Methods in Anopheles Research - MR4

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Preface to Second EditionWhen Mark Benedict and the MR4 Vector Activity Team launched the first edition of Methods inAnopheles Research in 2007, they never imagined its enormous success. This manual has becomeone of the most accessed items in the MR4 Vector Activity website, providing practical techniques to theintrepid researcher tasked with the “dicey” activity of rearing and investigating anopheline mosquitoes. Ithas become a valuable resource not only for the scientist conducting basic research but also for publichealth entomologist from malaria endemic countries and members of the vector control industry.The first edition of Methods in Anopheles Research was “the latest incarnation” of training materialsthat Mark had developed for his personnel over many years. In the preface of that version, he invitedother researchers to contribute protocols and to make corrections. And they did. Several minor revisionswere made and a few new techniques were added periodically over the following couple of years. As themanual became better known, the number of suggested additions increased. Because the new materialdid not fit well in the original chapters, we realized that we would need to make more than minor changesand could no longer consider it the same edition. This second edition of the Methods in AnophelesResearch reflects those modifications. Many of the previous chapters remain much the same, new oneshave been added to accommodate the new procedures and a few have been rearranged. It still remainsa work in progress and as Mark requested in the first edition, and we continue to urge researchers tocritique and to contribute.We thank all the people in the last several years that have made comments and suggestions and areespecially grateful to those who offered their techniques including the following:Claudia AliagaFrancis AtieliMelissa AveryChris BassJames BecnelNora BesanskyDmitri BoudkoWilliam BrogdonAdeline ChanAnthony CornelMartin DonnellyLin FieldChristen FornadelRalph HarbachClare HolleleyTheresa HowardChristian KaufmannRebekah KentMarc KlowdenLizette KoekemoerGreg LanzaroFrédéric LardeuxPaul LinserYvonne LintonJohn MorganUlrike MunderlohMarco NeiraK.R. Ng’habiDouglas NorrisHilary RansonRosenka TajerinaLeslie VanEkerisJohn VontasBradley WhiteElien WilkinsMartin WilliamsonRobert Wirtz
I would like to give special recognition to the invaluable contributions of Paul Howell and Alice Sutcliffe,whose diligence and persistence made this second edition possible.Finally I would thank all the users who have made this such a popular download and I hope that themanual remains a valuable resource for those working to reduce malaria and other vector-bornediseases.Sincerely,Ellen M. Dotson, Principal Investigator for MR4 Vector Activity, CDC Atlanta GA, USAApril, 2011
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Chapter 9 : Obtaining Live Material
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Chapter 9 : Obtaining Live Material
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