1. Front Cover.cdr - CORE

A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S
DYNAMIC REGULATION OF LIGHT HARVESTING AND ENERGY DISTRIBUTION BY
THESTN7 KINASE AND NPQ PROMOTES ACCLIMATION TO FLUCTUATING LIGHT
CONDITION
Michele Grieco, Mikko Tikkanen, Eva-Mari Aro
University of Turku, Turku, Finland
E-mail: micrie@utu.fi
Studies on the function of light-harvesting complex II (LCHII) phosphorylation for the acclimation to
changing light conditions in plants have been focused mainly on different quality of light, in relation to state
transitions. However, natural light conditions mainly face changes in the quantity of white light requesting
acclimation strategies for both high and low light in short and long term periods. Here we have investigated
the role of the thylakoid kinases STN7 and STN8 as well as the regulatory protein PsbS in dynamic
acclimation of Arabidopsis thaliana plants when grown under short-term fluctuations in the intensity of
white light. It is concluded that the lack of the dynamic control of excitation energy distribution to PSII and
PSI in the absence of the STN7 kinase induces controversial commands within short time periods to initiate
signalling cascades thus interfering a proper adjustment of photosystems stoichiometry. Indeed, the STN7
mutants when grown under constant light intensity can compromise the lack of the STN7 kinase by
drastically decreasing the PSII/PSI ratio. On the contrary, under fluctuating white light such acclimation
mechanism is prohibited and the STN7 mutants show stunted growth phenotype.
EFFECT OF INTEGRATION OF CYANOBACTERIAL PCS GENE ON THE PROTEOME OF
CISGENICALLY TRANSFORMED ANABAENA SP. PCC 7120
Neha Chaurasia 1,2 , Yogesh Mishra 1 , L.C.Rai 1
1 Molecular Biology Section, Laboratory of Algal Biology, Center of Advanced Study in Botany, Banaras Hindu University, Varanasi, India
2 Department of Biotechnology and Bioinformatics, North Eastern Hill University, Shillong, India
E-mail: cyanoneha@gmail.com
Cyanobacteria, the photosynthetic prokaryotes present in varied habitats are bestowed with unique ability
to fix nitrogen and carbon dioxide simultaneously. To proliferate in nature, cyanobacteria develop a
multitude of stress tolerant strategies. The present study provides first hand information of the impact of
cisgenic transformation with a cyanobacterial gene phytochelatin synthase (pcs) over the proteome of the
transformed cyanobacterium Anabaena sp. PCC 7120. This gene was first cloned in E.coli and its tolerance
against multiple abiotic stresses was examined. Following which Anabaena sp. PCC 7120 was transformed
using an integrative expression vector, pFPN containing the pcs gene expressed from astrong
cyanobacterial psbA1 promoter. The transformed strain AnFPN-pcs demonstrated a better growth and
nitrogen fixing ability over wild type when challenged with UV-B, salt, heat, copper, carbofuran and
cadmium. The proteome of transformed strain AnFPN-pcs was compared with wild type Anabaena sp PCC
7120 using 2DE and MALDI-TOF/ MS. Most of the proteins related to cell metabolism were upregulated in
transformed strain as compared to the control cells expect the downregulation of transketolase and ketolacid
reductoisomerase. Thus the stress induced protein genes hold potential for the development of
multiple stress tolerant organisms.
FUNCTION OF FERREDOXIN-NADP + -OXIDOREDUCTASE (FNR) LEAF ISOFORMS IN
ARABIDOPSIS THALIANA
Nina Lehtimäki, Minna Lintala, Yagut Allahverdiyeva-Rinne, Eva-Mari Aro, Paula Mulo
Molecular Plant Biology, Department of Biochemistry and Food Chemistry, University of Turku, Turku, Finland
E-mail: nkleht@utu.fi
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