rologie i - European Congress of Virology

5 th European Congress of VirologyIn this study, 150 blood sera samples of unvaccinated domestic horseswere tested for equine herpesvirus (EHV 1, EHV 4) specific antibodies bycommercially available indirect Enzyme Linked Immunosorbent Assay(ELISA, Svanovir, EHV 1/EHV 4 Ab kit, Svanova Biotech AB, Sweden).EHV 1 and EHV 4 specific antibodies were detected in 52 (34.66%) and101 (67.33%) of the 150 tested sera, respectively. The results indicatedthat EHV 1 and EHV 4 infections appear to be widespread in domestichorses in South East Anatolia.Key words: EHV 1, EHV 4, ELISA, horseREF 312Detection of antibodies against Blue Tongue Virus in Yaks (BOSGRUNNIENS) in Issyk KulOguzhan AVCI 1 , Orhan YAPICI 2 , Oya BULUT 1 , Mehmet KALE 3 ,Sibel YAVRU 1 , Atilla SIMSEK 11 University of Selcuk, Faculty of Veterinary Medicine, Department of Virology,KONYA, TURKEY; 2 University of Kyrgyzstan Turkey Manas, Facultyof Veterinary Medicine, Department of Virology, BISHKEK, KYRGYZS-TAN; 3 University of Mehmet Akif Ersoy, Faculty of Veterinary Medicine,Department of Virology, BURDUR, TURKEYBluetongue virus (BTV) is the type species of the genus Orbivirus withinthe family Reoviridae. There are 26 BTV serotypes that are determinedby the specificity of interactions between the virus outer capsid with neutralizingantibodies generated during infection of the ruminant host. Theaim of this study was to describe the seroprevalence rate of bluetonguevirus (BTV) in unvaccinated yaks in Issyk Kul. A total of 168 serumsamples were collected from yaks that were randomly selected betweenJuly August 2011. Antibodies to BTV in sera were detected using a commerciallyavailable competitive enzyme linked immunosorbent assay (cELISA, VMRD, USA). The test was performed as per the manufacturer’sinstructions. The results showed that 4 (2.38%) of the samples werepositive for BTV antibodies. This study indicates that antibodies againstBTV are uncommon among the yaks in Issyk Kul. In spite of the significanteconomic and animal health impacts of BTV infection in sheep, todate there has been no assessment of the Culicoides spp. involved. Despitethe low results large scale research is necessary for the prevention ofBTV infection. In conclusion, the seroprevalence of BTV in yaks has beeninvestigated for the first time in Issyk Kul.Key words: BTV, yak, ELISAREF 313New subtypes of small ruminant lentiviruses (SRLVs) detected inSlovenian goat and sheep flocksDarja BARLIC MAGANJA 3 , Urska KUHAR 1 , Joze GROM 21 University of Ljubljana, Veterinary Faculty, Ljubljana, SLOVENIA;2 University of Ljubljana, Veterinary Faculty, Ljubljana, SLOVENIA;3 University of Primorska, Facutly for Health Sciences, Izola, SLOVENIASmall ruminant lentiviruses (SRLVs) of the Retroviridae family infectgoats and sheep worldwide. Phylogenetic analysis divides them into fiveprincipal genotypes (A E). The Slovenian SRLV strains characterized byphylogenetic analysis of two genomic regions (1.8 kb gag pol fragmentand 1.2 kb pol fragment) were clustered into A and B genotypes. Theyare highly heterogeneous, with ovine strains belonging to genotype A andcaprine strains to genotypes A and B. A cluster composed of four sheepvirus sequences was clearly divergent from all other subtypes in group Aand could not be assigned to any of them and two goat virus sequenceswere found to belong to genotype A and could not be assigned to existingsubtypes. The results of phylogenetic analysis performed in our studyrevealed two new subtypes within genotype A, subtype A14 and subtypeA15.REF 314Characterization of pandemic influenza A(H1N1) 2009 viruses isolatedfrom Pig and Cat in France since 2009Emilie