1. Front Cover.cdr - CORE
1. Front Cover.cdr - CORE
1. Front Cover.cdr - CORE
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A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S<br />
anion channels. The ATP for proton pumping is supplied mostly from mitochondrial respiration; however, a<br />
partial inhibition with DCMU implies a role of GC photosynthetic electron transport in the ATP supply.<br />
In order to investigate whether CHT affects the photosynthetic ATP production of GCs, the lightdependence<br />
of the photosynthetic electron transport rate of individual GCs was assayed. Vicia faba leaves<br />
were treated with CHT and chlorophyll fluorescence parameters of GCs were measured by Microscopy-PAM<br />
chlorophyll fluorometer.In addition, to test the possible effect of CHT on the activity of ion channels, Vicia<br />
GC protoplasts were investigated by patch clamp technique.<br />
Acknowledgements<br />
This work was supported by the Hungarian Scientific Research Fund (Grant. no. OTKA K 81471).<br />
DEMONSTRATION OF DIFFERENTIALLY REGULATED PROTEINS DURING THE<br />
INTERACTION BETWEEN PHYTOPHTHORA INFESTANS AND A TRANSGENIC POTATO<br />
EXPRESSING RPI-BLB1 FROM SOLANUM BULBOCASTANUM<br />
B. Colignon 1,2 , M. Raes 2 , E. Delaive 2 , M Dieu 2 , B .Watillon 1 , S. Mauro 1<br />
1 Département Sciences du Vivant, Centre wallon de Recherches agronomiques, Gembloux, Belgium<br />
2 Unité de Recherche en Biologie Cellulaire, Facultés Universitaires Notre-Dame de la Paix, Namur, Belgium<br />
E-mail: b.colignon@cra.wallonie.be<br />
Solanum tuberosum (cv Desirée) is susceptible to the attack of Phythophthora infestans Mont de bary. The<br />
Rpi-blb1 gene from Solanum Bulbocastanum, encoding a cytoplasmic NBS-LRR protein which recognizes<br />
Phytophthora effectors in the “gene for gene” model, confers resistance to the pathogen. We made a<br />
dynamic total leaf proteome comparison of the wild type and transgenic potato cultivars using a 2D DIGE<br />
strategy coupled to MS identification. Proteomic data revealed 33 and 21 proteins to be differentially<br />
expressed in transgenic and wild type challenged leaves respectively.<br />
Using principal component analysis we could clearly separate challenged and healthy leaf tissues. By using<br />
a systems biology approach, we could map some of the identified proteins into pathways involved in the<br />
interaction with Phytophthora infestans.<br />
SIGNIFICANCE OF FIRST BURST OF ROS AND ETHYLENE AS SIGNALING MOLECULES IN<br />
PATHOGEN-INDUCED THEIR BIPHASIC GENERATION<br />
Soo Jin Wi, Ky Young Park<br />
Department of Biology, Sunchon National University, Jeollanam-do, South Korea<br />
E-mail: plpm@sunchon.ac.kr<br />
We investigated the relationship between ROS and ethylene in response to biotic stress with the fungal<br />
pathogen using transgenic tobacco plants, in which ethylene biosynthesis or signaling or ROS production<br />
was impaired. It was observed that wild-type plants exhibited a gene-specific expression of NtACS<br />
members of ACC synthase, which were regulated in a time-dependent manner. Pathogen-induced<br />
expression of NADPH oxidase, RbohD and RbohF, and ROS accumulation at phase I were significantly<br />
suppressed in transgenic plants, in which ethylene biosynthesis and signaling were impaired. Biphasic<br />
ethylene production was also inhibited, especially at 1h, in stress-tolerant transgenic plants with impairment<br />
of RbohD and RbohF expression. The growth of Phytophthora, determined by expression of its 18s rRNA,<br />
was significantly reduced in transgenic plants with the impairment of ROS generation and ethylene<br />
biosynthesis and signaling pathway. To determine the physiological function of first burst of ethylene and<br />
ROS, we generated an RNAi-mediated silencing tobacco line targeted at NtACS4 under the control of the<br />
DEX-inducible promoter. NtACS4i lines, which were more tolerant compared to wild-type, significantly<br />
resulted in the blockage of massive production of ROS and ethylene in second phase. We will investigate<br />
more physiological significance in the interaction between ROS and ethylene in both phases.<br />
DEVELOPMENT OF A SCREENING METHOD FOR R GENE FUNCTION IN WHEAT<br />
Remy Kronbak, Chang Yin, Mogens S. Hovmøller, Preben B. Holm, Per L. Gregersen<br />
88<br />
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