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A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S Microtubule (+)-end-associated protein SPR2/TOR1 from Arabidopsis is necessary for cortical microtubule array dynamics in cell elongation. Potato homolog of SPR2, designated as HIP2, interacts with Helper component proteinase of Potato virus A (HCpro of PVA). PVA belongs to the genus Potyvirus (family Potyviridae), the largest group of plant viruses. PotyviralHCpro is a multifunctional protein involved in virus movement within host and suppression of the fundamental antiviral defence system, RNA silencing. The aim of this study is to characterize potato HIP2 and explain function of the interaction between viral HCpro and microtubules in infected plants. Potato HIP2 complemented twisting phenotype of Arabidopsis spr2-mutant, confirming functional homology between HIP2 and SPR2. Transiently expressed HIP2 localized to microtubules in planta. In yeast two-hybrid system (YTHS) and bi-molecular fluorescence complementation (BiFC) analyses, HIP2 interacted with HCpro in yeast and plant cells, respectively. Importantly, the interaction took place during PVAinfection in leaves and aligned to cortical microtubules. HCpro domain required for HIP2-interaction was mapped in YTHS. Mutagenesis of the virus at this putative HIP2-interaction domain reduced virus accumulation in systemically infected leaves of potato. These data suggest the microtubulus-interaction via HIP2 might be beneficial for PVA. THE ROLE OF PEROXISOME-TARGETED NDR1/HIN1 LIKE (NHL) PROTEINS IN PATHOGEN DEFENCE Amr Kataya, Chimuka Mwaanga, Sigrun Reumann Centre for Organelle Research, University of Stavanger, Stavanger, Norway E-mail: amr.kataya@uis.no Peroxisomes are subcellular organelles that are traditionally known to be involved in processes like photorespiration, fatty acid β-oxidation and detoxification of reactive oxygen species. However, recent evidence indicates an important role of leaf peroxisomes also in defence against pathogens and herbivores. By proteome analyses and protein targeting prediction, we identified candidate defense-related proteins from the model plant Arabidopsis thaliana that are potentially targeted to peroxisomes. In particular, several yet unknown homologs of Arabidopsis NDR1 and tobacco HIN1 have been predicted. The full-length Arabidopsis cDNAs were fused to the reporter protein, enhanced yellow fluorescent protein (EYFP), and expressed transiently in plant cells. Peroxisome targeting was difficult to show in onion epidermal cells but convincingly demonstrated in tobacco protoplasts. The predicted peptides were validated as functional peroxisome targeting signals type 1 (PTS1). Real-time PCR has been established to investigate gene induction by defence hormones, elicitors, and bacterial pathogens. The NHL genes of interest are induced by salicylic acid but not jasmonic acid to different degree. All homologs were induced upon infection by virulent Pseudomonas syringaepv. Tomato DC3000, while only one homolog was induced by an avirulent strain carrying avrRpt2. The same gene was induced in wild-type plants by the bacterial elicitor, FLG22, but remained unaffected in plants carrying mutations in the flagellin receptor gene FLS2, suggesting that the defence protein is involved in basal PAMP triggered immunity (PTI). In homozygous knock-out mutants, significant differences in bacterial growth rates were observed compared to wild-type plants upon infection with Pst DC3000 (avrRpt2). Taken together, the data indicate for the first time that NDR1/HIN1 homologs are located in peroxisomes and play important roles in plant innate immunity. CHITOSAN AFFECTS GUARD CELL PHOTOSYNTHESIS AND MEMBRANE TRANSPORT Attila Ördög 1 , Barnabás Wodala 1 , Éva Hideg 2 , Ferhan Ayaydin 2 , Zsuzsanna Deák 2 , Ferenc Horvath 1 1 Department of Plant Biology, University of Szeged, Szeged, Hungary 2 Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary E-mail: horvathf@bio.u-szeged.hu Guard cells (GC) respond to the presence of microbes by narrowing stomatal pores following perception of microbe-associated molecular patterns, such as chitosan (CHT).It has been shown that CHT inhibits the blue light-induced stomatal opening and can trigger stomatal closure. These movements are related to the H + - ATPase activity in the GC plasma membrane, as it affects the transport of osmotically active solutes, such as the passive movement of K + via different sets of potassium channels, the activity of Cl - /H + symporters and 87 X X I V S P P S C O N G R E S S 2 0 1 1
