rologie i - European Congress of Virology

5 th European Congress of Virologyhigh prevalence of Asian ethnic background (41% and 83%) and commoncross border contacts with China (45%). Only 24 out of over 4000 nonpolio enteroviruses isolated in Russia and neighbouring countries in 20012012 were identified as EV71, genotype C. Only two isolates belongedto GtC1. Subgenotype C2 dominated from 2001 until 2012. Subtype C4emerged in Russia in 2009 and became common in 2011 2012. Phylogeneticevidence suggests that 18 non identical EV71 isolates resulted from14 independent introductions, presumably from Europe (GtC1 and GtC2)and China (GtC4). Despite high seroprevalence and detectable virus circulation,there has been no outbreaks of HFMD and neuroinfection causedby EV71 in Russia. It is therefore likely that host factors play an importantrole in EV71 epidemiology and pathogenesis.REF 367Seroprevalence of hepatitis B in the Mohamed V Military Hospital inRabatNada BENJELLOUN, Mohamed Rida TAGAJDID, BouchraBELFQUIH, Hicham ELANNAZ, Saâd MRANILaboratory of Virology, HMIM V, Faculty of Medicine and Pharmacy,University Mohamed V Souissi, Rabat, MOROCCOIntroduction: this study was conducted to determine the seroprevalenceof hepatitis B virus in the Mohammed V military hospital (HMIMV) andto study clinical and biological characteristics of the patients included inthe study.Patients and Methods: this is a retrospective study conducted in thelaboratory of Virology over a period of 13 months between 01/01/2012and 18/02/2013. Epidemiological (age, sex, service) and biological data(HBsAg, anti HBc Ab, Ac antiHBs, Ac antiHBe, HBeAg, viral load, lookingfor a co infection) of patients with HBsAg positive were collected onthe package “Laboserver ”. Serological tests were performed by ELISA.Determination of the HBV viral load was performed by real time PCRCobasTaqman instruments. Results: 9723 samples were tested for HBsAgduring the study period. The HBsAg was positive in 261 cases (2.68%).Among patients with HBsAg positive, 162/169 had positive anti HBc Ab,90/98 had positive anti HBe, 2/105 had anti HBs positive and 41/134 had anundetectable viral load. Co infection HBV HIV was detected in 3 patientsof 162 tested, and no co infection HBV HCV was encountered. Discussion:in Morocco, there is little information available on the prevalenceof hepatitis B, but it was listed in the area of low endemicity, with a prevalenceof HBsAg between 2 and 7%, this data is approved by our study(2.68%). This decline in the incidence and prevalence of HBV infectionis particularly due to the vaccination program. Median seroprevalence ofanti HBc Ab and anti HBe Ab followed a trend similar to that of HBsAg,as shown in our study. In the literature, the average age of infected patientswas 41 ± 12.4 years, with a male predominance, these data are consistentwith those obtained HMIMV. The study of viral load can’t be used on firstline because of the high cost and technical complex.REF 368Monitoring and comparison of the burden of different respiratoryviruses in primary healthcare units and in hospitals at NationalInfluenza Centre in SloveniaNatasa BERGINC, Katarina PROSENC TRILARLaboratory for Virology, National Institute of Public Health, Ljubljana,SLOVENIAFrom week 38/2011 to 11/2013 3147 swabs of patients with influenzalike illness and acute respiratory tract infection, their personal and clinicaldata were collected from 45 primary healthcare(PHC) doctors and 2hospitals(H). 