Full set of Notes with Fill-Ins - San Jose State University

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Chem 155 Unit 14 248 of 316Strategies used to make IR spectrometry work -1. Poor sources and detectors:use interferometer instead of monochromator1. Optics absorb IR light:use mirrors instead of lenses2. Materials for transmissive optics:use high-atomic-mass materials with weakinter atomic bonds – typical materials are:1. CaF 2 2. ZnSe 3. KBr 4. NaCl3. Sample Handling:use solvents transparent in selected spectralregionsavoid solvents completelyuse reflection-absorption instead oftransmissionPage 248 of 316
Chem 155 Unit 14 249 of 316Solvents for IR spectroscopy:Handling of neat (pure – no solvent) liquids:Sandwich your sample between IR-transparentplates: e.g. NaCl:Q. If your sample is 10 microns thick, and theconcentration of molecules in the neat sample is10 M, and a typical band gives an absorbance of 0.1a. what is ε?A=εbC ε = A/bC = 0.1/0.001*10ε = 10b. why can’t you use a 1-cm pathlength NaCl cuvette?Page 249 of 316A=εbC = 10*1*10 = 100T=10 -100 = 0.00000000000000…….01
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AbsorbanceChem 155 Unit 11 196 of 3
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