A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S anion channels. The ATP for proton pumping is supplied mostly from mitochondrial respiration; however, a partial inhibition with DCMU implies a role of GC photosynthetic electron transport in the ATP supply. In order to investigate whether CHT affects the photosynthetic ATP production of GCs, the lightdependence of the photosynthetic electron transport rate of individual GCs was assayed. Vicia faba leaves were treated with CHT and chlorophyll fluorescence parameters of GCs were measured by Microscopy-PAM chlorophyll fluorometer.In addition, to test the possible effect of CHT on the activity of ion channels, Vicia GC protoplasts were investigated by patch clamp technique. Acknowledgements This work was supported by the Hungarian Scientific Research Fund (Grant. no. OTKA K 81471). DEMONSTRATION OF DIFFERENTIALLY REGULATED PROTEINS DURING THE INTERACTION BETWEEN PHYTOPHTHORA INFESTANS AND A TRANSGENIC POTATO EXPRESSING RPI-BLB1 FROM SOLANUM BULBOCASTANUM B. Colignon 1,2 , M. Raes 2 , E. Delaive 2 , M Dieu 2 , B .Watillon 1 , S. Mauro 1 1 Département Sciences du Vivant, Centre wallon de Recherches agronomiques, Gembloux, Belgium 2 Unité de Recherche en Biologie Cellulaire, Facultés Universitaires Notre-Dame de la Paix, Namur, Belgium E-mail: b.colignon@cra.wallonie.be Solanum tuberosum (cv Desirée) is susceptible to the attack of Phythophthora infestans Mont de bary. The Rpi-blb1 gene from Solanum Bulbocastanum, encoding a cytoplasmic NBS-LRR protein which recognizes Phytophthora effectors in the “gene for gene” model, confers resistance to the pathogen. We made a dynamic total leaf proteome comparison of the wild type and transgenic potato cultivars using a 2D DIGE strategy coupled to MS identification. Proteomic data revealed 33 and 21 proteins to be differentially expressed in transgenic and wild type challenged leaves respectively. Using principal component analysis we could clearly separate challenged and healthy leaf tissues. By using a systems biology approach, we could map some of the identified proteins into pathways involved in the interaction with Phytophthora infestans. SIGNIFICANCE OF FIRST BURST OF ROS AND ETHYLENE AS SIGNALING MOLECULES IN PATHOGEN-INDUCED THEIR BIPHASIC GENERATION Soo Jin Wi, Ky Young Park Department of Biology, Sunchon National University, Jeollanam-do, South Korea E-mail: plpm@sunchon.ac.kr We investigated the relationship between ROS and ethylene in response to biotic stress with the fungal pathogen using transgenic tobacco plants, in which ethylene biosynthesis or signaling or ROS production was impaired. It was observed that wild-type plants exhibited a gene-specific expression of NtACS members of ACC synthase, which were regulated in a time-dependent manner. Pathogen-induced expression of NADPH oxidase, RbohD and RbohF, and ROS accumulation at phase I were significantly suppressed in transgenic plants, in which ethylene biosynthesis and signaling were impaired. Biphasic ethylene production was also inhibited, especially at 1h, in stress-tolerant transgenic plants with impairment of RbohD and RbohF expression. The growth of Phytophthora, determined by expression of its 18s rRNA, was significantly reduced in transgenic plants with the impairment of ROS generation and ethylene biosynthesis and signaling pathway. To determine the physiological function of first burst of ethylene and ROS, we generated an RNAi-mediated silencing tobacco line targeted at NtACS4 under the control of the DEX-inducible promoter. NtACS4i lines, which were more tolerant compared to wild-type, significantly resulted in the blockage of massive production of ROS and ethylene in second phase. We will investigate more physiological significance in the interaction between ROS and ethylene in both phases. DEVELOPMENT OF A SCREENING METHOD FOR R GENE FUNCTION IN WHEAT Remy Kronbak, Chang Yin, Mogens S. Hovmøller, Preben B. Holm, Per L. Gregersen 88 X X I V S P P S C O N G R E S S 2 0 1 1
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Foreword eword ord A B S T R A C T
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Program of the Congress gram of the
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A B S T R A C T B O O K - P R O G R
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A B S T R A C T B O O K - P R O G R
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Plenary session 1 A B S T R A C T B
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Plenary session 2 THE BIOLOGY OF AL
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Session 1. Biotechnology and synthe
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Session 1. Biotechnology and synthe
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Session 2. Systems biology and -omi
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Session 2. Systems biology and -omi
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A B S T R A C T B O O K - A B S T R
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A B S T R A C T B O O K - A B S T R
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Pleanary session 4 A B S T R A C T
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Invited talk 4 A B S T R A C T B O
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Session 5.Cell biology A B S T R A
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Session 6.Organelle biology A B S T
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Session 6.Organelle biology A B S T
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Page 41 and 42: Pleanary session 6 A B S T R A C T
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Page 67 and 68: Sapporo Medical University, Sapporo
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Page 97 and 98: Sponsors onsors ors A B S T R A C T
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