rologie i - European Congress of Virology

5 th European Congress of Virologyof this virus and the vaccine strains used (which belongs to genotype A) aswell as the vaccine effectiveness (during outbreaks) of 61.6% to 70% forJeryl Lynn strain and 0% to 12.4% for Rubini may explain the high numberof cases in this outbreak involving vaccinated teenagers. Additional studiesare needed to clarify this vaccine failure.Acknowledgements: The authors gratefully acknowledge to the patientsand to the public health staff which contributed, respectively, with samplesand information about each mumps cases.REF 393Results of the retrospective analysis of HPV genotypes at YeditepeUniversity Hospital from 2008 to 2012Gulden CELIK(YILMAZ) 1 , Iskender KARALTI 2 , Yesim GUROL 1 ,Cagatay ACUNER 1 , Burcu OKSUZ 1 , Pinar OZCAN 3 , YaseminOZTURK 1 , Baki EKCI 4 , Sahap AKSACLI 1 , N. Cem FICICIOGLU 3 ,Ozcan GOKCE 41 Yeditepe University Faculty of Medicine, Medical Microbiology, Istanbul,TURKEY; 2 Yeditepe University Faculty of Medicine, Faculty of HealthSciences, Istanbul, TURKEY; 3 Yeditepe University Faculty of Medicine,Obstetrics and Gynecology, Istanbul, TURKEY; 4 Yeditepe UniversityFaculty of Medicine, General Surgery, Istanbul, TURKEYObjective: to evaluate the results of retrospective analysis of HPV genotypesdetermined by multiplex PCR and reverse hibridization techniquesin the Microbiology Laboratory Yeditepe University Hospital. Methods:149 clinical samples (125 of them female and 23 of them male) werecollected from patients of different ages and sex who applied to differentclinics of Yeditepe University Hospital tested for HPV from May 2008to April 2012. Nucleic acid isolation of the genital samples were madeby recommended isolation kit (Roche Magna Pure Compact Nucleic AcidKit) by the company. For HPV DNA detection and typing, multiplex PCRand reverse hybridization techniques were used by Hybribio HPV Genoaraykit. Results: HPV DNA was detected in 47.2% of the female and 58%of the male patients. The HPV PCR positivity for the women and men, forhigh risk group was 67.8%, 13%; for low risk group 27.1%, 86.7% andundefined risk group 5.1% and 0.3% respectively. Of the 34 high risk HPVpositive samples which were from departmant of Obstetrics and Gynecoloy;type 16 was detected in 10 patients; type 31 in 7 patients; each oneof type 58 and type 66 in 3 patients; each one of type 18, type 52, type 33and type 56 in 2 patients; and each one of type 59, type 39 and type 45 inone patient. Of the 12 low risk HPV positive samples; type 6 was detectedonly in 5, type 11 only in 3, type 44 only in 2, type 43 and type CP8304only 1 patients. Type 53 was detected in 8 patients. More than one HPVgenotypes (mixed infection) were detected in9 clinical samples.Conclusion: although the study group was small, variability of HPVgenotypes was found to be worth presenting.REF 394Global WHO Measles-Rubella Laboratory Network – molecular surveillanceto support the disease elimination initiativesMick N. MULDERS, David FEATHERSTONE, Paul ROTA, JosephICENOGLE, Kevin BROWN, Richard MYERS, Peter STREBEL1 World Health Organization, Geneva, SWITZERLAND; 2 Independentlaboratory consultant, Hastings, NEW ZEALAND; 3 Centers for DiseasesControl and Prevention, Atlanta, USA; 4 Centers for Diseases Control andPrevention, Atlanta, USA; 5 Public Health England, London, UK; 6 PublicHealth England, London, UK; 7 World Health Organization, Geneva,SWITZERLANDIn the Global Vaccine Action Plan, measles and rubella are targeted forelimination in at least five of the six WHO Regions by 2020. Rapidand accurate diagnosis of measles and rubella is essential for monitoringprogress and detecting outbreaks. WHO coordinates a global MRLaboratory Network (LabNet) that provides valuable global informationabout the circulation of measles and rubella virus. It consists of 690 national,sub-national and regional laboratories, serving almost all memberstates. The laboratories follow standardized testing protocols and reportingmechanisms that are constantly reviewed and improved as technologicalinnovations occur. The LabNet relies on a strong quality assurance programthat monitors the performance of all laboratories through annualproficiency testing and continuous assessment. It also provides vital informationfor the immunization programs as it documents the successes ofvaccination efforts to interrupt measles and rubella chains of transmission,monitor virus transmission patterns and help document successfulelimination strategies. Examples of which will be given including theWHO genotyping databases MeaNS and RubeNS. Both databases havebeen developed by Public Health England together with the WHO GlobalMR LabNet to develop a web-accessible and quality-controlled nucleotidedatabase as tool to track measles and rubella sequence diversity andmonitor elimination of virus strains. At the time of submission, MeaNScontained well over 12000 viral sequences, and RubeNS is still in its pilotphase with close to 1000 sequences. The databases have been developedspecifically for the MR LabNet with only secure access.http://www.who-measles.orgREF 395Molecular Characterization of Barley Yellow Dwarf Virus isolatesfrom Different Regions of TunisiaMaryem BOUALLEGUE 1 , Maha MEZGHANI-KHEMAKHEM 1 ,Hanem MAKNI 1,2 , Mohamed MAKNI 11 RU “Genomics of devastating insects of agricultural interest crops”GIRC, Faculty of Sciences of Tunis, Tunis El-Manar University, Tunis,TUNISIA; 2 ISAJC Bir El Bey, Tunis University, Tunis, TUNISIAThe Barley Yellow Dwarf Virus assigned to the Luteoviridae, is transmittedin a persistent manner and circulated by several aphid species mainlyRhopalosiphum padi, Sitobion avenae and Schizaphis graminum. Thisvirus infects many hosts of Poaceae and causes reducing yield in grainwhich leading to serious agronomic and economic damage. In the presentstudy, serological and molecular tests were done in order to analyze theORF3 coding for the coat protein (CP) and characterize the BYDV isolatesoccurring in Tunisia. For this purpose, barley samples, collected followinga North-South trend, were subjected to a DAS-ELISA using antibodiesagainst three variants of BYDV PAV, MAV and RPV. Results revealed thatthe South area is the most affected area and showed a prevalence of PAVvariant. Then, for positive samples, CP gene were amplified, cloned andsequenced. A total of six PAV isolates were identified and named PAV-TN1to PAV-TN6. Phylogenetic analysis showed that Tunisian isolates presenta strong similarity with the Moroccan isolate (PAV-MA9517),the Frenchisolate (PAV-FH1) and the German one (PAV-G) suggesting that the virusmay be transmitted by the same migration aphid.REF 396The Rapid Implementation of a Novel Sampling and Molecular TestingAlgorithm to Facilitate the Management of a Large Outbreak ofMeasles in South WalesCatherine MOORE 1 , Simon COTTRELL 2 , Jorg HOFFMANN 3 ,Michael CARR 4 , Rachel JONES 11 Wales Specialist Virology Centre, Public health Wales MicrobiologyCardiff, University Hospital of Wales, CARDIFF, UNITED KINGDOM;2 Public Health Wales Communicable Disease Surveillance Centre, Templeof Peace and Health, CARDIFF, UNITED KINGDOM; 3 Health ProtectionDivision (Mid and West Wales), Public Health Wales, SWANSEA, UNITEDKINGDOM; 4 National Virus Reference Laboratory (NVRL),UniversityCollege Dublin, DUBLIN, IRELANDVirologie, Vol 17, supplément 2, septembre 2013S229