Methods in Anopheles Research - MR4

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Chapter 1 : Insectary Operation1.1 Equipping and Operating an InsectaryPage 3 of 4such alarms. This system has saved stocks numerous times and has used pagers, cell phones, andBlackberries for notification.Comfort of personnelThis should be strongly considered during the design of the insectary. Few enjoy working in a hot humidinsectary, and this can be reduced by subdividing adult and larval holding areas, using lower humidity orrelying on incubators.FurnitureRust-proof metal, fiberglass or plastic furnishings are preferable. Shelving should be easily adjustable andstand-alone units should be equipped with wheels. As is discussed in the chapter on cleanliness andgeneral maintenance, one’s ability to clean equipment – and beneath it – is essential. All furnishings mustbe suitable for being wet frequently.Supplies and culture equipmentFollowing is a list of typical supplies needed to equip a small insectary. The sources for much of thismaterial will be different depending on your location and many substitutions are possible. Though somesources are local, the URLs will provide information to give you an idea of what is described. We indicateFisher Scientific as a source for many items, but these products are widely available. Many practices andinnovative devices are found around the world, so collect good ideas and device solutions in all theinsectaries that you visit.Mosquito Rearing Equipment/Supplies8 oz. white plastic containers (for pupae)-approx 250ml, used in food serviceSource exampleModelexampleURLUS Plastics 81134 www.usplastic.comBugDorm1 adult cage (option 1)MegaViewScienceDP1000www.megaview.com.twwww.bioquip.com12x12x12 Metal cage ( option 2) BioQuip 1450B www.bioquip.comLarval rearing trays BioQuip 1426B www.bioquip.comPlexiglas covers for traysFabricate locally2 ml amber latex pipette bulbs FisherScientific S32324 www.fishersci.comPlastic disposable pipettes (trim end,attach bulb and use as a pupae picker)Stainless steel mesh strainer (to filterlarvae and pupae)FisherScientific 13-711-7 www.fishersci.comLocal source SDM-05 www.atlantafixture.comTubes for mixing yeast e.g. 15 mldisposableFisherScientific05-538-51 www.fishersci.com10 ml pipettes FisherScientific13-678-12Ewww.fishersci.comSucrose (to make 10% sugar solution foradults)Local sourcewww.fishersci.comLarge cotton balls FisherScientific 07-886 www.fishersci.comcolored tape (to label and discriminatestocks-choose 1 color per stock)FisherScientific15-901-15(colorcode)www.fishersci.com
Chapter 1 : Insectary Operation1.1 Equipping and Operating an InsectaryPage 4 of 4Larval diet e.g. Drs. Foster and Smith KoiStaple DietDrs. Foster andSmithKoiStapleDietwww.drsfostersmith.com‘dash, pinch, smidgen’ stainless steelmeasuring spoonsLocal sourceMouth aspirator 1 John Hock Co. 412 www.johnwhockco.comFeather-tip forceps Bioquip 4748 www.bioquip.com2 liter clear plastic pitchers with volumemarkingsLocal sourceFilter paper sheetsFisherScientific09-803-5Ewww.fishersci.comQorpak tubes or similar Qorpak 3891P www.qorpak.com500 ml wash bottles FisherScientific02-897-11 www.fishersci.comWaterproof felt tip markers e.g. ‘Sharpie’ Local source 13-379-1 http://new.fishersci.comTable 1.1.1. Some useful insectary supplies and manufacturers.Common Entomological supply sources:• John Hock Company www.johnwhockco.com• Watkins and Doncaster www.watdon.com• BioQuip www.bioquip.com• Educational Science Co. www.educationalscience.com• MegaView www.megaview.com.twReferencesBenedict MQ (1997) Care and maintenance of anopheline mosquito colonies. In: Crampton JM, BeardCB, Louis C (eds) The Molecular Biology of Insect Disease Vectors. Chapman & Hall, New York, pp 2-12Benedict MQ (2003) Arthropod Containment Guidelines. Vector Borne and Zoonotic Diseases 3:63-98Dame DA, Haile DG, Lofgren CS, Bailey DL, Munroe WL (1978) Improved Rearing Techniques for LarvalAnopheles albimanus : Use of Dried Mosquito Eggs and Electric Heating Tapes. Mosq. News 38:68-74Gerberg EJ, Barnard DR, Ward RA (1994) Manual for mosquito rearing and experimental techniques,revised edn. American Mosquito Control Association, Inc., Lake Charles1 An aspirator can be constructed from a section of rigid transparent plastic tubing 20 cm long with aninside diameter of about 15 mm. One end of the tube is covered with fine cloth netting or metal gauze andthen inserted into a piece of rubber hose/tubing 50-60 cm)
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INTRODUCTIONChapter 5 : Insecticide
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Methods in Anopheles Research - MR4

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