Methods in Anopheles Research - MR4

Chapter 3 : Specific Anopheles Techniques3.4 Mosquito AnesthesiaPage 3 of 4should be performed in an area with good ventilation, preferably a chemical fume hood or other form oflocal exhaust enclosure.Employees using this chemical should be trained to recognize the acute and chronic health effectsassociated with an over-exposure that can occur by inhalation, absorption through the skin and byingestion. Selection of gloves is of particular importance since permeation of some nitrile gloves canoccur within as little as 3 minutes. Contact the glove manufacturer for specific selectionrecommendations. Employees should always be informed of glove limitations and trained accordinglyeven for incidental use. Chloroform should not be stored with caustics. Review the MSDS for additionalsafe handling, storage and disposal information.AcknowledgmentsThanks to Paul Vinson and Cheryl Connell of the CDC Office of Health and Safety for safety advice.ReferencesKramer LD, Presser SB, Houk EJ, Hardy JL (1990) Effect of the anesthetizing agent triethylamine onwestern equine encephalomyelitis and St. Louis encephalitis viral titers in mosquitoes (Diptera:Culicidae). J Med Entomol 27:1008-1010McCuiston LJ, White DJ (1976) Laboratory colonization of Aedes sollicitans (Walker) with a review of thetechnique of induced copulation. Proc N.J. Mosq Control Assoc.:164-175