rologie i - European Congress of Virology

5 th European Congress of VirologyBlood transfusion was noted in 46.2% of cases. HCV prevalence was20.5%. Two HCV seroconversion cases (1.4%) were diagnosed. Conclusion:beside longer hemodialysis duration, blood transfusion remains amajor risk factor of HCV infection in hemodialysis patients despite thesystematic anti HCV screening in blood donors. The nosocomial transmissionis certain in hemodialysis departments. More respect of Hygienerules and adapted strategy are needed to prevent HCV transmission.REF 374Preliminary results on Human T cell lymphotropic virus HTLV ½identification among the blood donorsSpinu IGOR, Guriev VLADIMIR, Spinu CONSTANTINNational Center for Public Health, Chisinau, REPUBLIC OF MOLDOVACurrently, habilitate requirements submitted by the structures of the ECto the service of blood include testing of mandatory donated blood, bloodcomponents, including stem cells to the presence of markers of viral hemotransmisibileinfections: infection with viral hepatitis B, C, HIV infection,cytomegalovirus and infection with HTLV 1 and HTLV 2 (Human Tcell lymphotropic virus HTLV ½). Laboratory investigations algorithmin national blood service achieved by the insurance for the biosafety riskexclusion from the hemotransmisibile transmitting of viral etiology infectionsincludes testing of the donors for human imunodificiency virus andviral hepatitis B and C. In this context a special scientific practically interestpresents data to presence of this Human T cell lymphotropic virusamong on blood donors. The results show that during investigation ofblood samples collected from donors in number of 258 people aged 19 to59 years, five persons were HTLV positive. Preliminary obtained resultsallowing us to following: to organize together with donor service additionalresearches on markers HTLV for donors of blood; studying the possibilityof supplementing the donors blood investigation on infections by viruseshepatitis B, C and HIV, with additional HTLV virus, in the National Centersfor blood transfusion, reducing the risk of post transfusional disease,caused by the named virus; extending these studies and the other contingentsof population in particular with increased risks of infection withhepatitis viruses B, C and HIV, taking into account the fact that associateways of transmission.REF 375Distribution of Torque Teno Viruses (TTV) in healthy Bulgarian populationZlatko KALVATCHEV 1 , Iliya TSEKOV 1 , Kalina SHISHKOVA 2 ,Rumen POPOV 3 , Stoyan SHISHKOV 21 Military Medical Academy, Center for Virology Diagnosis, Sofia, BUL-GARIA; 2 University of Sofia “St. Kl. Ohridski ”, Faculty of Biology,Laboratory of Virology, Sofia, BULGARIA; 3 Military Medical Academy,Center of Transfusional Haematology, Sofia, BULGARIABackground: Torque teno viruses (TTV) are novel circular, single strandedDNA viruses first identified in patients with post transfusion hepatitisof non A G type. The prevalence and genetic heterogeneity of TTV isshown in patients with various diseases, but TTV are not linked to a particularcondition. TTV are widespread and relatively little is known abouttheir pathogenic potential, especially among healthy humans. Also datais not present for the prevalence of TTV in the Bulgarian population.The present study analyzed TTV loads in two groups of clinically healthypeople that donated blood or performed prophylactic examinations.Methods: Groups of 40 healthy people and 40 blood donors, which werenegative for common pathogens such as HIV, HBV, HCV, CMV, EBVwere included. DNA extraction from blood samples was performed withthe QIAamp DNA Blood Mini Kit (QIAGEN GmbH, Germany) and TTVsequences were amplified in nested PCR with primers for the 5 ′ UTR ofthe viruses. The amplification product was visualized on an ethidium bromidestained 2% agarose gel. Results and Discussion: This is the firstreport to demonstrate TTV among Bulgarians with a prevalence of 46/80(57.5%). These results indicate the widespread of TTV among healthyindividuals and correspond to the data published by other authors, whichreport the viruses in up to 90% of the human population. The adaptedPCR system is effective and can be used for large scale screening studiesconcerning the prevalence of TTV in Bulgaria, as well as to clarify therole and significance of these viruses in human pathology.REF 376Human parechovirus 4 Caused Neonatal Cases with Suspected Sepsisin FinlandPekka KOLEHMAINEN 1,2 , Anne JÄÄSKELÄINEN 1,3 , HannimariKALLIO KOKKO 1,3 , Tea NIEMINEN 4 , Marjaleena KOSKINIEMI 1 ,Sisko TAURIAINEN 2 , Maija LAPPALAINEN 31 Haartman Institute/University of Helsinki, Helsinki, FINLAND;2 University of Turku, Turku, FINLAND; 3 HUSLAB/Helsinki UniversityCentral Hospital, Helsinki, FINLAND; 4 HUS/Helsinki University CentralHospital, Helsinki, FINLANDHuman parechoviruses (HPeVs), close relatives of enteroviruses, havebeen associated with neonatal sepsis like syndrome, central nervous systeminfections, respiratory symptoms and gastrointestinal infection. Whilemost HPeV types are primarily associated with infections of a mildoutcome, HPeV3 has been observed to be responsible for more severeinfections. HPeV4, originally extracted from a child with fever, has notbeen linked to more severe infections. In October, 2012 two neonates aged1 and 2 months were hospitalized with suspected sepsis in Finland. Bothof the patients had high fever and one of them had marbleizing skin, whichdeveloped in three days into an enterovirus like macular rash. No causativeagent was detected with routine bacterial and viral diagnostics from blood,urine, spinal fluid and stool samples. Samples were then directed to HPeVreal time PCR. Both patients were positive for HPeV in stool and one alsoin serum. The causative agent was typed to be HPeV4 in both cases. Inthis study we show that HPeV4 may cause more serious infections withdermatological manifestations and sepsis like symptoms. Additionally, weconfirm that more severe HPeV infections occur also in Finland. Theseare first reported HPeV4 cases in Finland. Patients with neonatal sepsis orsuspected sepsis require often intensive care and thus clarifying its causativeagent is essential. These cases underline the importance of HPeVinfection diagnosis as equally vital as that of enteroviral infection.REF 377Extensive genetic variation of seasonal A/H3N2 influenza viruses inGreece during the winter period 2011 2012Athanasios KOSSYVAKIS 1 , Angeliki MELIDOU 2 , Maria EXINDARI 2 ,Georgia GIOULA 2 , Antonios KALLIAROPOULOS 1 , VasilikiPOGKA 1 , Afroditi MOUTOUSI 1 , Mary EMMANOUIL 1 , AndreasMENTIS 1 , Sotirios TSIODRAS 3 , Petros KARAKITSOS 4 , NikolaosMALISIOVAS 21 National Influenza Reference Laboratory for S. Greece, Hellenic PasteurInstitute, Athens, GREECE; 2 Laboratory Department, MedicalSchool, Aristotle University of Thessaloniki, Thessaloniki, GREECE;3 Hellenic Centre for Disease Control and Prevention, Athens, GREECE;4 Department of Cytopathology, University General Hospital “Attikon”,School of Medicine, National and Kapodistrian University of, Athens,GREECEObjectives: this study aimed to identify emergence of genetic and antigenicvariation of A (H3N2) strains circulated in Greece during 2011-2012winter period. Methods: respiratory specimens from influenza like ill-S224 Virologie, Vol 17, supplément 2, septembre 2013