Methods in Anopheles Research - MR4

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Chapter 3 : Specific Anopheles Techniques3.7 Anopheles Mating3.7.1 Mating : General ConsiderationsPage 1 of 23.7 Anopheles Mating3.7.1 Mating : General considerationsPaul HowellIntroductionMating in most anopheline mosquitoes occurs during the early evening and is believed to occur primarilyin swarms. Anopheles male mosquitoes aggregate just before dusk and commence swarming at theonset of sunset. Swarming males use their erect antennal fibrillae to detect a nearby female mosquito’swing beat frequencies (Nijhout and Sheffield 1979; Ikeshoji et al. 1985; Leemingsawat 1989; Clements1992). In Toxorhynchites it was found that males will actually harmonize their wing beat with females asthey near, possibly as a form of species recognition, before mating commences (Gibson and Russell2006). In many species, copulation is initiated in flight with males and females meeting within the swarms(Clements 1992). Once a male anopheline has grasped a receptive female, it reorients itself so it is in theventer-to-venter position allowing the reproductive organs to meet. After coitus commences, the malemoves into an end-to-end position with the female as the pair falls (Charlwood and Jones 1979).Copulation may continue for a short period of time after alighting, but in most genera it is a very quickprocess which ceases before the pair reaches the ground.Newly emerged anophelines are not sexually mature. Male mosquitoes require about 24 hours beforetheir terminalia have rotated and their fibrillae are mature enough to become erect and detect females(Clements 1992). Female mosquitoes, however, typically need 48-72 hours before they become receptiveto males - usually prior to blood feeding in the wild. Anopheles males can mate several times, but femalesbecome refractory to re-insemination and re-mating is rare (Villarreal et al. 1994; Yuval and Fritz 1994). InAn. culicifacies, it was found that a proportion of colonized females had multiple inseminations, but thiswas attributed to the laboratory setting and not a natural behavior (Mahmood and Reisen 1980).In the laboratory, it is often not feasible to maintain a colony in a large enough cage to promote naturalswarming behavior. Instead, selection of a stenogamous colony - one that breeds in a small cage - isperformed. During colonization, only a proportion of individuals will respond to the novel environment, anda genetic ‘bottleneck’ occurs - loss of heterozygosity. Norris et al. showed that even a newly colonizedstrain has an extreme loss of heterozygosity compared to field samples from the same area (Norris et al.2001). Often, fixation of particular alleles is a quick inevitable process and cannot be remedied withoutthe introduction of new field material.Although little is known about cues that are needed to stimulate mating within the laboratory, someexperiments have been done to develop methods to improve colony mating. The addition of a simulatedsunrise and sunset has been shown to have a positive effect on colonization efforts – presumably due toimproved mating (Charlwood and Jones 1980; Panicker and Bai 1980). There are also reports ofresearchers utilizing a low watt colored light prior to the dark period to stimulate mating (Pan et al. 1982;Villarreal et al. 1998). Cage size also has an impact on the success of a colony. Some species will notmate within a small cage due to some unknown parameter, such as eurygamy, and require larger cagesto complete mating (Pan et al. 1982; Marchand 1985). Marchand (1985) and Peloquin (1988) utilized anartificial sky and horizon to promote mating.ReferencesCharlwood JD, Jones MDR (1979) Mating behaviour in the mosquito, Anopheles gambiae s.l. I. closerange and contact behaviour. Phys Entomol 4:111-120Charlwood JD, Jones MDR (1980) Mating in the mosquito, Anopheles gambiae s.l. II. Swarmingbehaviour. Phys Entomol 5:315-320
Chapter 3 : Specific Anopheles Techniques3.7 Anopheles Mating3.7.1 Mating : General ConsiderationsPage 2 of 2Clements AN (1992) The Biology of Mosquitoes: Development, Nutrition and Reproduction. Chapman &Hall, LondonGibson G, Russell I (2006) Flying in tune: sexual recognition in mosquitoes. Curr Biol 16:1311-1316Ikeshoji R, Sakakihara M, Reisen WK (1985) Removal sampling of male mosquitoes from fieldpopulations by sound-trapping. Jpn J Sanit Zool 36:197-203Leemingsawat S (1989) Field trials of different traps for malaria vectors and epidemiologicalinvestigations at a foot-hill basin in Kanchanaburi, Thailand. Jpn J Sanit Zool 40:171-179Mahmood F, Reisen WK (1980) Anopheles culicifacies: the occurrence of multiple inseminations underlaboratory conditions. Entomol Exp Applic 27:69-76Marchand RP (1985) A new cage for observing mating behavior of wild Anopheles gambiae in thelaboratory. J Am Mosq Control Assoc 1:234-236Nijhout HF, Sheffield HG (1979) Antennal hair erection in male mosquitoes: a new mechanical effector ininsects. Science 206:595-596Norris DE, Shurtleff AC, Toure YT, Lanzaro GC (2001) Microsatellite DNA polymorphism andheterozygosity among field and laboratory populations of Anopheles gambiae ss (Diptera: Culicidae). JMed Entomol 38:336-340Pan JF, Yu TR, Zhu HK (1982) Study on laboratory breeding of Anopheles balabacensis balabacensisBaisas. WHO/MAL/82.989Panicker KN, Bai MG (1980) A note on laboratory colonization of Anopheles subpictus Grassi. Indian JMed Res 72:53-54Peloquin JJ, Asman SM (1988) Use of a modified Marchand cage to study mating and swarm behavior inCulex tarsalis, with reference to colonization. J Am Mosq Control Assoc 4:516-519Villarreal C, Arredondo-Jimenez JI, Rodriguez MH, Ulloa A (1998) Colonization of Anophelespseudopunctipennis from Mexico. J Am Mosq Control Assoc 14:369-372Villarreal C, Fuentes-Maldonado G, Rodriguez MH, Yuval B (1994) Low rates of multiple fertilization inparous Anopheles albimanus. J Am Mosq Control Assoc 10:67-69Yuval B, Fritz GN (1994) Multiple mating in female mosquitoes-evidence from a field population ofAnopheles freeborni (Diptera: Culicidae). Bull Entomol Res 84:137-140
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