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A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S We studied the effects of light quality on long day flowering Fragaria vesca 'Hawaii-4'. Seedlings were subjected to short day (SD) plus low intensity daylength extension by blue (B), far-red (FR), red (R), or incandescent light (INC). Flowering integrator gene FLOWERING LOCUS-T (FT) and floral identity gene FRUITFULL (FUL) were up-regulated after one week in INC and FR treatments. In contrast, we could not detect expression of FT and FUL in R and SD treatments during the treatment period of five weeks. In a further study at higher temperature, both genes were expressed similarly to previous results in R, SD and INC treatments while in B light both genes were expressed at low level. Furthermore, INC and FR treated plants flowered early and flowering response in B light was intermediate, whereas both R and SD treated plants flowered significantly later. The results show that the expression of FT and FUL correlates with flowering initiation in F.vesca 'Hawaii-4'. Strong responses to FR and R light and milder response to B light indicate that phytochromes are the major photoreceptors controlling flowering. Ongoing functional analysis of FT will confirm its role in the light regulated flowering induction. THYLAKOID CALCIUM SENSING RECEPTOR AS A REGULATOR OF PHOTOSYNTHETIC LIGHT REACTIONS Markus Nurmi, Saijaliisa Kangasjärvi, Eva-Mari Aro University of Turku, Turku, Finland E-mail: mjnurm@utu.fi Calcium sensing receptor (CaS) is a 40 kDa phosphoprotein on the photosynthetic thylakoid membrane. It is phosphorylated by STN8 kinase in a C-terminal stroma-exposed motif, which predicts interactions with 14-3- 3-proteins and with proteins containing fork-head associated domains. Analysis of mechanically fractionated thylakoid membranes by immunoblotting revealed differential light-intensity-dependent localization for CaS. In darkness and under low light conditions, CaS resides in granal membranes, whereas under bright light, high amounts of CaS is detected in stromal regions of the thylakoid membrane. Results obtained by immunoblotting of 2D Blue Native gels suggest that CaS interacts with Photosystem II (PSII). Comparative transcript profiling shows increased transcript levels for the PSII oxygen evolving complex protein PsbO2 and for two Photosystem I core proteins in knock-out cas plants. Intriguingly, publicly available co-expression data also points to a connection between CaS and these components. The cas mutant also shows a distinct up-regulation of PGRL1 encoding a key component of cyclic electron transfer. Furthermore, the steady-state protein level of PGRL1 follows the phosphorylation status of CaS in differentially light-treated leaves, suggests that these two proteins are functionally connected. Altogether, CaS seems to be involved in the regulation of photosynthetic light reactions in the changing environmental cues. ACCLIMATION RESPONSE OF BARLEY PLANTS GROWN UNDER LOW AND HIGH PAR TO SUPPLEMENTAL UV-A EXPOSURE Michal Štroch, Alena Kotyzová, Jakub Nezval, Zdeněk Nosek, Václav Karlický, Rostislav Páník, Vladimír Špunda Department of Physics, Faculty of Science, Ostrava University, Ostrava, Czech Republic E-mail: michal.stroch@osu.cz The dynamics of the acclimation response of barley assimilation apparatus was examined after transfer of plants grown under low and high PAR to conditions with the same level of PAR and supplemental UV-A radiation. The aim of experiment was to evaluate, how the level of PAR during growth of barley plants will affect the induction of regulatory and protective mechanisms of the assimilation apparatus (e.g. epidermal UV-shielding, utilization of absorbed light energy within photosystem II, xanthophyll cycle activity) in the course of UV-A exposure. Spring barley (Hordeum vulgare L. cv. Bonus) was grown under low and high PAR (50 and 1000 µmol m -2 s -1 ) in the absence of UV radiation for 8 days. Then the plants were exposed to UV-A radiation (8 W m -2 for 16 h per day) for the next 6 days. During the period of UV-A exposure we monitored UV-shielding efficiency, functional state of photosystem II and composition of photosynthetic pigments and UV-absorbing compounds. The results are discussed with regard to the effect of supplemental UV-B exposure of barley plants acclimated to low and high PAR. 59 X X I V S P P S C O N G R E S S 2 0 1 1
