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A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S by using the yeast one-hybrid screening. We showed that PIF7 functions as a transcriptional repressor for DREB1 expression under circadian control. In contrast, CAMTA proteins activate expression of the GUS reporter gene driven by the DREB1 promoters in transient experiments using Arabidopsis protoplasts. We are analyzing interaction between PIF7 and CAMTA proteins by using the BiFC assay. THE TISSUE-SPECIFIC GLYCINE BETAINE ACCUMULATION IN BARLEY PLANTS - HOW DO BARLEY PLANTS UTILIZE THE GLYCINE BETAINE? Takashi Fujiwara, Shiro Mitsuya, Tasuku Hattori, Tetsuko Takabe Nagoya University, Chikusa-ku, Nagoya, Japan E-mail: takafuji@nuagr<strong>1.</strong>agr.nagoya-u.ac.jp The accumulation of glycine betaine (GB) is one of the adaptive strategies to adverse salt stress conditions. It has been demonstrated that some plants accumulate GB in response to salt stress and various analyses of enzymes that catalyze the GB synthesis were performed. However, it remains unknown where the plants accumulate GB at plant level. Thus, it would be difficult to clarify the role of GB in plants. Since GB is not degraded in plants, accumulation patterns of GB at plant levels are regulated by the synthesis and transport. In this report, we have investigated the distribution of GB at organ level, tissue localization of BADH protein that catalyzes the last step in the GB synthesis and that of HvProT2 mRNA, betaine transporter in barley plants. GB was increased by salt treatment and accumulated more in young leaves than in older leaves. BADH proteins were expressed around the xylem vessels of leaves, and in the pericycle cells of roots. Moreover, HvProT2 mRNA was expressed in the mestome sheath and lateral root cap cells. These results indicate that barley plants accumulate GB at vascular tissues under salt stress conditions. We discuss the possible function of GB in barley plants. EVALUATION OF SUGARBEET MONOGERM O-TYPE LINES FOR SALINITY TOLERANCE AT VEGETATIVE STAGE Zahra Abbasi, Ahmad Arzani and Mohammad Mehdi Majidi Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan, Iran E-mail: z.abbasi@ag.iut.ac.ir Sugar beet is a salt tolerant crop species that is cultivated in the Iranian areas where salinity can be a serious threat. Screening of genotypes for salinity tolearance is difficult in field due to heterogeneity of physical and chemical properties of soil. To evaluate the salinity tolerance of 21 sugarbeet monogerm Otypes lines a greenhouse experiment was conducted using a split plot design with 3 replications. Two levels of control (2 dS m-1) and salinity (16 dS m-1) were used as main plots and 21 lines were allocated to subplots. In each plastic pot (18cm diameter) 24 monogerm seeds were sown in perlit bed and a drop irrigation system was implemented. Germination percentage, germination rate, seedling survival percentage, chlorophyl content, dry weight of shoots and roots after 8 weeks of the treatments were evaluated.The results of analysis of variance showed the significant effects of salinity on the tested traits. Sugar beet genotypes differed significantly (P
A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S Arabidopsis RNA polymerase II (RNAPII) C-terminal domain (CTD) phosphatases regulate stress-responsive gene expression and plant development via the dephosphorylation of serine (Ser) residues of the CTD. Some of these phosphatases (CTD phosphatase-like 1 (CPL1) to CPL3) negatively regulate ABA and stress responses. We isolated AtCPL5, a cDNA encoding a protein containing two CTD phosphatase domains (CPDs). To characterize AtCPL5, we analyzed the gene expression patterns and subcellular protein localization, investigated various phenotypes of AtCPL5-overexpressors and knockout mutants involved in ABA and drought responses, performed microarray and RNA hybridization analyses using AtCPL5-overexpressors, and assessed the CTD phosphatase activities of the purified AtCPL5 and each CPD of the protein. Transcripts of the nucleus-localized AtCPL5 were induced by ABA and drought. AtCPL5-overexpressors exhibited ABA-hypersensitive phenotypes (increased inhibition of seed germination, seedling growth, and stomatal aperture), lower transpiration rates upon dehydration, and enhanced drought tolerance, while the knockout mutants showed weak ABA hyposensitivity. AtCPL5 overexpression changed the expression of numerous genes, including those involved in ABA-mediated responses. In contrast to Ser-5-specific phosphatase activity of the negative stress response regulators, purified AtCPL5 and each CPD of the protein specifically dephosphorylated Ser-2 in RNAPII CTD. We conclude that AtCPL5 is a unique CPL family protein that positively regulates ABA-mediated development and drought responses in Arabidopsis. CALLUS INDUCTION AND FATTY ACID PROFILES OF