Methods in Anopheles Research - MR4

Chapter 2 : Anopheles Laboratory Biology and Culture2.4 Anopheles Culture2.4.5 Estimating the Number of Eggs and LarvaePage 3 of 4• Finally, one could pour the concentrated larvae into a known volume of water and mix them untilthey were uniformly dispersed (Gerberg et al. 1968, 1994). From the known volume 20-25 1mlaliquots are removed so as to create an estimate of larvae per ml from which aliquots can bedispensed into new rearing pans.ReferencesCarron A, Duchet C, Gaven B, Lagneau C (2003) An easy field method for estimating the abundance ofculicid larval instars. J Am Mosq Control Assoc 19:353-360Dame DA, Haile DG, Lofgren CS, Bailey DL, Munroe WL (1978) Improved Rearing Techniques for LarvalAnopheles albimanus : Use of Dried Mosquito Eggs and Electric Heating Tapes. Mosq. News 38:68-74Gerberg EJ, Barnard DR, Ward RA (1994) Manual for mosquito rearing and experimental techniques,revised ed. American Mosquito Control Association, Inc., Lake CharlesGerberg EJ, Gentry JW, Diven LH (1968) Mass rearing of Anopheles stephensi Liston. Mosq. News 28342-346Rutledge LC, Sofield RK, Piper GN (1976) Rapid counting methods for mosquito larvae. Mosq. News 36537-539