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Methods in Anopheles Research - MR4

Methods in Anopheles Research - MR4

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Chapter 3 : Specific <strong>Anopheles</strong> Techniques3.1 Embryonic Techniques3.1.1 Micro<strong>in</strong>jection <strong>Methods</strong> for <strong>Anopheles</strong> EmbryosPage 7 of 8A: Younger embryos are difficult to <strong>in</strong>ject due to sensitivity to handl<strong>in</strong>g. Simply mov<strong>in</strong>g them early <strong>in</strong>development kills them.ReferencesGrossman GL, Rafferty CS, Clayton JR, Stevens TK, Mukabayire O, Benedict MQ (2001) Germl<strong>in</strong>etransformation of the malaria vector, <strong>Anopheles</strong> gambiae, with the piggyBac transposable element. InsectMol Biol 10:597-604Kim W et al. (2004) Ectopic expression of a cecrop<strong>in</strong> transgene <strong>in</strong> the human malaria vector mosquito<strong>Anopheles</strong> gambiae (Diptera: Culicidae): effects on susceptibility to Plasmodium. J Med Entomol 41:447-455

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