rologie i - European Congress of Virology

5 th European Congress of VirologyREF O149Factors for Adenovirus Infection and Disease in Pediatric HematopoieticStem Cell Transplant patientsLinda FEGHOUL 1 , Jean Hugues DALLE 2 , Sylvie CHEVRET 1 , MarieOUACHÉE 2 , André BARUCHEL 2 , Mony FAHD 2 , Valérie GUÉRIN ELKHOUROUJ 2 , Ghislaine STERKERS 2 , François SIMON 1 , JérômeLEGOFF 11 Université Paris Diderot, Hopital Saint Louis, Paris, FRANCE;2 Université Paris Diderot, Hopital Robert Debré, Paris, FRANCEAdenovirus (Adv) infections are critical in hematopoietic stem cells transplantation(HSCT). The aim was to determine risk factors for Adv infectionand disease in pediatric HSCT patients. Between September 2010 andDecember 2011, 73 pediatric HSCT patients were prospectively followedfor 12 months and weekly tested for adenovirus in blood and stool. Sheddingwas defined as a positive detection in stool, systemic infection aspositive detection in blood, and disease as systemic infection with Advsymptoms. Multivariable Cox models were used to define predictive factorsfor shedding in stool, systemic infection and disease with estimatedhazard ratio (HR) of event as a measure of association. Cumulative incidenceof shedding, infection and disease at 3 months post HSCT were 40%,23% and 17% respectively. Based on multivariable models, cord bloodtransplant was associated with shedding in stool (HR=4.29) and systemicinfection (HR=3.01). Acute GvHD was predictive of systemic infection(HR=1.46) and Adv disease (HR=2.55). For the patients with intestinalshedding, the occurrence of systemic infection was increased in case ofhigh viral load detected in stool (p=0.02). The sensitivity and specificity tohave a systemic infection were respectively 93.33% and 62.5% for loadsin stool above 5.36 log copies/ml. Conclusions: Cord blood transplant andacute GvHD appear the most appealing risk factors for AdV infection andshould be considered to initiate a molecular screening for Adv. Patientswith high AdV viral load in stool must be considered for early anti Advintervention.REF O150Level and kinetics of Plasma Torque Teno Virus DNA after LungTransplantation as a Marker to Guide Immunosuppressive TherapyIrene GOERZER 1 , Mats HALOSCHAN 1 , Peter JAKSCH 2 , IsabellaBEJVL 1 , Robert STRASSL 3 , Walter KLEPETKO 2 , ElisabethPUCHHAMMER STOECKL 11 Department of Virology, Medical University of Vienna, Vienna, AUSTRIA;2 Division of Thoracic Surgery, Medical University of Vienna, Vienna,AUSTRIA; 3 Clinical Institute of Virology, Medical University of Vienna,Vienna, AUSTRIATorque teno virus (TTV), widely spread among humans, causes persistentviremia in healthy persons probably without clinical disease. Transplantrecipients receive immunosuppressive therapy. Its optimization is criticalto avoid rejection or infection, but it is still a challenge as appropriate markersreflecting the state of immunosuppression are lacking. In this study,we investigated whether level and kinetics of TTV DNAemia could reflectthe patient’s immunosuppression after lung transplantation. Plasma TTVDNA kinetics was analyzed by quantitative PCR in 31 lung transplant recipients(LTRs) over 2 years posttransplantation. Its relation to tacrolimus(Tac) versus cyclosporine (CsA), to infection events and to developmentof bronchiolitis obliterans syndrome (BOS) was assessed. In all LTRs,TTV DNA significantly increased up to day 90 posttransplantation andsustained high TTV levels thereafter. The extent of TTV increase wasassociated with mean drug concentrations (p=0.012) and mean TTV levelsthereafter were lower in CsA treated than in Tac treated patients (p