rologie i - European Congress of Virology

5 th European Congress of VirologyREF 553The prevalence of DNA HSV 1 and HSV 2 in Polish patients subjectedto allogeneic hematopoietic stem cell transplantationSylwia RYNANS 1 , Agnieszka TOMASZEWSKA 2 , TomaszDZIECIATKOWSKI 1,3 , Katarzyna MOCKO 4 , Anna MAJEWSKA 1 ,Maciej PRZYBYLSKI 1,3 , Kazimierz HALABURDA 5 , GrazynaMLYNARCZYK 11 3Medical University of Warsaw, Chair and Department of Medical Microbiology,Warsaw, POLAND; 2 Institute of Hematology and TransfusionMedicine, Department of Haemopoetic Stem Cell Transplantation, Warsaw,POLAND; 3 2Central Clinical Hospital in Warsaw, Department ofMicrobiology, Warsaw, POLAND; 4 Warsaw University of Life Sciences,SGGW, Interfaculty Department of Biotechnology, Warsaw, POLAND;5 Medical University of Warsaw, Department of Hematology, Oncologyand Internal Medicine, Warsaw, POLANDHerpes simplex viruses type 1 (HSV 1) and 2 (HSV 2) belong to widespreadcontagious factors. A situation might becomes dangerous whenpatient is subjected to hematopoietic stem cells transplantations (HSCT)with the profound immunosuppression. A retrospective review of serasamples coming from collection of Department of Microbiology fromgroup of 54 adult recipients of allogeneic HSCT was made. All ofthem received anti viral prophylaxis, using oral form of acyclovir. Serumsamples taken before transplant were examined for presence of specificanti HSV 1 and anti HSV 2 antibodies in IgM and IgG classes. After transplantationquantitative real time PCR was used to determine viral load inplasma samples in range 0 100 days. Achieved results showed that antibodiesdirected against the HSV 1 in IgG class were appearing in the 87%examined persons and anti HSV 2 IgM antibodies in 3% patients. ViralDNA was detected in plasma samples in 15 (27,8%) of the 54 recipients,mostly between day 21st and day 45th of transplantation. All of themdeveloped fever of unknown origin, and over 50% had GvHD features.All of positive patients have HSV 1 viremia; none of HHV 2 DNA wasdetected. There is a high frequency of detectable HSV 1/2 viral load inalloHSCT recipients and it may lead to an increased risk for fatal symptomaticdisease. The availability of quantitative real time PCR method meansthat results are available in a clinically helpful time frame, which shouldassist with implementing timely therapeutic intervention and assessingresponse to treatment.REF 554Use of multiplex real time PCR assay for detection and differentiationviral haemorrhagic cystis in patients undergoing allogeneichaematopoietic stem cell transplantationSylwia RYNANS 1 , Tomasz DZECIATKOWSKI 1,2 , MaciejPRZYBYLSKI 1,2 , Agnieszka TOMASZEWSKA 3 , KazmierzHALABURDA 4 , Grazyna MLYNARCZYK 11 Medical University of Warsaw, Chair and Department of MedicalMicrobiology, Warsaw, POLAND; 2 Central Clinical Hospital in Warsaw,Department of Microbiology, Warsaw, POLAND; 3 Institute of Hematologyand Transfusion Medicine, Department of Haemopoetic Stem CellTransplantation, Warsaw, POLAND; 4 Medical University of Warsaw,Department of Hematology, Oncology and Internal Medicine, Warsaw,POLANDHaemorrhagic cystitis (HC) is characterized by painful haematuria dueto haemorrhagic inflammation of the urinary bladder mucosa. This complicationin group of allogeneic haematopoietic stem cell transplantation(HSCT) is associated with the reactivation of urotropic viruses, such ashuman adenoviruses (HAdV) and polyomavirus BK (BKV). The majoraim of this study was to develop a duplex real time PCR assay for thesimultaneous detection of HAdV and BKV using TaqMan hydrolyzingprobes. Second aim was a retrospective review of group of 64 adult recipientsof allogeneic HSCT with history of haemorrhagic cystitis. 