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A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S 2 Biological Function Division, National Food Research Insitute, Tsukuba, Ibaraki, Japan 3 Joint BioEnergy Institute, Feedstocks Division, Lawrence Berkeley National Laboratory, Emeryville, California, USA E-mail: evaknoch@life.ku.dk CAZy glycoside hydrolase family 17 is one of the plant dominant GH families, and includes glucan 1,3-βglucosidase, glucan endo-1,3-β-glucanase and lichenase. In Arabidopsis there are 51 genes in this family and in silico analysis suggests that several members are highly coexpressed with a glycosyltransferase from CAZy family 31 involved in arabinogalactan protein (AGP) biosynthesis. We are interested in the relationship between these enzymes with respect to AGP biosynthesis. We chose the glycosyl hydrolase (GH17) which shows the highest coexpression profile for further investigation. This GH17 is expressed in the embryo and shoot apex in Arabidopsis. The protein sequence consists of an Nterminal signal sequence, a GH17 catalytic domain, CBM43 domain, and a site for GPI-anchoring on the Cterminus. The protein sequence between the catalytic domain and the CBM domain also contains SPSPSSSP sequence which is a potential motif for attachment of arabinogalactan side chains. We are interested to see whether this GH17 is an acceptor-peptide for type II arabinogalactan attachment and/or it is involved in the modification of AGP on the plasma membrane. We are investigating these possible functions by analysis of Arabidopsis knockout mutants, and hydrolase assays using recombinant protein expressed in N.benthamiana and E.coli. DUAL-TARGETING OF A EUKARYOTIC TRANSCRIPTION FACTOR, AT2G44940 Lan Yin, Kirsten Krause Department of Arctic and Marine Biology,University of Tromsø, Tromsø, Norway E-mail: lyi000@uit.no In recent years, a growing number of proteins have been shown to be localized in both nuclear and plastid compartments [1]. Since such proteins might be involved in chloroplast-nuclear crosstalk,an in silico-based screening of transcription factors from Arabidopsis and rice was carried out to find the putative proteins that are both targeted to plastids and nuclear [2] (Schwacke R. et al., 2007). Transcription factor At2g44940 (TF1) is one of the putative proteins that are dual-targeted to both chloroplast and nucleus. Transient transformation of tobacco protoplasts showed indeed that a GFP fusion protein is targeted to the nucleus and the chloroplasts in the same cell. Higher resolution analysis revealed, moreover, that the TF1 protein was co-localized with a plastid DNA marker. SDS-PAGE and Western Blot results indicated that precursor TF1 overexpressed in E. coli was a protein around 40kDa, its mature protein was around 35kDa, both being bigger than the predicted MW 32kDa and 27kDa. A protein of 35kD was detected in the chloroplast stroma as well as in isolated nuclei, indicating that a processed form might prevail in both cell compartments. In addition, the semi-quantitative RT-PCR of the TF1 transcript indicated that the expression of TF1 was only moderately expressed in darkness but increased significantly after transfer to light. The significance of this will be further investigated. Reference [1] Krause and Krupinska, Trends Plant Sci. 2009 [2] Schwacke et al., Mol. Genet. Genomics2007 INFLUENCE OF SALINITY ON ANTIOXIDANT ACTIVITY IN CANOLA (BRASSICA NAPUS L.) CULTIVARS E. Shahbazi, A. Arzani and G. Saeidi Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan, Iran E-mail: es_shahbazi@yahoo.com The effects of salt stress on germination, seedling growth parameters (root and shoot lengths, root and shoot fresh and dry weights) and activity of antioxidant enzymes in leaves of six cultivars of canola (Brassica napus L.) were investigated. Two F1 hybrids (Hyola401, Hyola330) and four open pollinated cultivars (Zarfam, Okapi, RGs003 and Sarigol) were used in this study. Seeds were germinated under various level of salinity 0, 50, 100, 150 and 200 mM NaCl concentrations. Results showed that an increase in NaCl 63 X X I V S P P S C O N G R E S S 2 0 1 1
A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S concentrations progressively inhibited seed germination. Hyola401 showed the greatest germination percentage at all salinity levels. Seedling growth parameters were affected by salt stress particularly at 150 mM and 200mM. Leaf antioxidant activities of SOD, APX and GR were increased by salinity increment up to 150mM while they were decreased in 200 mM NaCl concentration. Although constitutive levels of activity of antioxidative enzymes were almost the same among canola cultivars, Hyola401 induced antioxidant enzyme activities more efficiently when subjected to NaCl treatment. Among the tested cultivars, F1 hybrid 'Hyola401' could be considered as salt tolerance as possessing superior germination percentage, seedling growth parameters, antioxidant activities under salinity stress. On the other hand, F1 hybrid 'Hyola330' performed inferior in those aspects and was the