Full set of Notes with Fill-Ins - San Jose State University

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Chem 155 Unit 17 Chromatography Page 306 of 316H A+ B + C⋅uuA2λ ⋅d pmultipath termB2γ ⋅D Mlongitudinal diffusionC = complex function of particlesize, coating, diffusion coefficient,favored in general by largediffusivity and small particle sizeresistance tomass transferPage 306 of 316
Chem 155 Unit 17 Chromatography Page 307 of 316General Elution Problem / Gradient ElutionA mixture of molecules with strongly differing k’ valuesis hard to separate because:If the solvent is weak enough to separate 1 and 2:a. Separation takes too longb.Peaks 5 and 6 broaden into baselineIf the solvent is strong enough to elute 5 and 6 in areasonable time:Peaks 1 and 2 and 3 and 4 co-elutePage 307 of 316
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