rologie i - European Congress of Virology

5 th European Congress of Virology(PEGE) from Genova (Italy), who had his vacation in Corsica and in SouthernFrance at the end of August begining September 2011. He boughtfigatelli in a supermarket of Corsica and he stored them at +4 ◦ C untiluse (21 September 2011). During the last week of october he presenteda progressive outset of fever, fatigue, loss of appetite and he wasadmitted to the Infectious Disease Service with a provisional diagnosisof nonA nonC acute hepatitis. Anti hepatitis E virus IgM and IgGwere positive in ELISA assay (Radim, Italy). Molecular analysis showedthat the HEV strain belonged to genotype3.Comparison of the PEGEORF2 region against 98 GT3 sequences isolated in pig, wild boar orhuman revealed that PEGE sequence was closest related to GQ426992sequence belonged to a patient from Marseille (France), with an identityof 97.7%. PEGE was 98.3% similar to an second human strain (Fr 08V22)isolated in an other locality of Southern France. In contrast PEGE wasonly 78.6 91.7% similar to the human and swine HEV strains isolated inItaly. In this contest the possibility that PEGE was infected with a frenchHEV strain by ingesting contaminated food seems the most plausiblehypothesis.Conclusion:Although hepatitis E is mainly an authochthounousdisease in Europe, such as epidemiological feature is significant toPublic Health, imported infection should be considered also in patientswith acute hepatitis symptoms,which traveled in developed countries.Thiswork was partially supported by the European Union Seventh FrameworkProgramme [FP7/2007 2013] under Grant Agreement n o 278433PREDEMICS.REF 364Dengue Diagnosis and Epidemiology by a Regional Reference Laboratoryin 25 years, Rio de Janeiro, Brazil: an overviewFlavia DOS SANTOS, Ana Maria DE FILIPPIS, Eliane ARAUJO,Monique LIMA, Fernanda NOGUEIRA, Nieli FARIA, JaquelineSIMOES, Simone SAMPAIO, Priscila NUNES, Bianca GONÇALVES,Manoela HERINGER DA SILVA, Clarice RODRIGUES, Carla SOUSA,Dinair LIMA, Rita Maria NOGUEIRAOswaldo Cruz Institute, Fiocruz, Rio De Janeiro, BRAZILDengue activity in Brazil has been evidenced by a large number of cases.DENV 1, DENV 2 and DENV 3 were introduced in Rio de Janeiro, in1986, 1990 and 2000, respectively. In 2010, DENV 4 reemerged after28 years. DENV 1 caused an explosive epidemic in 1986 1987, DENV2 introduction in 1990 caused the first DHF/DSS cases. DENV 3 causedan epidemic in 2002 with the largest number cases. In 2008, thecountry experienced the most severe epidemic in terms of morbidity andmortality in children. Since 1986, the laboratorial diagnosis has playedan important role in the surveillance and epidemiology. Virus isolationand IgM ELISA were first used. After DENV 2 introduction in 1990,the immune response characterization performed by the heamaglutinationinhibition test (HI), was replaced by an IgG ELISA. In the 90 ′ s,the RT PCR and sequencing were used for nucleic acid detection andcharacterization. NS1 capture tests were used for the early diagnosis ofdengue after 2007. A total of 47,346 suspected cases were received bythe Laboratory of Flavivirus, IOC/FIOCRUZ, RJ, Regional ReferenceLaboratory for the Brazilian Ministry of Health, from 1986 to 2011. Atotal of 32,374 cases were tested by MAC ELISA, 25,037 were submittedto virus isolation, and 829 cases to IgG ELISA. The RT PCR was performedin 7,441 cases and NS1 ELISA in 1,124.Virus isolation and RTPCR identified the serotype in 4,990 cases. The implementation of newtechniques over the years constituted an important and reliable tool forthe disease surveillance in Brazil. Support by CNPq FAPERJ PAPES VIFIOCRUZ MSREF 365Molecular and epidemiological profiles of Hepatitis C virus genotype4 in Regione Lazio, ItalyAnna Rosa GARBUGLIA 1 , Daniele LAPA 1 , Catia SIAS 1 , AngelaTESTA 2 , Maria Rosaria CAPOBIANCHI 11 Laboratory of Virology, National Institute for Infectious Diseases “LazzaroSpallanzani, Rome, ITALY; 2 Clinical Departement, National Institutefor Infectious Diseases “Lazzaro Spallanzani”, Rome, Italy, Rome, ITALYIn Europe HCVgenotype 4 (GT4)shows an increasing prevalence. Thisprevalence is particularly high in Italy,and reaches values of 8,4% inspecific areas. Among patients(Pt) attending INMI L Spallanzani clinicalsetting we observed a prevalence of 13%.The goal of this study isto provide a picture of GT4 strains circulating in Regione Lazio. GT4subtypes were determined by direct sequencing of a PCR amplified productsfrom NS5B and core regions.The phylogenetic tree was inferred bythe Neighbor Joining(NJ) method with bootstrap values based on 1,000replicates.Sixty seven Pt were included in the analysis;46 were italian and21 were linving in Rome,but they were born abroad.The mean age was45years(range 18 71). Among italian Pt we found 42 GT4d, 3 GT4a and1 GT4r, never described in Italy. Among foreign Pts 11 people harbouredGT4a, GT4d was observed in 8 Pt(38%), most of which were drug addicts.GT4c and o were identified into 2 foreign Pts.The average pairwise nucleotideidentity within the entire group of GT4d patients strains was 95.8%in NS5B region. Three italian Pts showed the same sequences. The pairwisenucleotide identity within the entire group GT4a was 90.6%. A 100%concordance was found between the the subtype assignement in the NS5Band core region, implying no evidence of recombination. The phylogenetictree indicated 2 distinct monophyletic clades for GT4a and GT4d.The 4d strains isolated in foreign Pt were grouped in the same clusters ofitalian 4d strains.New variants had been found.Conclusion:The significativeprevalence of GT4d among immigrated Pt drug users suggests needleexchange as main route of transmission in this population. The study indicatesthe introduction of new subtypes and existence of GT4variants inItaly. Clinical research and drug development program must be stimulatedto further deter the spread of GT4.REF 366Seroepidemiology and molecular epidemiology of enterovirus 71 inRussiaLudmila AKHMADISHINA 1 , Tatiana EREMEEVA 1 , OlgaTROTSENKO 2 , Olga IVANOVA 1 , Mikhail MIKHAILOV 1 , AlexanderLUKASHEV 11 Chumakov Institute of Poliomyelitis and Viral Encephalitides, Moscow,RUSSIA; 2 Khabarovsk Institute of Epidemiology and Microbiology, Khabarovsk,RUSSIAEnterovirus 71 (EV71) is an emerging human pathogen causing massiveepidemics of hand foot and mouth disease (HFMD) with occasionalsevere neurological complications in Asia. EV71 also circulates in Europe,however it does not cause large outbreaks. The reason for distinct epidemiologicalpatterns of EV71 infection in Europe and Asia remainunknown. In six regions of Russian Federation, seroprevalence of EV71ranged from 5% to 20% in children aged 1 2 years and from 19% to 83% inchildren aged 3 5 years. There was no correlation between EV71 seroprevalenceand income level and population density. In the European regionsof Russia seroprevalence among children aged 3 5 years was 21 27%.Significantly higher rates were observed in Eastern regions of Russia withVirologie, Vol 17, supplément 2, septembre 2013S221