Methods in Anopheles Research - MR4

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Chapter 1 : Insectary Operation1.5 Insectary Manager ResponsibilitiesPage 1 of 61.5 Insectary Manager ResponsibilitiesMark Benedict“To provide authenticated, high-quality mosquito reagents, training and information to the researchcommunity of today and the future, in a timely and professional manner.”IntroductionWe begin this section with the mission statement for the MR4 vector activities. In order to accomplish this,the following list of responsibilities was developed as guidelines for the MR4 insectary manager. Whilethe details are specific for the MR4 vector activities at the CDC in Atlanta, it provides a useful guide forsupervisors employing a manager to oversee daily operations and in the development of a jobdescription. With little modification, this has served us well to describe the core activities of the manager.Insectary FacilitiesEnvironmentThe Vector Repository Manager (VRM) shall ensure that...1. Environmental conditions in insectaries are constantly maintained at 80 o F (±1.5 o F). Relative humiditywill be controlled to be in the range of 80% (± 10%) 365 days a year without interruption. Lighting iscontrolled such that a 30 minute sunrise and sunset occur, in between which times, the fluorescentlights will be on continually. The total darkness between the end of sunset and the beginning ofsunrise is 12 hours.2. The environmental conditions, except for lighting, are continually monitored by CDC maintenance staffand changes should be made to settings to achieve the above only after consultation and approval bythe VRM.3. Environmental conditions including lighting are continually and independently monitored by the MR4staff. This is achieved by sensors that are located in all three insectaries and capable of notifying MR4staff of conditions that are outside of the permissible range within 10 minutes regardless of whetherstaff are in the MR4 facility, at home, or traveling as necessary to ensure that at least one staff isaware of the problem.4. Pest insect control is continually performed to ensure essential absence primarily of ants andcockroaches. This is achieved in a way that no harm occurs to the insect colonies either directly or bycontamination with toxicants transported by pests. In the event that other pests are observed (e.g.mice), control is enacted as needed, but again with highest regard for the health of the repositoryinsects. Modifications of the facility are considered that physically reduce entry points, breeding sites,and harborages.5. Insect pest control around the perimeter of the building to reduce external sources is considered andexercised if needed.6. Neither CDC personnel, nor local municipalities conduct insect control in the vicinity of the insectaryfacilities.7. Properly operating mosquito traps or other killing devices operate continuously and are monitored forcatches in all insectaries. These should be capable of trapping primarily Anopheles, but also Aedesand Culex species.8. Cleanliness is maintained in all insectaries and support areas. While hospital cleanliness is neitherattainable nor necessary, a consistent effort should be made to improve the level of cleanliness. Inpart this will require labor, but use of materials and furnishings that do not rust and are easily cleanedwill be helpful. Only cleaning compounds that are non-toxic to the mosquitoes are used, but theseshould be used to reduce cleaning maintenance when possible. Moreover, the CDC maintenance
Chapter 1 : Insectary Operation1.5 Insectary Manager ResponsibilitiesPage 2 of 6staff is instructed to maintain the cleanliness of the floor and other areas within their responsibility. TheVRM is responsible for cleanliness but is not to become the custodian.9. Air filtration is installed and maintained properly to reduce the level of odors, fungi, dust, hair etc.Installing additional equipment or modifying existing equipment is considered to improve the airquality. Mold growing on mosquitoes and the insectary walls can be reduced by consistent attention toeliminating spores. Centralized UV sterilization of the air may be feasible.10. Ensure that documentation of the maintenance of the emergency generator is available and beingmaintained. Notify the PI in the event of any planned power outage.Infrastructure ImprovementsThe VRM shall ensure that...1. Sign-holders are installed that contain information about specific courses of action to take in the eventof various environmental anomalies. These will be located either near the alarms and/or by eachdoorway.2. Signage is current and attractively maintained.3. All infrastructure and environmental changes are consistent with the MR4 objectives. Furthermore,these changes are approved by all insectary users.Infrastructure MaintenanceThe VRM shall ensure that...1. Hallways are kept clear of trash, boxes, unused carts, old equipment etc.2. All lights function. The maintenance personnel should be notified in the event of lights burning out andother electrical problems.3. Timers are properly set and maintained.4. Hallway and insectary walls are kept clean and free of un-necessary notes, tape, scuffs, holes, tacksetc.Insectary SuppliesThe VRM shall ensure that...1. Consumables required for the operation of the insectary are maintained at sufficient levels thatshortages do not occur. The supply should be supplemented long before the need becomes critical.Allowance should be made for shipping delays and incorrect or incomplete orders.2. Establish minimum levels of supplies at which orders will be placed.3. Maintain inventory information sufficiently to ensure above.4. Consumables are safe, and have no characteristics that are an immediate threat to the mosquitostocks.5. Alternative consumables are considered for use. Materials that save time and/or money are soughtand tested.6. Maintain the cleanliness and order of the storage areas.7. Mosquito food and blood sources are safe and of an adequate amount to ensure that shortages do notoccur.
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INTRODUCTIONChapter 5 : Insecticide
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Chapter 9 : Obtaining Live Material
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Chapter 9 : Obtaining Live Material
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Methods in Anopheles Research - MR4

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