rologie i - European Congress of Virology

5 th European Congress of Virologyserotypes and the close genetic relationship between them leads to potentialcross species infections. Serotype I canine strain (CCoVI) differs by thepresence of a unique open reading frame ORF3, coding for an accessoryglycoprotein gp3, with unknown function. In a phylogenetic study performedto characterize Coronavirus infected cats, structural genes S, M and Nwere sequenced and allowed to detect a new recombinant FCoVI/CCoVIstrain on 5 cats out of 26 samples. In these strains, the S gene clusters withFCoVI type, whereas downstream genes ORF3, M and N are grouped withCCoVI genotype. Moreover, ORF3 found in cats samples, displayed oneor two identical deletions, never described in canine samples. In a possiblecross species virus transmission context, our aim is to understand the roleof gp3 and determine if the deletions observed play a role during the adaptationof the recombinant canine strain to the feline species. More globally,this study will give an overall view of molecular mechanisms implied inthe inter specific host transmission of Coronaviruses.Acute gastroenteritis is one of the most common diseases in humans aswell as farm animals. Small, non enveloped RNA viruses are recognizedas important causes of this disease. The objective of the study was to determinethe prevalence and genetic diversity of members of genera Sapovirus,Kobuvirus, and Mamastrovirus in asymptomatic pigs in the Czech Republic.A total of 196 fecal samples were tested by reverse transcriptionPCR (RT PCR) assay using gene specific primers. Phylogenetic analysiswas carried out using the neighbor joining method with a bootstrap testof 1000 replicates using MEGA software package v4.0. The most prevalentvirus was kobuvirus (87.3%), followed by astrovirus (34.2%) andsapovirus (10.2%). Statistically significantly highest number of positivesamples was detected in the age group of post weaning pigs. Consideringthe possible zoonotic potential of monitored viruses, their geneticdiversity and degree of similarity to human strains was also determined.In conclusion, the circulation of porcine sapoviruses, kobuviruses, andastroviruses in the Czech Republic was shown thus extending their Europeandistribution. The high prevalence rate of gastroenteritis producingviruses in clinically healthy pigs confirmed the role of pigs as a reservoirof a heterogeneous viral population representing a constant threat tohuman health. The study was supported by the Ministry of Agricultureof the Czech Republic (Grant No. QH81061) and the Ministry of Education,Youth and Sports of the Czech Republic (Admirevet; Grant No.CZ.1.05/2.1.00/01.0006, ED0006/01/01).REF 331Prevalence and genetic diversity of porcine bocavirus 1 in apparentlyhealthy pigs in the Czech RepublicJana PRODELALOVAVeterinary Research Institute, Brno, CZECH REPUBLICBocaviruses (family Parvoviridae) are pathogens causing various diseasesin both humans and animals. Porcine bocavirus 1 (PBoV1) was firstlyidentified in Sweden in 2009 and it is potentially associated with swinedisease. The virus was detected in pigs suffering from porcine circovirusassociated disease and respiratory disease but also from clinically healthypigs. The aim of the present study was to detect and measure the prevalenceof PBoV1 and analyse partial PBoV1 sequences. Faecal samples were collectedfrom different farms located in the South Moravia region, tested byPCR specific for VP1/VP2 gene of PBoV1 and sequenced. Among 179samples obtained from apparently healthy pigs of different age groups, theprevalence was 22.4%. Higher infection rate was found in post weaningpigs (75.5%, 34/45) compared with finisher pigs (8.2%, 5/61) and saws(3%, 1/33). No PBoV1 positive samples were detected in nursing piglets(0/40). PBoV1 positive samples were obtained from three out of eightfarms. By comparative sequence analysis of 496 bp fragments of VP1/VP2gene from domestic pigs and sequences available in GenBank we detectedonly minor differences between PBoV1 s. This result indicates thatPBoV1 s circulating within the apparently healthy domestic pig populationin South Moravia region were highly similar. This work was supportedby the Ministry of Agriculture of the Czech Republic (Grant No. NAZVQH81061) and the Ministry of Education, Youth, and Sports of the CzechRepublic (Grant No. AdmireVet CZ.1.05/2.1.00/01.006, ED006/01/01).REF 333Serological surveilance for selected viral agents in ROE DEER(Capreolus Capreolus) and RED DERR (Cervus Elaphus) in CroatiaBesi ROIC, Lorena JEMERSIC, Svjetlana TERZIC, Andreja JUNGIC,Dragan BRNIC, Tomislav KEROS, Jelena PRPICCroatian Veterinary Institute, Zagreb, CROATIADuring the hunting season of 2009, blood samples from 32 roe deer(Capreolus capreolus) and 55 red deer (Cervus elaphus) were collectedin four Croatian regions. Sera were tested by enzyme linked immunosorbentassay (ELISA) for the presence of antibodies against bovine herpesvirus 1 (BHV 1), parainflurnza 3 virus (PI 3), bovine respiratory syncytialvirus (BRSV), bovine viral diarrhea virus (BVDV) and bluetonguevirus (BTV). Specific antibodies were detected for bovine herpes virus1 (BHV 1) in 12.50% (4 positive/32 tested) roe deer and in 25.45% (14positive/55 tested) red deer. Additionally, positive results were obtainedagainst parainfluenza 3 virus (PI 3) in 68.75% roe deer (22 positive/32 tested)and 83.63% red deer (46 positive/55 tested). Most of the seropositivesera were recorded among red deer in the eastern part of Croatia which isknown as a district with high density populations of wildlife species. Noantibodies were detected against any of the other viral agents assayed inthis study. The presence of BHV 1 and PI 3 virus antibodies indicate thatboth deer species have been exposed and have responded serologically toviral agents which are related or identical to the domestic livestock pathogens.This study provides the first survey for the presence of antibodies toviral diseases in cervids from Croatia.Key words: roe deer (Capreolus capreolus), red deer (Cervus elaphus),viral disease, serology, CroatiaREF 332Prevalence and genetic diversity of porcine sapoviruses, kobuviruses,and astroviruses in asymptomatic pigs in the Czech RepublicLucie DUFKOVA 1 , Ivana SCIGALKOVA 2 , Romana MOUTELIKOVA 1 ,Hana MALENOVSKA 1 , Jana PRODELALOVA 11 Veterinary Research Institute, Brno, CZECH REPUBLIC; 2 Faculty ofScience/Masaryk University, Brno, CZECH REPUBLICREF 334Molecular characterization of VP2 Gene of infectious pancreaticnecrosis virus from Mexican isolatesEdith ROJAS ANAYA 1 , Elizabeth LOZA RUBIO 1 , Celene SALGADOMIRANDA 1,2 , Gary GARCIA ESPINOSA 21 INIFAP, CENID Microbiología Animal, México City, MÉXICO; 2 FMVZ,UNAM, México City, MÉXICOInfectious pancreatic necrosis virus (IPNV), is considered one of the mostimportant viral pathogen in salmonids and remains a serious problem inthe aquaculture industry. To better understand the genetic and biologicaldiversity of these viruses in Mexico it is necessary to further study thefeatures which include genetic characterization and virulence that mayimpact on the production of biologicals. The aim of this study was toperform the molecular characterization of the VP2 gene Mexican IPNVisolates from different geographical regions. Seven isolates were obtainedfrom rainbow trout hatchery, during 2006 and 2007 of the major farmsproducing trout: Michoacan, Puebla and State of Mexico. We designedS212 Virologie, Vol 17, supplément 2, septembre 2013