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Methods in Anopheles Research - MR4

Methods in Anopheles Research - MR4

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Chapter 2 : <strong>Anopheles</strong> Biology Laboratory and Culture2.5 Basic <strong>Anopheles</strong> Mendelian GeneticsPage 5 of 8Sex l<strong>in</strong>kageProblem 2: Aga<strong>in</strong>, use a Punnett Square to illustrate the pattern. This example uses the sex-l<strong>in</strong>kedmarker white (w) which is located on the X chromosome (Benedict et al. 1996). The w+ allele is fullydom<strong>in</strong>ant over the w allele. The cross is between a heterozygous female and a hemizygous male whocarries the w+ allele. Fill <strong>in</strong> the pheno- and genotypes <strong>in</strong>clud<strong>in</strong>g sex.Female Gametes2a. w+ proportion = ……………. 2b. w proportion = …………….2c. w+ proportion =2e. w+ / w+ proportion =2e. w+ / w proportion =Male Gametes…………….2d. Y proportion =…………….……………………Sex = ………2e. w+ Y proportion =…………………Sex = …………………………..Sex = ………2e. w Y proportion =………………..Sex = ………Note that among the progeny, females and males occur at different frequencies <strong>in</strong> each eye-colorphenotypic class.Determ<strong>in</strong>e the genotypic frequencies2i. w+ / w female ____________2j. w / w female ____________2k. w+ / w+ female ____________2l. w / Y (male) ____________2m. w+ / Y (male) ____________Determ<strong>in</strong>e the phenotypic frequencies2n. w+ female ____________2o. w female ____________2p. w+ male ____________2q. w male ____________

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