92 serumand 141 urine samples were examined for presence of HAdV/BKV DNA,using quantitative real time PCR to determine viral load in range 40 80days after HSCT. Viral DNA was detected in the sera samples of 31 persons(48%): BKV in 9 (14%) and HAdV in 22 (34%). Viruria was detected in:BKV in samples taken from 24 (37,5%) and HAdV from 55 (86%) individuals,showing symptoms of late haemorrhagic cystitis. None of describedindividuals died during detectable viremia period. Obtained quantitativeresults usually were at low to medium level, placed between 100-1500copies/ml for sera and 400-18000 copies/ml for urine samples, respectively.There is a high frequency of detectable HAdV viremia in HSCTrecipients with signs of haemorrhagic cystitis. Furthermore, establishmentof appropriate procedures for monitoring active HAdV/BKV infection isimportant to clarify the virus infection in transplant recipients.REF 555Cytomegalovirus and human herpesvirus 7 co infections in Polishadults subjected to allogeneic haemopoietic stem cell transplantationsSylwia RYNANS 1 , Agnieszka TOMASZEWSKA 2 , TomaszDZIECIATKOWSKI 2,3 , Anna KRYSKO 4 , Maciej PRZYBYLSKI 2,3 ,Karolina PIEKARSKA 5 , Kazimierz HALABURDA 6 , GrazynaMLYNARCZYK 11 Medical University of Warsaw, Chair and Department of Medical Microbiology,Warsaw, POLAND; 2 Institute of Hematology and TransfusionMedicine, Department of Haemopoetic Stem Cell Transplantation, Warsaw,POLAND; 3 Central Clinical Hospital in Warsaw, Department ofMicrobiology, Warsaw, POLAND; 4 Warsaw University of Life Sciences– SGGW, Interfaculty Department of Biotechnology, Warsaw, POLAND;5 Medical University of Warsaw, 2nd Faculty of Medicine, Warsaw,POLAND; 6 Medical University of Warsaw, Department of Hematology,Oncology and Internal Medicine, Warsaw, POLANDHuman herpesvirus 7 (HHV 7) is widespread around the world and mayalso be a possible cofactor for cytomegalovirus (CMV) infection in haematopoieticstem cell transplant recipients (HSCT). In case of viral diseaseswhere specific treatment is available, real time PCR assays constitutereliable diagnostic tools enabling timely initiation of appropriate therapy.The presence of CMV and HHV 7 was confirmed by the detection of DNAisolated from 1027 plasma samples. A group of 69 HSCT recipients wasexamined in early post transplant period using quantitative real time PCR.Within the study period, 62% of patients had at least once CMV DNAemia, while HHV 7 DNA was found in 43% of subjects. Co infection wasdetected in the plasma samples collected from 18 patients (26%). Patientswith concomitant HHV 7 DNA emia had significantly higher number ofCMV DNA copies compared with those without HHV 7 infection (1986vs. 432 copies/ml) but there was no difference in duration of CMV DNAemia between these groups. On the other hand, while the load of HHV 7DNA was comparable between patients with CMV DNA emia and withoutCMV DNA emia, the duration of HHV 7 DNA emia was significantlylonger in the first group (38.5 vs. 14 days). HHV 7 DNA emia is veryfrequently detected in Polish HSCT recipients. In those, who have subsequentCMV reactivation, the coexistence of the viruses may negativelyaffect the kinetics of infection with either of them. Therefore the investigationof concomitant HHV 7 DNA emia could affect the prognosis ofpost transplant patients suffering from CMV reactivation.REF 556Effect of a TNF alpha antagonist based therapy on the VZV specificT cell immunity in a cohort of psoriatic patientsAlda SALDAN 1 , Elena SANDINI 2 , Daniel TINTO 1 , StefanoPIASERICO 2 , Andrea PESERICO 2 , Giorgio PALÙ 1 , Davide ABATE 11 Department of Molecular Medicine, University of Padova, Padova,ITALY; 2 Dermatology Unit, Padova General Hospital, Padova, ITALYIntroduction: biological antagonists of TNF (etanercept, adalimumab)are currently used for the treatment of severe psoriasis. One of theS274 Virologie, Vol 17, supplément 2, septembre 2013