Methods in Anopheles Research - MR4

Chapter 1 : Insectary Operation1.5 Insectary Manager ResponsibilitiesPage 3 of 6OtherThe VRM shall insure that...1. Office supplies necessary for the timely shipment and documentation of MR4 reagents is ensured.2. Materials to produce documentation for MR4 reagents are of high quality and of adequate amounts.3. Shipping materials are of good supply, quality, and suitability.4. Computer consumables such as CD/Rs, diskettes, paper etc. is of an adequate supply to producedocumentation, file archives, communication etc.Mosquito AuthenticationThe VRM shall ensure that...1. Only authenticated materials are shipped from and maintained by the MR4.2. Authentication methods are developed that are reproducible with reasonable ease both within therepository and by requesters.3. Materials required for authentication are protected from accidental contamination or loss and can beproduced on demand using independent means.4. Documentation is sufficient to enable requesters to authenticate materials independently.Preservation and ProductionThe VRM shall ensure that...1. Levels of all MR4 stocks are sufficient to ensure a constant supply of material for all MR4 activities.2. Non-MR4 personnel who maintain MR4 stocks are informed about the requirements for theenvironment in the insectary and procedures to follow to ensure that the stocks are maintained withoutcontamination or loss. This must be done without imposing upon them or requiring significantalteration of the existing procedures.3. No MR4 stocks become contaminated or lost. This is very important.4. Sufficient duplication of stocks is implemented to ensure an independent supply that providesinsurance against accidental loss. This may be in the form of on-site maintenance in separatefacilities, or a backup stock in another laboratory from whom the material could be obtained ifnecessary that would notify the VRM in the event of loss. Records of recipients of stocks should bereferred to as a final source of stocks.5. DNAs of stocks are prepared as proposed and distributed to the ATCC and additional backup stocksare maintained at the CDC.6. Sentinel adults are monitored for unusually reduced life span.7. The PI is notified promptly by voice and e-mail in the event of any stock contamination, reduction insupply, or unusual culture conditions.8. Improvements to culture methods are considered if these can save time and/or money.9. A current log is available on the web describing the condition of the stocks at all times including allauthentication.DistributionThe VRM shall ensure that...1. Shipments of mosquitoes are made at first availability of the requested material.2. Contents of shipments are correct, contain appropriate documentation, and are properly packaged.