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A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S the normalization of transcript data, usually done by reference genes that show stable expression in the analyzed tissue or treatment. It has been shown that many commonly used reference genes are not stable expressed in several developmental states i.e. seed germination. During seed germination huge transcriptional changes take place, complicating the identification of reference genes. Therefore we carried out a cross-species approach with Arabidopsis thaliana, Lepidium sativum and Brassica napus using seeds as challenging tissue to identify family-wide reference genes for Brassicaceae seed germination and maturation. We used transcriptome data of Lepidium sativum seed tissues and Arabidopsis thaliana whole seeds to select candidate reference genes and verified their stability with qRT-PCR in both species. Further analysis of transcriptome data from Brassica napus and Arabidopsis thaliana seeds during maturation confirmed that the majority of the new reference genes are also stable expressed during seed maturation. Our cross-species Brassicaceae case-study can therefore be used as a guideline to identify reference genes for other families or other demanding tissues and treatments. COMPUTATIONAL ANALYSIS OF GENES INVOLVED IN NITRIC OXIDE, SA AND JA SIGNALING IN RICE UNDER CADMIUM STRESS Indra Singh 1 , Neetu Singh Yadav 1 , Pragat Agrawal 1 , Kavita Shah 2 1 Bioinformatics Division, Mahila Mahavidyalaya, Banaras Hindu University, Varanasi, India 2 Institute of Environment & Sustainable Development, Banaras Hindu University, Varanasi, India E-mail: singhanjanabhu@gmail.com Complete genome availability of rice provides for study of candidate genes in nitric oxide, jasmonic acid and salicylic acid signalling pathway in plants. We examined in silico, the genes involved and the point of convergence of these signalling pathways under cadmium stress in rice. In NO pathway nitrate reductase and nitric oxide synthase are enzymes that catalyze a balanced production of NO in plants under abiotic stress. Retrieval of NR and NOS gene sequence from rice and detection of their homologs, gene ontology studies and homology modelling provided an insight into the protein products and their function in stress signalling pathway in rice. In SA-JA interacting pathways, 13 genes were found to be expressed that either activate (EDS-1, R-gene, CPR-6, PAD-4, EDS-5, SID-2, TGA-2, SFD-1, PR) or inhibit (CPR-5, NPR-1, SNI-1, SSI-2) the effect of abiotic stress in A. thaliana. Out of these, 7 gene sequences were obtained upon similarity search from O. sativa and are yet not annotated. Using various computational parameters and structural data, these genes were annotated and their functions predicted. Results suggest genes, involved in NO, SA and JA pathways are crosslinking in O. sativa having synergistic as well as antagonistic effects in rice under cadmium toxicity. PLANT PEROXISOMAL PARALOGUES OF GLYCOLATE OXIDASE POSSESS DIFFERENT BIOCHEMICAL PROPERTIES AND PHYSIOLOGICAL FUNCTIONS Anke Kuhn, Martin K.M. Engqvist, Nils Jaspert, Veronica G. Maurino Department of Botany, Cologne Biocenter, University of Cologne, Cologne, Germany E-mail: akuhn1@uni-koeln.de During photorespiration glycolate oxidase (GO) catalyses the oxidation of glycolate into equimolar amounts of glyoxylate and H2O2 in the peroxisomes. The Arabidopsis genome contains a family composed of five GO homologs. Two close related genes, GO1 and GO2, are highly expressed in photosynthetic tissues and support photorespiration. A third paralogue, GO3, showed lower expression in autotroph tissues and the two more distant paralogues showed high homology to human long- and medium-chain 2-hydroxyacid oxidases (HAOX) and are thus called HAOX1 and 2. In silico analysis of differing active site residues suggested different substrate specificities for the different homologues. In this work, the five Arabidopsis GO homologues were expressed as recombinant protein and characterized at the biochemical level. The results obtained together with the isolation and characterization of knock-out mutants allowed to propose that GOX3 would function as a lactate oxidase during recovery of hypoxia and that HAOX 1 and -2 would have a role principally during seed development. 57 X X I V S P P S C O N G R E S S 2 0 1 1