most susceptible cultivars to salinity stress. THE ROLE OF RHOMBOID PROTEASES IN ARABIDOPSIS Elinor P. Thompson 1 , Beverley J. Glover 2 1 University of Greenwich, London, United Kingdom 2 University of Cambridge, Cambridge, United Kingdom E-mail: te30@gre.ac.uk Rhomboid proteins constitute a family of membrane-bound serine proteases, which are found in nearly every genome so far sequenced. Both eukaryotic and prokaroytic rhomboids mediate communication or interactions between cells (eg, during infection) by releasing or activating membrane-anchored substrate proteins. Plant rhomboids are not yet well characterised. In Arabidopsis, mutation of the rhomboid 'KOM' results in malformed pollen; enzymatic activity of another has been shown in vitro. Investigating several members of the multigene family in Arabidopsis, we found that other rhomboids besides KOM affected development of floral organs. Indeed, several rhomboids' promoter-GUS constructs yield pollen- and seedlocalised staining. Other family members are expressed more widely, their transcripts being found in many tissues, and a few Arabidopsis rhomboid genes contain predicted organelle-targeting presequences. GFPtagging of a chloroplast rhomboid suggests it is situated in the organelle's outer membrane; the null mutant of this protein shows both fertility and photosynthetic defects. We discuss our findings in relation to the different subtypes of rhomboids, presence of transcript splice-variants, and evolution of rhomboids' roles in plants and other organisms. CYTOSOLIC NADP-ISOCITRATE DEHYDROGENASE (ICDH) IS DIFFERENTIALLY REGULATED BY REACTIVE NITROGEN SPECIES (RNS) IN PHOTOSYNTHETIC AND NON- PHOTOSYNTHETIC ARABIDOPSIS ORGANS M. Leterrier 1 , J.B. Barroso 2 , R. Valderrama 2 , J.C. Begara-Morales 2 , B. Sanchez-Calvo 2 , M. Chaki 1 , J.M. Palma 1 , F.J. Corpas 1 1 Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín, CSIC, Granada, Spain 2 Grupo de Señalización Molecular y Sistemas Antioxidantes en Plantas, Unidad Asociada al CSIC (EEZ), Departamento de Bioquímica y Biología Molecular, Universidad de Jaén, Jaén, Spain E-mail: josemanuel.palma@eez.csic.es NADPH is an essential component in the cellular homeostasis and its regeneration is critical for reductive biosynthesis and detoxification processes. One of the most important NADPH cell sources is the enzyme NADP-isocitrate dehydrogenase (ICDH) which concomitantly converts isocitrate into alpha-ketoglutarate. Arabidopsis contains three genes coding for cytosolic, mitochondrial/chloroplastic and peroxisomal NADP- ICDH isoenzymes. The cytosolic isoenzyme is the most abundant representing more that 90% of the total activity. Biochemical comparative analyses revealed that the kinetic parameters (Km and Vmax) of cytosolic NADP- ICDH were different in photosynthetic and non-photosynthetic organs of Arabidopsis plants. Moreover, the activity showed a differential regulation in response to the treatment with reducing agents, nitric oxide (NO), and oxidants such as peroxynitrite and hydrogen peroxide (H2O2). Thus, H2O2 did not affect the NADP- ICDH activity in any organ; however, reduced glutathione inhibited the activity in leaves but not in roots. On the other hand, S-nitrosoglutathione (an NO donor) provoked inhibition of NADP-ICDH in both organs and similar behaviour was found with peroxynitrite. Taken together, these data indicate that cytosolic NADP- 64 X X I V S P P S C O N G R E S S 2 0 1 1
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Foreword eword ord A B S T R A C T
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Program of the Congress gram of the
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A B S T R A C T B O O K - P R O G R
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A B S T R A C T B O O K - P R O G R
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Plenary session 1 A B S T R A C T B
Page 13 and 14: Plenary session 2 THE BIOLOGY OF AL
Page 15 and 16: Session 1. Biotechnology and synthe
Page 17 and 18: Session 1. Biotechnology and synthe
Page 19 and 20: Session 2. Systems biology and -omi
Page 21 and 22: Session 2. Systems biology and -omi
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Page 27 and 28: Pleanary session 4 A B S T R A C T
Page 29 and 30: Invited talk 4 A B S T R A C T B O
Page 31 and 32: Session 5.Cell biology A B S T R A
Page 33 and 34: Session 6.Organelle biology A B S T
Page 35 and 36: Session 6.Organelle biology A B S T
Page 37 and 38: Session 7.Development A B S T R A C
Page 39 and 40: Session 8.Signalling A B S T R A C
Page 41 and 42: Pleanary session 6 A B S T R A C T
Page 51 and 52: Session 13.Pathogen defense and pla
Page 53 and 54: Session 13.Pathogen defense and pla
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Page 67 and 68: Sapporo Medical University, Sapporo
Page 91 and 92: SCF = A B S T R A C T B O O K - A B
Page 93 and 94: A B S T R A C T B O O K - L I S T O
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Page 97 and 98: Sponsors onsors ors A B S T R A C T
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