Methods in Anopheles Research - MR4

Chapter 3 : Specific Anopheles Techniques3.1 Embryonic Techniques3.1.2 Anopheles Embryo FixationPage 3 of 4point.17. Let stand 15-20 minutes.18. Remove as much of both phases as possible. Rinse with 5 ml methanol twice removing anyexcess liquid, then add 5 ml fresh methanol.19. At this point, the embryos can be stored at -20 o C in methanol for several months.20. The endochorion must be manually peeled away using fine needles and double stick tape beforestaining.ReferencesGoltsev Y, Hsiong W, Lanzaro G, Levine M (2004) Different combinations of gap repressors for commonstripes in Anopheles and Drosophila embryos. Dev Biol 275:435-446Yoder JH, Carroll SB (2006) The evolution of abdominal reduction and the recent origin of distinctAbdominal-B transcript classes in Diptera. Evol Dev 8:241-251