Full set of Notes with Fill-Ins - San Jose State University

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Chem 155 Unit 10 Page 192 of 316Single vs. double-beam instruments:Single-beam design: Measure blank signal (Po),digitize and record the number, replace blankwith sample cuvette, measure P, calculate A.Advantages of single beam designs:Simple design, relatively inexpensive.Disadvantages of single beam designs:P and Po are measured at different times so aparticular noise source is prominent:i.e. slow change in:o source intensityo detector sensitivitygivesPage 192 of 316driftabsorbance error.
Chem 155 Unit 10 Page 193 of 316Double-beam instruments continually monitoror alternate between P and Po using a second,blank cuvetteAdvantages of double beam designs:Accuracy:P and Po are measured:almost simultaneouslyso source and detector drift are:minimized!Hence absorbances are more:accurate.Disadvantages of double beam designs:Higher Cost: More complicated designOften:Often:Two detectorsMore opticsPage 193 of 316
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Full set of Notes with Fill-Ins - San Jose State University

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