Methods in Anopheles Research - MR4

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Chapter 5 : Insecticide Resistance Monitoring5.1 Insecticide Resistance Bioassays5.1.1 Larval Insecticide Resistance AssaysPage 3 of 49. Determine mortality at fixed time intervals (Figure 5.1.1.3). It is often helpful to sink dead larvae bystirring and poking. Affected larvae are usually unable to return to the surface. 4Figure 5.1.1.1. L4 larvae in a dedicated treatmentcup. Most of the water has been removed using aplastic pipette.Figure 5.1.1.2. 200ml of a 1ppm dieldrin solutionwith approximately 200 L4 larvae. Since this is a1 hour treatment, no food has been added.4 There is often excessive concern that insecticides used for treating larvae will kill susceptible stocks inthe insectary due to carryover e.g. on trays, gloves, larvae. While this is possible and should beconsidered, calculation of realistic amounts of insecticide contamination can show that carryover amountsare usually too low to cause mortality even among susceptible individuals.
Chapter 5 : Insecticide Resistance Monitoring5.1 Insecticide Resistance Bioassays5.1.1 Larval Insecticide Resistance AssaysPage 4 of 4Figure 5.1.1.3. 2 hours post treatment, the pan on the left contains the resistant population which isevenly dispersed throughout the container while the pan on the right contains the susceptible control.Note the clumped L4 larvae on the bottom of the pan.ReferencesFrench WL, Kitzmiller JB (1963) Time in concentration: a simple technique for the accurate detection ofresistance to insecticides in mosquito larvae. In. World Health Organization, Geneva, WHO/MAL401
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Preface to Second EditionWhen Mark
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Chapter 1 : Insectary Operation1.1
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Chapter 2 : Anopheles Laboratory Bi
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Chapter 9 : Obtaining Live Material
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Chapter 9 : Obtaining Live Material
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Methods in Anopheles Research - MR4

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