JATROPHA CURCAS INDUCED CALLI ChongSiang Tee 1 , ThenSoong Siow 1 , Adelin Ting Su Yien 2 1 Department of Biological Science, Faculty of Science, Universiti Tunku Abdul Rahman, Jalan Universiti, Bandar Barat, Kampar, Perak, Malaysia 2 School of Science, Monash University, Jalan Lagoon Selatan, Bandar Sunway, Selangor Darul Ehsan, Malaysia. E-mail: teecs@utar.edu.my Jatropha curcas, a biodiesel production plant, is intensively cultivated in many countries. The main objectives of this study were to induce callus from various types of J. curcas explants and study the fatty acid content of the induced calli. In this study, different types of auxins at various concentrations were used to induce callus. In addition, fatty acid compositions and total oil content in the induced calli were also investigated. It was observed that 4 µM of picloram, 4 µM of dicamba and 2 µM of 2,4-D, cotyledon and petioles explants, respectively. For leaf explants, 2,4-D was used in combination with cytokinin to induce callus. Besides, embryogenic calli induced from cotyledon explants using MS medium containing 4 µM dicamba. The fatty acid analysis revealed that fatty acid compositions in the induced calli were similar to that of seed kernels particularly the fatty acid profile of embryogenic calli. Generally, induced calli had lower oil content than seed kernels. Among all types of induced calli studied, embryogenic calli had higher total oil content than non- embryogenic calli but significantly lower than the seed kernels. NEW PERSPECTIVE IN PLANT VITAMIN D PRODUCTION Daniele Silvestro 1 , Christina Fredslund 1 , Rie Bak Jäpelt 2 , Poul Erik Jensen 1 1 Department of Plant Biology and Biotechnology, Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark 2 Division of Food Chemistry, National Food Institute, Technical University of Denmark, Kongens Lyngby, Denmark E-mail: dasi@life.ku.dk The presence of vitamin D compounds has been established in a few plant species and could represent an alternative source of vitamin D. Interestingly, the metabolic pathway leading to the formation of vitamin D in plants is basically unknown. The enzyme Δ5,7-sterol-Δ7-reductase (7DHCR) is known to catalyze the conversion of 7DHC into cholesterol in vertebrates while in plants a similar enzyme (DWARF5) converts the Δ5,7-sitosterol (7DHS) into sitosterol. The structure similarity should make possible the conversion of 7DHS into vitamin D (vitamin D5) by exposure to UV B light. To verify if this conversion is occurring in plants A. thaliana dwarf5 mutants have been exposed to UV B light. This mutant is accumulating Δ5,7-sterols (mainly 7DHS) due to a mutation in the Δ7-reductase and it displays a dwarf phenotype due to the down regulation of the brassinosteroid biosynthesis. Moreover two cDNA sequences highly homologous to A. thaliana DWARF5 were isolated from S. lycopersicum. The activity of the codified enzymes was assessed by enzymatic assay via expression studies in yeast and plant. The results on yeast made by GC-MS sterol characterization of the transformants 82 X X I V S P P S C O N G R E S S 2 0 1 1
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Foreword eword ord A B S T R A C T
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Program of the Congress gram of the
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A B S T R A C T B O O K - P R O G R
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A B S T R A C T B O O K - P R O G R
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Plenary session 1 A B S T R A C T B
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Plenary session 2 THE BIOLOGY OF AL
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Session 1. Biotechnology and synthe
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Session 1. Biotechnology and synthe
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Session 2. Systems biology and -omi
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Session 2. Systems biology and -omi
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A B S T R A C T B O O K - A B S T R
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A B S T R A C T B O O K - A B S T R
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Pleanary session 4 A B S T R A C T
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Invited talk 4 A B S T R A C T B O
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Page 37 and 38: Session 7.Development A B S T R A C
Page 39 and 40: Session 8.Signalling A B S T R A C
Page 41 and 42: Pleanary session 6 A B S T R A C T
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Page 67 and 68: Sapporo Medical University, Sapporo
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Page 93 and 94: A B S T R A C T B O O K - L I S T O
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Page 97 and 98: Sponsors onsors ors A B S T R A C T
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