Methods in Anopheles Research - MR4

Chapter 5 : Insecticide Resistance5.1 Insecticide Resistance Bioassays5.1.3 Guidelines for Evaluating Insecticide Resistance in Vectors using the CDC Bottle BioassayPage 5 of 24insecticides have been used for 2–3 years without degradation of activity. It is recommended to take thestock solutions out of the refrigerator at least 1 hour before running the bioassay to allow them to come toroom temperature before use. The stock solution should be gently swirled before use to mix it.Table 5.1.3.2: Quantities of technical grade insecticide required for preparation of differentvolumes of stock solution.InsecticideWeight (mg) of technical gradeinsecticide needed per volume ofstock solution (Anopheles)Weight (mg) of technical gradeinsecticide needed per volume ofstock solution (Aedes)100 ml 500 ml 1,000 ml 100 ml 500 ml 1,000 mlBendiocarb 1.25 6.25 12.5 1.25 6.25 12.5Cyfluthrin 1.25 6.25 12.5 1 5 10Cypermethrin 1.25 6.25 12.5 1 5 10DDT 10 50 100 7.5 37.5 75Deltamethrin 1.25 6.25 12.5 1 5 10Fenitrothion 5 25 50 5 25 50Lambdcyhalothrin 1.25 6.25 12.5 1 5 10Malathion 5 25 50 5 25 50Permethrin 2.15 10.75 21.5 1.5 7.5 15Pirimiphos-methyl 2 10 20