Methods in Anopheles Research - MR4

More documents

Recommendations

Info

Chapter 3 : Specific Anopheles Techniques3.7 Anopheles Mating3.7.2 Forced CopulationPage 3 of 6Figure 3.7.2.3. Holding male with vacuumpressure, gently stab the male through thethorax taking care to not crush the head.Figure 3.7.2.4. Properly pinned male.6. Place one container of 10 females into the anesthetizing chamber and leave it for 6-10 seconds(depending on the species and strength of ether).7. Watch the females closely, and once they have all fallen from the sides, remove them from thechamber. Do not leave females in the ether too long or they will not recover from the treatment.8. Gently disperse the females onto a piece of filter paper and position ventral side up. Take a mountedmale, pinch off the head and hind-tarsi (Figure 3.7.2.5), and then gently stroke the abdomen of themale over the female’s abdomen to stimulate the claspers to open (Figure 3.7.2.6).9. Place the male at a 45-90° angle venter-to-venter with the female until the male clasps the female(Figure 3.7.2.7). Leave clasped for 1-2 seconds then pick up both using the male on the pin. If matedsuccessfully, they will remain attached for several seconds. 2 Successful mating has usually occurredif they remain attached for 3-5 seconds (Figure 3.7.2.8).10. Place male and female together into a new cage to allow female to recover from the anesthesia.11. Ensure that the females are recovering from the anesthesia by gently blowing into the recovery cage.If females are not waking up within 10 minutes, anesthetize more lightly.2 It is possible to reuse males to mate more than one female.
Chapter 3 : Specific Anopheles Techniques3.7 Anopheles Mating3.7.2 Forced CopulationPage 4 of 6Figure 3.7.2.5 Male with head and legs removed.Figure 3.7.2.6 Positioning of the male prior tostroking the females abdomenFigure 3.7.2.7 Pairing of male and female, note themale is near a 90°angle to the femaleFigure 3.7.2.8 If pairing is successful, you areable to lift the female using the male.
Page 1:
\Methods inAnopheles ResearchSecond
Page 8 and 9:
Preface to Second EditionWhen Mark
Page 10 and 11:
Chapter 1 : Insectary Operation1.1
Page 12 and 13:
Page 14 and 15:
Page 16 and 17:
Page 19 and 20:
Page 21 and 22:
Page 23 and 24:
Page 25 and 26:
Page 27 and 28:
Page 29 and 30:
Page 31 and 32:
Page 33 and 34:
Page 35 and 36:
Chapter 2 : Anopheles Laboratory Bi
Page 37 and 38:
Page 39 and 40:
Page 41 and 42:
Page 43 and 44:
Page 45 and 46:
Page 47 and 48:
Page 49 and 50:
Page 51 and 52:
Page 53 and 54:
Page 55 and 56:
Page 57 and 58:
Page 59 and 60:
Page 61 and 62:
Page 63 and 64:
Page 65 and 66:
Page 67 and 68:
Page 69 and 70:
Page 71 and 72:
Page 73 and 74:
Page 75 and 76:
Page 77 and 78:
Page 79 and 80:
Page 81 and 82:
Page 83 and 84:
Page 85 and 86:
Page 87 and 88:
Page 89 and 90:
Page 91 and 92: Chapter 2 : Anopheles Laboratory Bi
Page 93 and 94: Chapter 2 : Anopheles Biology Labor
Page 107 and 108: Chapter 3 : Specific Anopheles Tech
Page 115 and 116: 5. Place eggs in a new Petri dish c
Page 141: Chapter 3 : Specific Anopheles Tech
Page 159 and 160: Chapter 4 : Stock Authentication4.1
Page 169 and 170: Figure 4.2.3.1 Lane 1 1kb ladder, l
Page 171 and 172: Chapter 5 : Insecticide Resistance
Page 177 and 178: INTRODUCTIONChapter 5 : Insecticide
Page 179 and 180: Chapter 5 : Insecticide Resistance5
Page 193 and 194:
Chapter 5 : Insecticide Resistance5
Page 195 and 196:
Page 197 and 198:
Page 199 and 200:
Page 201 and 202:
Chapter 5 : Insecticide Resistance
Page 203 and 204:
Page 205 and 206:
Page 207 and 208:
Page 209 and 210:
Page 211 and 212:
Page 213 and 214:
Page 215 and 216:
Page 217 and 218:
Page 219 and 220:
Page 221 and 222:
Page 223 and 224:
Page 225 and 226:
Chapter 6 : Dissection Techniques6.
Page 227 and 228:
Figure 6.2.1. Larval mosquito midgu
Page 229 and 230:
Page 231 and 232:
Page 233 and 234:
Page 235 and 236:
Page 237 and 238:
Page 239 and 240:
Page 241 and 242:
Page 243 and 244:
Page 245 and 246:
Page 247 and 248:
Page 249 and 250:
Page 251 and 252:
Page 253 and 254:
Chapter 7 : Taxonomy and Systematic
Page 255 and 256:
Page 257 and 258:
Page 259 and 260:
Page 261 and 262:
Page 263 and 264:
Page 265 and 266:
Collection NumberCountryNumber Le P
Page 267 and 268:
MOSQUITO BITING COLLECTION RECORDCo
Page 269 and 270:
Page 271 and 272:
Page 273 and 274:
Page 275 and 276:
Page 277 and 278:
Page 279 and 280:
Chapter 8 : Field Techniques8.1 Mol
Page 281 and 282:
Page 283 and 284:
Chapter 8 : Field Techniques8.2 Pla
Page 285 and 286:
Page 287 and 288:
Page 289 and 290:
Page 291 and 292:
Page 293 and 294:
Page 295 and 296:
Page 297 and 298:
Page 299 and 300:
Chapter 8 : Field Techniques8.4 Spe
Page 301 and 302:
Page 303 and 304:
Page 305 and 306:
Page 307 and 308:
Page 309 and 310:
Page 311 and 312:
Page 313 and 314:
Page 315 and 316:
Chapter 8 : Field Techniques8.5 Rea
Page 317 and 318:
Page 319 and 320:
Page 321 and 322:
Page 323 and 324:
Page 325 and 326:
20Chapter 8 : Field Techniques8.5 R
Page 327 and 328:
Page 329 and 330:
Page 331 and 332:
8070Mutant (S1014)Chapter 8 : Field
Page 333 and 334:
Page 335 and 336:
AChapter 8 : Field Techniques8.5 Re
Page 337 and 338:
Page 339 and 340:
Page 341 and 342:
Chapter 9 : Obtaining Live Material
Page 343:
Chapter 9 : Obtaining Live Material
show all

Methods in Anopheles Research - MR4

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?