rologie i - European Congress of Virology

5 th European Congress of VirologyPCR positive respiratory samples, a standardized viral load (expressed asRSV RNA copies per 100 cells) was calculated using the kit Cell controlr gene ® Biomerieux. The Sofia FIA RSV displayed sensitivities of 78.8%(from 47.3% to 95%, in patients >2 years old and in infants, respectively)as opposed to PCR. The negative predictive value was 91.3% (from 89%to 96.6% in >2 years old and in infants, respectively). The cell loads in the123 respiratory specimens were between 3 and 5 Log10 cells per PCR. Nosignificant difference is observed in the cell loads in respiratory specimensfrom patients of different age groups. Nevertheless, the respiratory viralloads dramatically decreased in patients above 2 years of age. Sofia FIARSV ® is a sensitive and specific method with a high negative predictivevalue for patients of different age groups. Its performances prove equivalentto molecular tests in infants throughout the RSV circulation perioddue to high respiratory viral loads in this group. This fact can be explainedby the quasi systematic RSV primo infection before the age of 2 years.Methods: Nasopharyngeal swabs were prelevated from patients with acuterespiratory illness admitted from November 2012 to March 2013. Thesamples were tested with xTAG Respiratory Virus Panel Fast (Luminex).Results: A total of 227 samples were received, most of them (77.3%) frompediatric (0 14 years old) patients. More than half (56.3%) of the sampleswere positive for at least one virus, the predominant viruses being influenzaA and influenza B. Adenovirus, RSV and Rhinovirus/Enterovirus (RV/EV)were detected most frequently in children less than 5 years old. Elevensamples (8.5%) were positive for two viruses and one sample was positivefor three viruses. Co infection was significantly associated (p=0.02)with age under 5 years. Adenovirus and RV/EV were more frequentlydetected with other viruses, and all the viruses of the panel (influenza,parainfluenza, RSV, adenovirus, RV/EV, Coronavirus, Metapneumovirus,Bocavirus) were identified in co infections.Conclusion: Co infections with two or more respiratory viruses are oftendetected in pediatric patients with acute respiratory illness.REF 524The role of newly discovered viruses in the development of lowerrespiratory tract infections in KuwaitSahar ESSA 1 , Haya ALTAWALAH 1 , Abdulla OWAYED 2 , MossaKHADADHA 3 , Nasser BEHBEHANI 3 , Widad AL NAKIB 11 Department of Microbiology/Faculty of Medicine/Kuwait University,Kuwait, KUWAIT; 2 Department of Pediatrics/Faculty of Medicine/Kuwaituniversity, Kuwait, KUWAIT; 3 Department of Medicine/Faculty of Medicine/Kuwaituniversity, Kuwait, KUWAITIntroduction: More than 80% of the cases of respiratory tract infectionsare viral in origin. Although mostly causing self limited upper respiratorytract infections (URTI), they can be associated with serious lower respiratoryinfections (LRTI) with a high level of morbidity, particularly inchildren. Little is known about the role of newly discovered viruses in thedevelopment of URTI and LRTI in Kuwait and the Gulf region. Methods:The aim of this study is to investigate the role of seven newly discoveredviruses which include coronaviruses (HCoV) NL63, OC43, and 229E,human metapneumovirus (hMPV), bocavirus, human polyomaviruses WU(WUV) and KI (KIV) by using sensitive molecular techniques.Results: Seven hundred thirty five hospitalized patients were screenedduring three and a half year period, from September, 2010 to April, 2013.Of the 285 patients with viral respiratory infection HCoV OC43 was detectedin twenty five