Methods in Anopheles Research - MR4

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Chapter 8 : Field Techniques8.5 Real-Time PCR Assays8.5.1 Vector Population Monitoring Tool using Real-Time PCRPage 3 of 4Assay ControlsThe following table details the control genotypes currently available from the Malaria Research andReference Reagent Resource Center (MR4) www.mr4.org. We have also created additional plasmidand whole genome amplified controls for these assays available from the authors on request.Anopheles gambiae s.l. species identification (Section 8.5.1.1)MRA-495 Anopheles arabiensis Genomic DNA from An. arabiensis KGBMRA-142 Anopheles gambiae Genomic DNA from An. gambiae, G3 strainMRA-761G Anopheles quadriannulatus Genomic DNA from An. quadriannulatus, SKUQUA strainDetection of infection with Plasmodium parasites (Section 8.5.1.2)MRA-102G Plasmodium falciparum Genomic DNA from P. falciparum 3D7MRA-341G Plasmodium vivax Genomic DNA from P. vivax ONGDetection of knock-down resistance (kdr) mutations (Section 8.5.1.3)MRA-762 Anopheles gambiae KISUMU Wild-type susceptible homozygous controlMRA-334 Anopheles gambiae RSP Kdr L1014S (Kdr-e) homozygousDetection of insensitive acetylcholinesterase (iAChE) (Section 8.5.1.4)MRA-762 Anopheles gambiae KISUMU Wild-type susceptible homozygous controlMRA-913 Anopheles gambiae AKRON Wild-type resistant controlDetection of resistance to dieldrin (rdl) (Section 8.5.1.5)MRA-115 Anopheles gambiae IN22C+ Wild-type resistant homozygous control (containedwithin IN22C+ strain, only c+ individuals)MRA-762 Anopheles gambiae KISUMU Wild-type susceptible homozygous controlMRA-495 Anopheles arabiensis KGB Wild-type susceptible homozygous controlMRA-764 Anopheles arabiensis SENN Wild-type resistant homozygous controlAuthors also have the following plasmid controls available:Wild-type (susceptible homozygous control), Kdr L1014S (kdr-e) homozygous and Kdr L1014F (kdr-w)homozygousWild-type (susceptible homozygous control), and the G119S homozygous
Chapter 8 : Field Techniques8.5 Real-Time PCR Assays8.5.1 Vector Population Monitoring Tool using Real-Time PCRPage 4 of 4Authors AffiliationsChris Bass, Martin Williamson, and Lin FieldAddress: Insect Molecular Biology Group, Department of Biological Chemistry, Rothamsted Research,Harpenden, AL5 2JQ, UKEmail:Chris Bass - chris.bass@bbsrc.ac.uk;Martin S Williamson - martin.williamson@bbsrc.ac.uk;Linda M Field - lin.field@bbsrc.ac.ukHilary Ranson, Martin DonnellyAddress: Vector Group, Liverpool School of TropicalMedicine, Pembroke Place, Liverpool L35QA, UKEmail:Martin J Donnelly - m.j.donnelly@liverpool.ac.uk;Hilary Ranson - Hranson@liverpool.ac.ukJohn VontasAddress: Laboratory of Pesticide Science, Agricultural University of Athens, Iera Odos 75, 118 55,Votanikos, Athens, GreeceEmail:John Vontas - vontas@aua.gr
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Preface to Second EditionWhen Mark
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Chapter 1 : Insectary Operation1.1
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Methods in Anopheles Research - MR4

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