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Methods in Anopheles Research - MR4

Methods in Anopheles Research - MR4

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Chapter 1 : Insectary Operation1.3 Schedul<strong>in</strong>g and Regulat<strong>in</strong>g Your Work LoadPage 3 of 4Suggested Schedule 2: a three-week cycle beg<strong>in</strong>n<strong>in</strong>g on MondayThis follows the schedule above, but shifted. This schedule will probably result <strong>in</strong> pupation over theweekend so it may not be as convenient.Monday: Blood-feed adult females.Tuesday: No attention required.Wednesday: No attention required.Thursday: Collect eggs.Friday: Remove the egg dish and bleach the eggs.Saturday: Hatch larvae.Sunday: No attention required.Monday: Feed and split/th<strong>in</strong> larvae.Tuesday through Thursday: Th<strong>in</strong> and feed pans as needed.Friday through Sunday: Collect pupae or adults and feed larvae every day.Monday follow<strong>in</strong>g week: Blood-feed to re<strong>in</strong>itiate the cycle.Both schedules are laid out <strong>in</strong> calendar form <strong>in</strong> Table 1.3.1.Mon Tue Wed Thu Fri Sat SunWeek 1Schedule 1 blood eggSchedule 2 blood eggdish bleach hatchMon Tue Wed Thu Fri Sat SunWeek 2Schedule 1 bleach hatch split/th<strong>in</strong> split/th<strong>in</strong>Schedule 2 split/th<strong>in</strong> split/th<strong>in</strong> feed feed feed feedMon Tue Wed Thu Fri Sat SunWeek 3Schedule 1 feed feed Feed pupation pupation pupation pupationSchedule 2 pupae pupae Pupae pupaeMon Tue Wed Thu Fri Sat SunWeek 4Schedule 1bloodSchedule 2 blood eggdish bleach hatchTable 1.3.1. Calendar layout of two schedules as described above.

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