A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S DYNAMIC REGULATION OF LIGHT HARVESTING AND ENERGY DISTRIBUTION BY THESTN7 KINASE AND NPQ PROMOTES ACCLIMATION TO FLUCTUATING LIGHT CONDITION Michele Grieco, Mikko Tikkanen, Eva-Mari Aro University of Turku, Turku, Finland E-mail: micrie@utu.fi Studies on the function of light-harvesting complex II (LCHII) phosphorylation for the acclimation to changing light conditions in plants have been focused mainly on different quality of light, in relation to state transitions. However, natural light conditions mainly face changes in the quantity of white light requesting acclimation strategies for both high and low light in short and long term periods. Here we have investigated the role of the thylakoid kinases STN7 and STN8 as well as the regulatory protein PsbS in dynamic acclimation of Arabidopsis thaliana plants when grown under short-term fluctuations in the intensity of white light. It is concluded that the lack of the dynamic control of excitation energy distribution to PSII and PSI in the absence of the STN7 kinase induces controversial commands within short time periods to initiate signalling cascades thus interfering a proper adjustment of photosystems stoichiometry. Indeed, the STN7 mutants when grown under constant light intensity can compromise the lack of the STN7 kinase by drastically decreasing the PSII/PSI ratio. On the contrary, under fluctuating white light such acclimation mechanism is prohibited and the STN7 mutants show stunted growth phenotype. EFFECT OF INTEGRATION OF CYANOBACTERIAL PCS GENE ON THE PROTEOME OF CISGENICALLY TRANSFORMED ANABAENA SP. PCC 7120 Neha Chaurasia 1,2 , Yogesh Mishra 1 , L.C.Rai 1 1 Molecular Biology Section, Laboratory of Algal Biology, Center of Advanced Study in Botany, Banaras Hindu University, Varanasi, India 2 Department of Biotechnology and Bioinformatics, North Eastern Hill University, Shillong, India E-mail: cyanoneha@gmail.com Cyanobacteria, the photosynthetic prokaryotes present in varied habitats are bestowed with unique ability to fix nitrogen and carbon dioxide simultaneously. To proliferate in nature, cyanobacteria develop a multitude of stress tolerant strategies. The present study provides first hand information of the impact of cisgenic transformation with a cyanobacterial gene phytochelatin synthase (pcs) over the proteome of the transformed cyanobacterium Anabaena sp. PCC 7120. This gene was first cloned in E.coli and its tolerance against multiple abiotic stresses was examined. Following which Anabaena sp. PCC 7120 was transformed using an integrative expression vector, pFPN containing the pcs gene expressed from astrong cyanobacterial psbA1 promoter. The transformed strain AnFPN-pcs demonstrated a better growth and nitrogen fixing ability over wild type when challenged with UV-B, salt, heat, copper, carbofuran and cadmium. The proteome of transformed strain AnFPN-pcs was compared with wild type Anabaena sp PCC 7120 using 2DE and MALDI-TOF/ MS. Most of the proteins related to cell metabolism were upregulated in transformed strain as compared to the control cells expect the downregulation of transketolase and ketolacid reductoisomerase. Thus the stress induced protein genes hold potential for the development of multiple stress tolerant organisms. FUNCTION OF FERREDOXIN-NADP + -OXIDOREDUCTASE (FNR) LEAF ISOFORMS IN ARABIDOPSIS THALIANA Nina Lehtimäki, Minna Lintala, Yagut Allahverdiyeva-Rinne, Eva-Mari Aro, Paula Mulo Molecular Plant Biology, Department of Biochemistry and Food Chemistry, University of Turku, Turku, Finland E-mail: nkleht@utu.fi 60 X X I V S P P S C O N G R E S S 2 0 1 1
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Foreword eword ord A B S T R A C T
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Program of the Congress gram of the
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A B S T R A C T B O O K - P R O G R
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Page 11 and 12: Plenary session 1 A B S T R A C T B
Page 13 and 14: Plenary session 2 THE BIOLOGY OF AL
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Page 19 and 20: Session 2. Systems biology and -omi
Page 21 and 22: Session 2. Systems biology and -omi
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Page 27 and 28: Pleanary session 4 A B S T R A C T
Page 29 and 30: Invited talk 4 A B S T R A C T B O
Page 31 and 32: Session 5.Cell biology A B S T R A
Page 33 and 34: Session 6.Organelle biology A B S T
Page 35 and 36: Session 6.Organelle biology A B S T
Page 37 and 38: Session 7.Development A B S T R A C
Page 39 and 40: Session 8.Signalling A B S T R A C
Page 41 and 42: Pleanary session 6 A B S T R A C T
Page 51 and 52: Session 13.Pathogen defense and pla
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Page 67 and 68: Sapporo Medical University, Sapporo
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Page 97 and 98: Sponsors onsors ors A B S T R A C T
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