Full set of Notes with Fill-Ins - San Jose State University

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Chem 155 Unit 1 Page 40 of 3164. What concentrations ‘bracket’ the 5.6 ppthtarget?For example:5.2, 5.4, 5.6, 5.8, 6.0 ppth--- or ---1, 2, 4, 6, 8 ppth--- or ---1, 2, 5, 10, 15 ppth--- or ---0.050, 0.50, 5.0, 50, 500 ppthDo you need to makestandards on an evenspacing?No – but calibrationpoints above andbelow the std. areneeded.Does the analytehave to fall right in themiddle of thecalibration standards?No, but itminimizeserror!5. What volumes of standards should you prepare?How much is needed by the instrument?What is the smallest volume that you canconveniently and precisely measure?How expensive is the analyte and solvent?How expensive is it to dispose of the waste?0.1 or 1 or 10 or 100 or 1000 mLPage 40 of 316
Chem 155 Unit 1 Page 41 of 3166. How do you prepare the calibration series fromthe primary stock solution?Primary Stock (1) is diluted to make Calibration Standard (2)C 1 V 1 = C 2 V 2First calibration standard:10 mL of 1 ppth sucrose from 100 ppth stocksolution.C 1 = 100 ppthC 2 = 1 ppthV 2 = 10.0 mLV 1 = ? = volume to pipet overV 1 = C 2 V 2 /C 1Page 41 of 316
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Full set of Notes with Fill-Ins - San Jose State University

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