rologie i - European Congress of Virology

5 th European Congress of Virologyinaudible fetal heartbeat. The ultrasound revealed a single fetus with noactive movements, little or absente amniotic fluid and placenta with reduceddimensions. The fetus was 32/33 weeks presenting placental abruptionwith apparently early detachment. During the caesarean there was largeextravasation of blood in cavity, deterioration of clinical picture, hypothermia,anuria, hemodynamic instability, mydriasis, absent reflexes anddeath. Tissues of spleen, placenta and umbilical cord were paraffin embeddedto investigate dengue virus. The RNA was extracted from sections of3 m and RNA extraction was performed using the kit Purelink FFPERNA Isolation Invitrogen. Real time PCR and RT PCR was performedand DENV 2 detected by both methods in the umbilical cord.Considering that dengue infection during pregnancy may represent a risk tomother and concept, measures to strengthen epidemiological surveillanceand virological diagnosis should be implemented to this specific groupespecially in dengue endemic areas.REF 488Prognostic significance of intra-uterine detection of infectious agentsOlga OSTROVSKAYA, Nataliya IVAKHNISHINA, ElenaNAGOVITSYNA, Marina VLASOVAResearch Institute of Mother and Child Health Care, Khabarovsk, RUSSIADNA of infectious agents was analyzed by a PCR method in placentalsamples of 66 pregnant women who had placental insufficiency and ahigh risk of intrauterine infection development. Infectious agents werefound in 72.7% cases of placental analysis. Ur.urealyticum (51.5%) andM. hominis (24.2%) were most frequent foiund, followed by type 4, 5 and6 herpesviruses. Enteroviruses, C. trachomatis, S. pneumoniae were foundin single cases. No other pathogens were detected.Infected placentas were significantly more frequently associated withabnormal peri-natal features such as untimely discharge of amnioticfluid, decompensation of chronic placental insufficiency, delivery ofpremature newborns with very to extremely low body weight, withdevelopmental delay and clinical signs of neonatal infection (pneumonia,hepatosplenomegaly,necrotizing enterocolitis, retinopathy, etc.)Detection of intrauterine infectious agents in placenta may be used forprediction of the newborn’s condition and early administration of adequatetherapy.REF 489Case report of a congenital Echovirus 11 infection acquired early inpregnancyChristelle VAULOUP FELLOUS 5 , Mikael TASSIN 1 , JelenaMARTINOVIC 2 , Audrey MIRAND 3 , Hélène PEIGUE LAFEUILLE 3 ,Olivier PICONE 4 , Alexandra BENACHI 11 AP HP, Department of Gynecology and Obstetrics, Antoine BéclèreHospital, Clamart, FRANCE; 2 AP HP, Unit of Fetal Pathology, AntoineBéclère Hospital, Clamart, FRANCE; 3 Laboratory of Virology, EnterovirusNational Reference Centre, Clermont Ferrand Hospital, ClermontFerrand, FRANCE; 4 Department of Gynecology and Obstetrics, FochHospital, Suresnes, FRANCE; 5 AP HP, Laboratory of Virology, RubellaMaterno foetal infection National Reference Center, Paul Brousse Hospital,Villejuif, FRANCEEnterovirus (EV) maternal infection during pregnancy and its relation tofetal pathology are not well described. When reported, the main manifestationsof EV congenital infections are intra uterine fetal death ormyocarditis. No information on intrauterine Echovirus 11 infection or theeffect of transplacental Echovirus 11 infection on development of the fetushas been described in literature up to date (excluding late pregnancy infections).We report here a case of an extreme form of pulmonary hypoplasiain a neonate, characterized by total failure of development of terminal respiratoryunits. This bichorial biamniotic twin pregnancy, was marked byspontaneous demise of the co twin at 14 weeks of gestation (WG), as wellas by positive PCR for EV (Echovirus 11 serotype) in the amniotic fluid,performed for moderate pericardial effusion at 22 WG. No sign of cardiacdisease were further observed, but at 32 WG an ultrasound examinationrevealed bilateral abnormal lung development, and pulmonary hypoplasiawas confirmed by MRI. After spontaneous delivery at 38 WG, and despitepediatric reanimation, the child died one hour after birth. The coexistenceof a congenital EV infection and a severe primary pulmonary hypoplasia,in this case, challenges our understanding of the pathogenesis of thissevere pulmonary growth arrest. The study of more cases of congenitalEV infections acquired early in pregnancy is needed to better understandthis puzzling lethal condition.REF 490Infants born to HBsAg(+) Mothers Are they fully protected afteractive passive vaccination?Radka KOMITOVA 1 , Maria NESHEROVA 2 , Maria ATANASOVA 31 Infectious Diseases Dept, University Hospital, Plovdiv, BULGARIA;2 Neonatology Unit, Selena Hospital, Plovdiv, BULGARIA; 3 Microbiologyand Immunology Laboratory, Medical University, Plovdiv, BULGARIAIntroduction: Infants born to HBsAg(+) mothers are at ongoing risk ofhepatitis B virus (HBV) infection. Despite the timely active-passive vaccination,newborns may still become HBV infected because of nonresponseto vaccination.Aims: to confirm that the child born to an HBsAg(+) mother is fullyprotected by active-passive immunization.Case presentation: A girl was born on 07.04.2012 by cesarean sectionto a mother with chronic HBV infection [(HBsAg(+), antiBHctotal(+),HBeAg(-), antiHBe(+)]. The infant received the 1-st shot hepatitis B vaccineas well as hepatitis B immunoglobulin (HBIG) within 6 hours afterbirth. The test conducted on 07.01.2013 (3 months after administration ofthe third vaccine shot) revealed an HBsAg(-) result and antiHBs level 1225IU/ml, i. e. she is thoroughly protected and does not need further management.A second study is underway with a girl born to an HBsAg(+)mother on 30.11.2012. She also received the 1-st shot hepatitis B vaccineas well as HIBG within 6 hours after birth. At the age of 9 months shewill be tested for HBsAg and antiHBs level to make sure protection ispresent. In case of HbsAg(-) and antiHBs level