rologie i - European Congress of Virology

5 th European Congress of Virologyupon general or regional anaesthesia on reactivation of HHV 6 and HHV7.58 patients undergoing long lasting (average 5.7 h) microvascular free flapsurgery were enrolled in this study. Anaesthesia methods that were appliedgeneral anaesthesia (GA) (n=35) or regional anaesthesia (RA) (n=23)depending of tissues defect and surgery planned donor place. Peripheralblood samples were collected before and 10 days after surgery, DNAisolated from whole blood and cell free blood plasma and viral genomicsequences detected by nPCR using corresponding primer pairs. Before thesurgery latent HHV 6 infection was detected in 13 and 6 patients with GAand RA, respectively but latent HHV 7 infection in 6 and 17 patients withRA and GA, respectively. Two patients in each group had active HHV 6infection, 3 patients with GA and 4 with RA – active HHV 7 infection.After surgery reactivation in GA group was observed in 7 patients (in onepatient HHV 6 and in 6 patients HHV 7, respectively). In the RA grouponly in one patient reactivation of HHV 7 was detected. After surgery reactivationof HHV 6 and HHV 7 occurs more frequent in patients to whomGA is applied (p