BONIN 1,2 , Séverine HERVÉ 1,2 , Aure SAULNIER 1,2 , StéphaneQUÉGUINER 1,2 , Nicolas BARBIER 1,2 , Stéphane GORIN 1,2 , GaëlleSIMON 1,21 Anses, Laboratoire de Ploufragan Plouzané, Unité Virologie ImmunologiePorcines, Ploufragan, FRANCE; 2 Université Européenne de Bretagne,FRANCEIn April 2009, a novel influenza A(H1N1) virus (H1N1pdm) was detectedin people in Mexico and the United States. The virus spread rapidly andcaused a flu pandemic in humans within a few months. The new viruscontains a combination of genomic segments from swine origin that hadnever been identified previously. Soon after the virus started to spread globallyin humans, its introduction into pig holdings was noticed in manycountries, including European member states. Transmission of H1N1pdmto French pigs was evidenced to have occurred in Brittany as early asJanuary 2010. However, the virus does not seem to have been maintainedwithin the pig population in this region of high pig density where almost50% of the herds are infected by European enzootic swine influenza viruses(SIVs) for many years. By contrast, H1N1pdm was regularly isolated frompig herds located in central part of France since October 2010, demonstratingthat the virus spread in the French pig population, especially inareas with most naive animals. Not only restricted to humans and pigs,H1N1pdm virus was also detected in turkeys, cheetahs, minks, seals, ferrets,dogs and cats. In November 2009, it was isolated from a domesticcat in France, after several members of the family contracted the infection.Whole genomes of H1N1pdm viruses isolated from pigs and cat inFrance were sequenced. Phylogenetic and polymorphism analyses showedthat all genomic segments were very closely related to respective counterpartsfrom other contemporary pandemic strains isolated in Europe andto reference strain A/California/04/2009. To date, no reassortant virusesbetween H1N1pdm and enzootic SIVs were detected in France unlike inother European countries, but may appear. The spread of H1N1pdm intopig population is an additional risk for the emergence of new influenzaviruses with zoonotic potential.REF 315Serological and virological investigation of Canine Adenovirus Infectionin dogsOya BULUT 1 , Orhan YAPICI 2 , Oguzhan AVCI 1 , Atilla SIMSEK 1 ,Kamil ATLI 1 , Irmak DIK 1 , Sibel YAVRU 1 , Sibel HASIRCIOGLU 3 ,Mehmet KALE 3 , Nuri MAMAK 41 University of Selcuk, Faculty of Veterinary Medicine, Department of Virology,Konya, TURKEY; 2 University of Kyrgyzstan Turkey Manas, Facultyof Veterinary Medicine, Department of Virology, Bishkek, KYRGYZSTAN;3 University of Mehmet Akif Ersoy, Faculty of Veterinary Medicine, Departmentof Virology, Burdur, TURKEY; 4 University of Mehmet Akif Ersoy,Faculty of Veterinary Medicine, Department of Internal Medicine, Burdur,TURKEYAdenoviruses are linear, double stranded DNA viruses which infect a widevariety of mammals and birds. Two have been identified in the dog: CanineAdenovirus type 1 (CAV 1) which infects most of the major organs causing,amongst other diseases, canine infectious hepatitis, and CAV 2 whichcauses canine infectious laryngotracheitis and enteric diseases. The aimof this study is to define the serological and virological status of CAVinfection in dogs. In this study, blood serum samples were taken from111 dogs that show clinical signs from the clinics of Veterinary MedicineFaculty, Selcuk University. Also 77 dogs were sampled from Ispartaand Burdur dog shelters by random sampling, regardless of the clinicalfindings. Clinical findings included a systemic disease, characterized byfever, vomiting, mucopurulent oculonasal discharge and conjunctivitis,Virologie, Vol 17, supplément 2, septembre 2013S207