patients (8.8%), both HCoV 229E and bocavirus weredetected in fourteen patients (4.9%), hMPV in fifteen patients (5.3%),WUV in ten patients (3.8%), KIV in four patients (1.4%), and HCoVNL63, was not detected in any of our patients’ samples.Conclusion: We conclude that newly discovered viruses do play a rolein the development of both URTI and serious LRTI in Kuwait. Rapididentification of viral infections can help control nosocomial transmission,reduce overall antibiotic use and improve the treatment and managementsof these infections.REF 525Multiple respiratory viral co infections in patients with acute respiratoryinfectionDragos FLOREA 1 , Angelica VISAN 1,2 , Anca DRAGANESCU 1 ,George JUGULETE 1,2 , Monica LUMINOS 1,2 , Dan OTELEA 11 National Institute for Infectious Diseases “Matei Bals”, Bucharest,ROMANIA; 2 University of Medicine and Pharmacy “Carol Davila”,Bucharest, ROMANIAObjective: the aim of the study was to investigate the prevalence of respiratoryviruses and the rate of viral co infection in patients hospitalizedwith acute respiratory infection in “Matei Bals” Institute.REF 526High proportion of multiple infections with respiratory viruses inyoung children with acute respiratory tract infectionsAtsushi KAIDA 1 , Hideyuki KUBO 1 , Nobuhiro IRITANI 1 , Koh IchiTAKAKURA 1 , Jun Ichiro SEKIGUCHI 1 , Minori OHYAMA 1 , UraraKOHDERA 2 , Masao TOGAWA 3,4 , Kiyoko AMO 3 , Masashi SHIOMI 3 ,Seiji P YAMAMOTO 1 , Kaoru GOTO 1 , Atsushi HASE 1 , TsutomuKAGEYAMA 51 Osaka City Institute of Public Health and Environmental Sciences,Osaka, JAPAN; 2 Nakano Children’s Hospital, Osaka, JAPAN; 3 OsakaCity General Hospital, Osaka, JAPAN; 4 Osaka City Sumiyoshi Hospital,Osaka, JAPAN; 5 Influenza Virus Research Center, National Institute ofInfectious Diseases, Tokyo, JAPANObjectives: Respiratory virus is a major etiologic agent of acute respiratorytract infections (ARTIs) in young children. This study was conductedto detect multiple respiratory viruses and to analyze their co infection andmutual association using statistical analysis.Methods: During January 2010 – December 2011, 1,044 respiratory clinicalspecimens were obtained from children (< 6 years of age) with ARTI.To detect viral genes, multiplex real time PCR was conducted for respiratoryviruses (human metapneumovirus [hMPV], respiratory syncytial virus[RSV A, B], human parainfluenza virus types 1–4 [HPIV 1–4], humanbocavirus 1 [HBoV 1], human coronavirus [229E, OC43, HKU1, NL63],influenza virus [A, A (H1N1)2009, B, C], human adenovirus [HAdV],human enterovirus, and human rhinovirus [HRV]).Results: The 1,414 viruses were detected from 891 (85.3%) specimens,of which 388 (43.5%) specimens were multiple virus positive (2 viruses,279;=3 viruses, 109). The most detected was HRV (n=390). The proportionof multiple virus positives was higher in the 0–35 month age group. Thecombinations of HPIV 1 and HPIV 3, HPIV 1 and RSV A, HPIV 3 andhMPV, hMPV and RSV A, hMPV and FLUA (H1N1) 2009, RSV A andRSV B, and HRV and FLUA (H1N1) 2009 were negatively correlated. Incontrast, HAdV and HBoV 1 were positively correlated.Conclusions: Young children are highly susceptible to respiratory viruses.Viral co infections are common.REF 527Respiratory viruses in patient with acute respiratory tract infectionsin AYDIN, TURKEYSevin KIRDAR 1 , Neriman AYDIN 1 , Ayse YENIGUN 2 , EmelCEYLAN 31 Adnan Menderes University, Department of Medical Microbiology,Aydin, TURKEY; 2 Adnan Menderes University, Department of PediatricDiseases, Aydin, TURKEY; 3 Adnan Menderes University, Department ofPulmonary Medicine, Aydin, TURKEYS266 Virologie, Vol 17, supplément 2, septembre 2013