Methods in Anopheles Research - MR4

More documents

Recommendations

Info

Chapter 2 : Anopheles Laboratory Biology and Culture2.4 Anopheles Culture2.4.1 Collecting Anopheles EggsPage 1 of 22.4 Anopheles Culture2.4.1 Collecting Anopheles EggsMR4 StaffIntroductionFor colony maintenance, we blood feed females only once in their lifetime, between 3 and 7 days postemergence(see Chapter 1, Rearing Schedule). After subsequent feedings, anophelines will lay eggsagain, which is helpful for maintaining a colony and having plenty of experimental material. Afterbloodfeeding, allow 2-3 days for embryo development prior to egging. Certain species may take moretime (An. dirus - 4 days), so you may have to modify your schedule according to the needs of yourparticular colony.Among the water types we have used, we have observed no effect on oviposition. While there are almostcertainly measurable differences that would be important in mass rearing facilities, an excess of eggsbeyond what is required for stock keeping is almost always obtained, and efficiency is not an issue.Most eggs are laid at night, and egg dishes are typically removed the day following their insertion. Thereare several ways to collect eggs. One method is to fill a cup with clean water to about 1 cm depth and linethe edges with filter paper such that half of the paper is submerged (Figure 2.4.1.1). The filter paperprevents the eggs from sticking to the dry plastic sides of the cup when it is sloshed.Other methods include allowing mosquitoes to lay eggs on wet filter paper supported by wet cotton orsponges which are then floated in water the next day, filter paper funnels partially submerged in water,and dark bowls lined with filter paper (Figure 2.4.1.2-Figure 2.4.1.4).Regardless of the method used, after collecting eggs, remove all dead adults to help prevent carryinginfections into the new larva pans. Adults can be removed individually with forceps or filtered out bywashing the egg/adult mix through a screen and collecting the eggs beneath.Figure 2.4.1.1. Egg collection cupcontaining standing water to a depth that isapproximately half way up the filter paperliner.Figure 2.4.1.2. Filter paper on a damp sponge in aplastic dish. The sides are lined with either white orblack paper, and it is apparent that An. gambiae andarabiensis prefer resting on the latter for oviposition.
Chapter 2 : Anopheles Laboratory Biology and Culture2.4 Anopheles Culture2.4.1 Collecting Anopheles EggsPage 2 of 2Figure 2.4.1.3. Filter paper funnel partiallysubmerged in water.Figure 2.4.1.4. Dark bowl lined with filter paper.
Page 1:
\Methods inAnopheles ResearchSecond
Page 8 and 9: Preface to Second EditionWhen Mark
Page 10 and 11: Chapter 1 : Insectary Operation1.1
Page 35 and 36: Chapter 2 : Anopheles Laboratory Bi
Page 57: Chapter 2 : Anopheles Laboratory Bi
Page 93 and 94: Chapter 2 : Anopheles Biology Labor
Page 107 and 108: Chapter 3 : Specific Anopheles Tech
Page 109 and 110:
Chapter 3 : Specific Anopheles Tech
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
5. Place eggs in a new Petri dish c
Page 117 and 118:
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
Page 125 and 126:
Page 127 and 128:
Page 129 and 130:
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Chapter 4 : Stock Authentication4.1
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
Figure 4.2.3.1 Lane 1 1kb ladder, l
Page 171 and 172:
Chapter 5 : Insecticide Resistance
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
INTRODUCTIONChapter 5 : Insecticide
Page 179 and 180:
Chapter 5 : Insecticide Resistance5
Page 181 and 182:
Page 183 and 184:
Page 185 and 186:
Page 187 and 188:
Page 189 and 190:
Page 191 and 192:
Page 193 and 194:
Page 195 and 196:
Page 197 and 198:
Page 199 and 200:
Page 201 and 202:
Page 203 and 204:
Page 205 and 206:
Page 207 and 208:
Page 209 and 210:
Page 211 and 212:
Page 213 and 214:
Page 215 and 216:
Page 217 and 218:
Page 219 and 220:
Page 221 and 222:
Page 223 and 224:
Page 225 and 226:
Chapter 6 : Dissection Techniques6.
Page 227 and 228:
Figure 6.2.1. Larval mosquito midgu
Page 229 and 230:
Page 231 and 232:
Page 233 and 234:
Page 235 and 236:
Page 237 and 238:
Page 239 and 240:
Page 241 and 242:
Page 243 and 244:
Page 245 and 246:
Page 247 and 248:
Page 249 and 250:
Page 251 and 252:
Page 253 and 254:
Chapter 7 : Taxonomy and Systematic
Page 255 and 256:
Page 257 and 258:
Page 259 and 260:
Page 261 and 262:
Page 263 and 264:
Page 265 and 266:
Collection NumberCountryNumber Le P
Page 267 and 268:
MOSQUITO BITING COLLECTION RECORDCo
Page 269 and 270:
Page 271 and 272:
Page 273 and 274:
Page 275 and 276:
Page 277 and 278:
Page 279 and 280:
Chapter 8 : Field Techniques8.1 Mol
Page 281 and 282:
Page 283 and 284:
Chapter 8 : Field Techniques8.2 Pla
Page 285 and 286:
Page 287 and 288:
Page 289 and 290:
Page 291 and 292:
Page 293 and 294:
Page 295 and 296:
Page 297 and 298:
Page 299 and 300:
Chapter 8 : Field Techniques8.4 Spe
Page 301 and 302:
Page 303 and 304:
Page 305 and 306:
Page 307 and 308:
Page 309 and 310:
Page 311 and 312:
Page 313 and 314:
Page 315 and 316:
Chapter 8 : Field Techniques8.5 Rea
Page 317 and 318:
Page 319 and 320:
Page 321 and 322:
Page 323 and 324:
Page 325 and 326:
20Chapter 8 : Field Techniques8.5 R
Page 327 and 328:
Page 329 and 330:
Page 331 and 332:
8070Mutant (S1014)Chapter 8 : Field
Page 333 and 334:
Page 335 and 336:
AChapter 8 : Field Techniques8.5 Re
Page 337 and 338:
Page 339 and 340:
Page 341 and 342:
Chapter 9 : Obtaining Live Material
Page 343:
Chapter 9 : Obtaining Live Material
show all

Methods in Anopheles Research - MR4

Create successful ePaper yourself

Delete template?

Save as template?