A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S CYTOKININS IN FRESHWATER AND SOIL CYANOBACTERIA AND ALGAE Eva Žižková 1 , Lenka Záveská Drábková 2 , Petre Dobrev 1 , Pavel Přibyl 3 , Silvia Gajdošová 1 , Klára Hoyerová 1 , Miroslav Kamínek 1 , Václav Motyka 1 1 Institute of Experimental Botany AS CR, Prague, Czech Republic 2 Institute of Botany AS CR, Průhonice, Czech Republic 3 Institute of Botany AS CR, Třeboň, Czech Republic E-mail: zizkovae@ueb.cas.cz Cytokinins (CKs) are plant hormones regulating many aspects of growth and development. A great diversity of CK derivatives was identified in vascular plants while only some of them occur in evolutionary older nonvascular organisms. With the aim to contribute to the present knowledge of CK distribution throughout the plant kingdom, endogenous CK profiles were analysed in fifteen freshwater and soil species of Cyanobacteria, Chlorophyta and Chromophyta. In general, isopentenyladenine(iP)-type CKs prevailed over those of trans-zeatin (Z), cis-zeatin (cisZ) and dihydrozeatin (DHZ) in most taxa. Interestingly, in Cyanobacteria (Chroococcales, Nostocales and Oscillatoriales) methylthio-derivatives of iP represented the highest proportion of CKs. None or only trace amounts of CK-N- and O-glucosides were detected in all tested species in contrast to the land plants. Another general trait found for most of analysed species was a higher concentration of cisZ than Z. It is therefore possible that functions of CK-N-glucosides (i.e. deactivation or lowering of biological activity) in cyanobacteria and algae are, at least partially, substituted by cisZ, which is prevailing and less active form compared to Z. Absence of cisZRMP suggest an existence of a cisZ biosynthetic pathway different from that of Z. Acknowledgements Supported by the Czech Science Foundation project P506/11/0774. CHLOROPHYLL FLUORESCENCE AND CHANGES IN STARCH CONTENT IN ROOTSTOCK OF ELEPIDOTE RHODODENDRON CULTIVAR 'CUNNINGHAM'S WHITE' CUTTING GRAFTS DURING PROPAGATION K. Dokane, L. Mertena, D. Megre, U. Kondratovics University of Latvia, Riga, Latvia E-mail: kristine.dokane@gmail.com The cutting-grafting technique is based on grafting scions onto unrooted rootstocks. This method is comparatively quick because during the propagation two different processes occur - graft union formation and adventitious rooting. The aim of this study was to find out, what changes in starch content and chlorophyll fluorescence occur in rootstock with or without apical bud during propagation of elepidote rhododendron cultivar 'Cunningham's White' by cutting grafts.Due to wounding stress a decrease with small fluctuations in Fv/Fm parameter in rootstock [with and without apical bud] leaves was observed for first 18 days of experiment. Afterwards the callus bridge formation was observed and Fv/Fm started to increase. In first days of experiment increase in starch content was observed, followed by decrease until Day 21 (with apical bud) or Day 18 (without apical bud). These changes can be related to graft union formation. For last 9 or 12 days of experiment starch accumulated to be used in rooting process. In conclusion, the presence of apical bud probably can delay graft union formation, but further studies are necessary to prove this hypothesis. LIGHT QUALITY AFFECTS FLOWERING INDUCTION IN FRAGARIA VESCA 'HAWAII-4' Marja Rantanen, K. Mouhu, P. Elomaa, P. Palonen, T. Hytönen Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland E-mail: marja.rantanen@helsinki.fi 58 X X I V S P P S C O N G R E S S 2 0 1 1
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Foreword eword ord A B S T R A C T
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Program of the Congress gram of the
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Page 11 and 12: Plenary session 1 A B S T R A C T B
Page 13 and 14: Plenary session 2 THE BIOLOGY OF AL
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Page 27 and 28: Pleanary session 4 A B S T R A C T
Page 29 and 30: Invited talk 4 A B S T R A C T B O
Page 31 and 32: Session 5.Cell biology A B S T R A
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Page 37 and 38: Session 7.Development A B S T R A C
Page 39 and 40: Session 8.Signalling A B S T R A C
Page 41 and 42: Pleanary session 6 A B S T R A C T
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Page 67 and 68: Sapporo Medical University, Sapporo
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