimilation and respiration. The response of a plant to available carbohydrates (CH), the main source of energy, is partly coordinated through cell signaling by specific changes in gene expression. These are determined by the interaction of transcription factors (TF) with cis-regulatory DNA elements in promoters of responsive genes. Here we describe experimental approaches to verify the functionality of the three insilico predicted cis-elements SUC/ROS 3, 5 and 6, which are likely to be involved in sucrose-dependent cellsignaling. The use of A. thaliana cell cultures, grown on xylose as carbon source, allows us to distinguish the response of the cells to different CH signals. This set-up, applied to cells transformed with promoterreporter gene constructs that bear the cis-elements in both the forward and reverse orientation in a native as well as synthetic environment, is a valuable tool to determine the involvement of these cis-elements in the different transcriptional responses in the adaptation to changes of available CH. ENDOGENOUS REACTIVE OXYGEN SPECIES (ROS) STATUS AND CELL DEATH IN NITRIC OXIDE (NO) MUTANTS UNDER COPPER EXCESS Zsuzsanna Kolbert, Andrea Pető, Nóra Lehotai, László Erdei Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, Hungary E-mail: kolzsu@bio.u-szeged.hu Enhanced ROS production is associated with abiotic stresses, and NO as a general plant signal plays a role in cell death induction. The possible relationship between ROS and NO was investigated in copper-treated wild type (WT), NO-overproducer (nox1) and -deficient mutant (nia1nia2, nia1nia2noa1-2) Arabidopsis by microscopic methods. Seven-day-old plants were grown in MS medium supplemented with 0, 5, 25 and 50 µM CuSO4. In the elongation zone (EZ) of wild type roots the levels of NO and superoxide anion (O2 .- ) decreased, peroxynitrite (ONOO - ) enhanced as the effect of Cu 2+ exposure, which suggests the reaction between NO and O2 .- to produce ONOO - . The level of ROS was also increased by Cu 2+ , which can be partly responsible for oxidative cell death. In roots of NO-overproducer and -deficient plants the copper-induced alterations in the ROS metabolism and the degree of cell death was more pronounced compared to WT. Based on our results it can be stated, that under Cu 2+ excess NO acts as an antioxidant by eliminating O2 .- and yielding the less toxic ONOO - . The copper-induced cell death is strictly regulated by endogenous NO status: NO content in the root being over or under the optimal level intensifies cell death process. PLASMA MEMBRANE AQUAPORIN PIP2;3 IS RELATED TO HEAT STRESS RESPONSE IN ARABIDOPSIS THALIANA Ayako Tsuchihira 1 , Yuko Hanba 2 , Masayoshi Maeshima 1 1 Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan 2 Kyoto Institute of Technology, Kyoto, Japan E-mail: tsuchihira.ayako@f.mbox.nagoya-u.ac.jp Plants have a large protein family of aquaporin, which facilitate membrane transport of water and other small molecules. Arabidopsis thaliana contains 35 aquaporins divided into four subfamilies: PIP, TIP, NIP and SIP. Plasma membrane aquaporins (PIPs) play a key role of intercellular water transport. PIPs have been reported to response to abiotic stresses. We focused on the physiological function of PIPs under high temperature conditions. Expression of 13 PIP genes was quantified by qRT-PCR in A. thaliana. Among them, only PIP2;3 transcript level was transiently increased when the temperature raised to 36°C . The increased level of mRNA was rapidly decreased within 2 hr even at high temperatures. PIP2;3 promoter-GUS fusions revealed the tissuespecific expression under high temperature. After heat stress, PIP2;3 was predominantly expressed in the 73 X X I V S P P S C O N G R E S S 2 0 1 1
A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S veins of leaves and the vascular tissue of roots. We estimate that this transient alteration of PIP2;3 is tightly related to initial response of heat stress and the increased PIP2;3 functions as water channel in order to regulate the water conditions in plants at high temperature. THE LIGHT-DEPENDENT INDUCTION OF LEAF SENESCENCE Bastiaan Brouwer, Olivier Keech, Agnieszka Ziolkowska, Per Gardeström Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, Sweden E-mail: bastiaan.brouwer@plantphys.umu.se Often, leaves have to deal with reduced light-conditions, generally resulting from shading by neighboring plants. As a common response, they can relocate nutrients from shaded to developing leaves through a process called leaf senescence. Shade- and dark-induced leaf senescence are thought to be initiated by either the relative increase of far-red