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Chem 155 Unit 17 Chromatography Page 294 of 316Chromatography – Chapter 26General and descriptive aspects of chromatographicretention and separation: phenomenological k’,efficiency, selectivity.Quantitative description of zone migration in partitionchromatography: migration velocity, partitioncoefficient and theoretical k’.Theoretical description of efficiency: zone broadeningand the Van Deemter equation.Solving the general elution problem: Gradient vs.isocratic elutions.HPLC Instrumentation: pump, injector, column,detector, data collection.Chromatographic modes: HPLC (reverse and normalphase or ‘flash’), gel permeation (GPC), gel filtration,ion exchange.Electrophoresis.Page 294 of 316
Chem 155 Unit 17 Chromatography Page 295 of 316Chromatography: separation of chemicals in a mobilephase by differential flow rates through stationaryphase.Skoog Holler and Nieman Principles of Instrumental Analysis 5 th ed.Page 295 of 316
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