6 age groups(AG) were formed: 0 3, 4 7, 8 14, 15 19, 20 64,>65 years of age(YA). Influenza(Flu), respiratory syncytial virus(RSV),adenovirus(AV), enterovirus(EV), rhinovirus(RV), parainfluenza, metapneumovirus,bocavirus were detected in multiplex RT RT PCR. Resultsare analysed to monitor the burden of this viruses in PHC and in H. In PHCin AG of 0 3YA Flu, RSV, AV are most often detected (37%, 25%, 20%respectively) while in other AGs Flu is most often detected (70% 83%). InH in young chilrden of 0 3YA AV, RV, RSV, EV, Flu are most often detected(27%, 26%, 20%, 10%, 9% respectively). In children and teenagersAV, Flu, RV are most often detected (18%, 16%, 7%, AG 4 7YA; 25%,25%, 25%, AG 8 14YA; 36%, 19%, 16%, AG 15 19YA respectively). Inadults and elderly Flu is mostly detected (73%, AG 20 64YA; 70%, AG>65YA respectively), followed by RSV, AV, RV (6%, 12%, 6%, AG 2064YA; 14%, 5%, 5%, AG >65YA respectively). Young children are moreoften diagnosed with bronciolitis than pneumonia (30%, 7% respectively)but adults and elderly with pneumonia than bronciolitis (22%, 6%, AG 2064YA; 30%, 15%, AG >65YA respectively). Children and teenagers areequally diagnosed with both. In PHC Flu represents the highest burden inall AGs. In H AV, RV, RSV, Flu represent the highest burden in childrenand Flu represents the highest burden in adults and elderly.REF 369Characteristisc of influenza season 2012/2013 and differences in comparisonto previous Influenza Seasons in SloveniaNatasa BERGINC, Katarina PROSENC TRILARLaboratory for Virology, National Institute of Public Health, Ljubljana,SLOVENIAAt National Influenza Centre specimens from patients with influenza likeillness and acute respiratory tract infections with personal, clinical andepidemiological data are collected from a sentinel of 45 primary healthcaredoctors and 2 hospitals. Detection and subtyping of influenza (Flu)is performed with RT RT PCR: FluA subtypes AH1, AH1pdm, AH3,AH5 and FluB lineages Victoria (Vic), Yamagata (Yam). Usually in theinfluenza season (IS) one subtype predominates (IS 2004/05 FluB, IS2006/07 AH3, IS 2007/08 AH1, IS 2009/10 AH1pdm). It is commonthat in the end of an IS another subtype overcomes the predominant one(most often FluB overcomes FluA: IS 2008/09 FluB overcame AH3). InIS 2010/11 AH1pdm and FluB had equal shares trough the whole IS.During the pandemic in 2009 an unusual IS was observed with 2 peaks: insummer and in autumn with predomination of AH1pdm. An average peakof an IS lasts 6 weeks (W). IS 2012/13 differs from others in 2 aspects:constant simultaneous circulation of 4 different Flu and a duration. Atthe start of IS there were 70% FluA, 30% FluB. That changed graduallyduring the IS to 30% FluA, 70% FluB. Amongst FluA H1pdm and H3were present in relatively stable ratio (70%, 30% respectively). AmongstFluB Yam and Vic were constantly present with a changing ratio: at thebeginning Yam predominated over Vic, in the middle they were presentin equal ratios and towards the end Yam overcame Vic again. The firstFlu (AH1pdm) was detected in W49/2012, the peak lasted from W4/2013to at least W12/2013 (abstract submission), so it was at least 8 weekslong.REF 370Hepatitis E virus epidemiology: is serology sufficient?Sylvia BRUISTEN 1 , Kelly VAN DIJK 1 , Martijn VAN ROOIJEN 1 ,David KWA 2 , Boris HOGEMA 3 , Gini VAN RIJCKEVORSEL 11 Public health service of Amsterdam, Amsterdam, THE NETHERLANDS;2 OLVG General Hospital, Amsterdam, THE NETHERLANDS; 3 SanquinBlood bank, Amsterdam, THE NETHERLANDSNon travel related hepatitis E virus (HEV) infections are reported toincrease in many industrialized countries. A high prevalence of HEVin the general population may be indicative for the threat for HEVS222 Virologie, Vol 17, supplément 2, septembre 2013