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A B S T R A C T B O O K – A B S T R A C T S O F T A L K S CHLOROPLAST DIFFERENTIATION BY SECRETORY PROTEIN Yasuo Niwa, Akiko Ogino, Hironori Kageshima, Shingo Goto, Hirokazu Kobayashi University of Shizuoka, Shizuoka, Japan E-mail: niwa@u-shizuoka-ken.ac.jp 29 X X I V S P P S C O N G R E S S 2 0 1 1 Session 5.Cell biology The plant hormones such as auxin and cytokinin can control plant cell de-differentiation and re-differentiation. It is possible to induce green callus from white callus by high concentration of cytokinin treatment. In this study, white callus induced from roots of Arabidopsis were used for mutant screening by activation tagging. Mutant candidates were selected as a greening phenotype from the white callus without high levels of cytokinin treatment. As a result, three candidates can be obtained and named ces101, 102, and 103. To identify the gene for ces102 phenotype, TAIL-PCR (thermal asymmetric interlaced-PCR) was performed. T-DNAs were found to be inserted into two locations in Arabidopsis genome. The expression level of two genes located near the insertion points was increased. From the results of phenotypic analysis by over-expression of candidate genes, the gene encoding 119 amino acids corresponds to ces102. According to the characteristics of the amino acid sequence, CES102 was expected to have a signal peptide. Localization analysis of GFP fusion protein, CES102-GFP could be detected at ER. From these results, plastid differentiation might be controlled through the secretory pathway.
Session 5.Cell biology A B S T R A C T B O O K – A B S T R A C T S O F T A L K S NETWORK MOTIFS LEADING TO PLANT NITRATE HOMEOSTASIS AND OSCILLATORY NITRATE ASSIMILATION Yongshun Huang, Ingunn W. Jolma, Tormod Drengstig, Peter Ruoff Centre for Organelle Research, University of Stavanger, Stavanger, Norway E-mail: peter.ruoff@uis.no Homeostasis is an essential property of all living systems. While themolecular details of homeostatic component processes such as uptake, storage,efflux and assimilation are becoming well understood our knowledge how theprocesses integrate and lead to robust homeostatic behavior in the presence ofenvironmental perturbations is still poor. Here we describe a complete set oftwo-component negative feedback motifs, which, based on thecontrol-engineering concept of integral feedback can maintain robusthomeostasis both at steady state and at oscillatory or pulsatile workingconditions. By using cytosolic nitrate homeostasis in plants as an example we demonstrate how these controller motifs integrate uptake, storage,efflux, and assimilation, and how switching between different controller typescan occur during remobilization of nitrate from the vacuole. 30 X X I V S P P S C O N G R E S S 2 0 1 1
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Page 3 and 4: Foreword eword ord A B S T R A C T
Page 5 and 6: Program of the Congress gram of the
Page 7 and 8: A B S T R A C T B O O K - P R O G R
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Page 11 and 12: Plenary session 1 A B S T R A C T B
Page 13 and 14: Plenary session 2 THE BIOLOGY OF AL
Page 15 and 16: Session 1. Biotechnology and synthe
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Page 19 and 20: Session 2. Systems biology and -omi
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Page 27 and 28: Pleanary session 4 A B S T R A C T
Page 29: Invited talk 4 A B S T R A C T B O
Page 33 and 34: Session 6.Organelle biology A B S T
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Page 39 and 40: Session 8.Signalling A B S T R A C
Page 41 and 42: Pleanary session 6 A B S T R A C T
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SCF = A B S T R A C T B O O K - A B
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A B S T R A C T B O O K - L I S T O
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Sponsors onsors ors A B S T R A C T
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