light on phytochromes or the light dropping below the light compensation point. However, the regulatory mechanisms by which these factors induce leaf senescence are still unclear. We investigated this matter by shading individual attached leaves and analyzing the resulting processes by physiological measurements, transmission electron microscopy and the expression of senescenceassociated genes. The results showed that mild shading led to photosynthetic acclimation within the shaded leaf, whereas strong shading led to leaf senescence when the light intensity was lower than the acclimated light compensation point. Furthermore, using phytochrome null-mutants, we showed that despite an increase in chlorophyll-degradation upon shading, these mutants did not increase the expression of senescence-associated genes. Altogether, our results suggest that the induction of shade-induced leaf senescence mainly depends on the efficiency of carbon fixation rather than on a phytochrome-dependent signaling pathway. OZONE SENSITIVITY OF THE MOST IMPORTANT INDIAN CROPS AND TREE SPECIES Elina Oksanen, Vivek Pandey, Sari Kontunen-Soppela, Sarita Keski-Saari University of Eastern Finland, Joensuu, Kuopio and Savonlinna, Finland E-mail: Elina.Oksanen@uef.fi Indo-Gangetic Plains (IGP) is the bread-basket of rapidly developing India, where intense agriculture, landuse changes, industrialization, urbanization and population growth lead to increasing emissions of precursors for greenhouse gases, such as ozone. It has indicated that surface ozone levels are much above critial levels at most places in the Indian region. Ozone caused losses in gross primary production in India is expected to be roughly 20-30% by 2100. However, species-level information about ozone sensitivity is very scarce. In this development project we screen the genetic variation of ozone sensitivity of the most important Indian crop and tree species (rice, wheat, pea, mustard, teak) addressing tolerance mechanisms. We utilize different and complementary methodological approaches: laboratory experiments in Finland and free-air approaches and natural field sites (low- and high-ozone regions) in IGP area in India. The plants will be studied e.g. for growth, gas exchange, ozone uptake, changes in metabolome and protein profiles, and key genes and enzymes. Metabolome assays from grains (e.g. nutrients and amino acid profiles) will be linked to sensitivity screening. The results from plant responses and ozone monitoring data will be integrated for ozone risk assessment, which is useful for agriculture managers and planners, and ultimately farmers. VARIATION IN HIGH-LIGHT STRESS TOLERANCE BETWEEN ARABIDOPSIS ECOTYPES Espen Granum, Maria Luisa Pérez Bueno, Matilde Barón Department of Biochemistry and Molecular and Cellular Biology of Plants, Estación Experimental del Zaidín, CSIC, Granada, Spain E-mail: e.granum@csic.es 74 X X I V S P P S C O N G R E S S 2 0 1 1
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Foreword eword ord A B S T R A C T
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Program of the Congress gram of the
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A B S T R A C T B O O K - P R O G R
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A B S T R A C T B O O K - P R O G R
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Plenary session 1 A B S T R A C T B
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Plenary session 2 THE BIOLOGY OF AL
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Session 1. Biotechnology and synthe
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Session 1. Biotechnology and synthe
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Session 2. Systems biology and -omi
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Session 2. Systems biology and -omi
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Page 27 and 28: Pleanary session 4 A B S T R A C T
Page 29 and 30: Invited talk 4 A B S T R A C T B O
Page 31 and 32: Session 5.Cell biology A B S T R A
Page 33 and 34: Session 6.Organelle biology A B S T
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Page 37 and 38: Session 7.Development A B S T R A C
Page 39 and 40: Session 8.Signalling A B S T R A C
Page 41 and 42: Pleanary session 6 A B S T R A C T
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Page 67 and 68: Sapporo Medical University, Sapporo
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Page 91 and 92: SCF = A B S T R A C T B O O K - A B
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Page 97 and 98: Sponsors onsors ors A B S T R A C T
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