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Chem 155 Unit 17 Chromatography Page 310 of 316High Performance Liquid ChromatographySkoog Holler and Nieman Principles of Instrumental Analysis 5 th ed.Page 310 of 316
Chem 155 Unit 17 Chromatography Page 311 of 316Types of Liquid ChromatographyMost can be performed as HPLC in ‘highperformance’ instrumentation if necessary.Normal Phase:stationary phase: hydrophilic (SiO 2 particles)mobile phase: hydrophobic mobile phase(hexane, methanol).weak solvent: hexanestrong solvent: methanolretained: polar compoundseluted first: non-polar compounds1.1.70 Reverse Phase:stationary phase: hydrobic (SiO 2 particlescoated with hexadecane monolayer)mobile phase: hydrophilic mobile phase (water,methanol)weak solvent: waterstrong solvent: methanolretained: non-polar compoundseluted first: polar compoundsPage 311 of 316
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