A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S 53 X X I V S P P S C O N G R E S S 2 0 1 1 Abstracts of Posters
A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S THE INDUCTION AND GROWTH OF POTATO (SOLANUM TUBEROSUM. L) MICROTUBERS, SANTE CULTIVAR, RESPONSE TO THE DIFFERENT CONCENTRATIONS OFBAP AND SUCROSE Alireza Iranbakhsh 1 , Mostafa Ebadi 2 1 Islamic Azad University, Aliabad Katool, Golestan Province, Iran 2 Islamic Azad University, Damghan Branch, Semnan Province, Iran E-mail:iranbakhshar@yahoo.com The effects of different concentrations of Banzil Amino Purin (BAP) and sucrose as induction combinations on microtuber formation and the time of this process, numbers and fresh and dry weight of microtubers were investigated. A two-stage culture was used to study the effect of hormonal and sucrose treatments. In the first stage, a liquid MS medium containing 0.5 mgl -1 BAP + 0.4 mgl -1 GA3 + 20 gl -1 sucrose was used for the increase of branches. The cultures of single node were grown against white light (4000 - 5000 LUX) and on sucrose for one month. In the second stage, microtuber formation induced on fluid MS medium containing different concentrations of sucrose (30, 40, 60, 80 mgl -1 ) and BAP (1, 2, 5, 10 mgl -1 ) was used in continuous darkness. Microtuber formation was investigated within 10 weeks after induction. CHARACTERISATION OF SECRETED COMPOUNDS IN NORWAY SPRUCE TISSUE CULTURE MEDIUM BY CHROMATOGRAPHY AND MASS SPECTROMETRY Gwe G.Chenyi 1 , S.Chong 2 , S.Koutaniemi 2 , M.Tenkanen 2 , S.C.Fry 3 , A.Kärkönen 1,4 1 Department of Agricultural Sciences, University of Helsinki, Finland 2 Department of Food and Environmental Sciences, University of Helsinki, Finland 3 Institute of Molecular Plant Sciences, University of Edinburgh, United Kingdom 4 MTT Agrifood Research Finland, Jokioinen, Finland E-mail: gilbert.gwe@helsinki.fi Oligosaccharides secreted into the culture medium of suspension cultures have been shown to be similar to those in cell walls [1, 2]. Some secreted oligosaccharides may play a crucial role in plant growth and development. For example, fungal cell wall-derived oligosaccharides elicit the generation of a defenceassociated H2O2 burst in extracellular lignin-producing Norway spruce cell cultures [3, 4]. We hypothesise that soluble oligosaccharides present in the culture medium of spruce cells regulate the constant low levels of H2O2 present during extracellular lignin formation. Cultured spruce cells were fed with [U-14C]glucose and treated subsequently with H2O, elicitor, KI or KCl. The culture medium compounds were separated by paper chromatography and high voltage paper electrophoresis. The results show varying amounts of certain compounds in different treatments. The positively charged compounds have been further purified by cation exchange chromatography. The identification (mass spectrometry) of compounds is underway and their involvement in plant growth and development will be investigated. References [1] McNeil et al., Annu. Rev. Biochem. 1984 [2] York et al., Methods Enzymol. 1985 [3] Kärkönen and Fry, J. Exp. Bot. 2006 [4] Kärkönen et al., Planta 2009 POLYPHOSPHATES IN PLANTS Hélène Pélissier Combescure, Elizabeth Nees Ahm, Tom Hamborg Nielsen Department of Plant Biology and Biotechnology, Faculty of Life Sciences, Copenhagen University, Copenhagen, Denmark E-mail: hepel@life.ku.dk Organisms can store phosphate as polyphosphate, a linear polymer of phosphate residues linked by highenergy bonds. Polyphosphate serves as an energy reservoir, and chelates heavy metal ions. In bacteria, it plays a role in stress responses, gene regulation, motility and virulence. The polyphosphate kinase (PPK1), 54 X X I V S P P S C O N G R E S S 2 0 1 1
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