rologie i - European Congress of Virology

5 th European Congress of VirologyREF 298Antiviral activity of extracts of bacteria isolated from soils moundsagainst Feline herpesvirus (FeHV 1) and Feline calicivirus (FCV)Marina PADILLA, Luciana K. KHON, Claudia Beatriz MENEZES,Fabiana FANTINATTI GARBOGGINI, Matheus MARTINI, AnaCaroline BARNABÉ, Ricardo DURÃES CARVALHO, LeonardoCASERTA, Juliana SANTIAGO, Clarice ARNSUNICAMP, Laboratory of Virology, Campinas, BRAZILThis study demonstrated the antiviral properties of extracts from bacteriaisolated from soils mounds in Brazilian northeast against feline herpesvirus(FeHV 1) and feline calicivirus (FCV). Microorganisms isolatedfrom land of termite were incubated in culture medium for four weekat 30 ◦ C and these inoculums were extracted by liquid liquid extractionwith ethyl acetate. Antiviral activity was measured by virus titration techniqueand calculated the percentage of viral inhibition (PI) for with virustreat with samples. The 50% effective concentration (EC50), 50% cytotoxicconcentration (CC50), selectivity index (SI) were determined usingthe MTT method. The objective of this study was to evaluate in vitro 16microorganism extracts for antiviral activity against FeHV 1 and FCV.The extracts were considered active when PI is higher than 90%. Threeextracts were active against FeHV 1: LC04 Ac and MC25 met with 97%of inhibition and LC22 CM with 99% inhibition and against FCV werealso three active extracts: LC16 Ac and LC22 CM with 99% of inhibitionand LC16 met with 98% inhibition. This active extracts were evaluated inMTT assay to calculate the selectivity index. The LC04 Ac and MC25 metshow SI considered promising equal to 16.58 and 6.23 respectively againstFeHV 1. For the FCV the promising value of SI were found to LC16 Acand LC22 CM extracts with SI values equal to 12.59 and 11.00, respectively.These results shows that bacteria isolated from soils mounds have apotential source for discovery of new antiviral drugs.REF 299The Serological, Virological and Pathological Investigations of RiftValley Fever Virus Infection in Aborted Cattle, Sheep, Goats and TheirFoetusesKamil ATLI 5 , Mehmet KALE 1 , Sibel HASIRCIOGLU 1 , OzlemOZMEN 2 , Nuri MAMAK 3 , Sibel GUR 4 , Orhan YAPICI 6 , SibelYAVRU 5 , Mehmet HALIGUR 2 , Oya BULUT 51 University of Mehmet Akif Ersoy, Faculty of Veterinary Medicine, Departmentof Virology, Burdur, TURKEY; 2 University of Mehmet Akif Ersoy,Faculty of Veterinary Medicine, Department of Pathology, Burdur, TUR-KEY; 3 University of Mehmet Akif Ersoy, Faculty of Veterinary Medicine,Department of Internal Medicine, Burdur, TURKEY; 4 University of AfyonKocatepe, Faculty of Veterinary Medicine, Department of Virology, Afyon,TURKEY; 5 University of Selcuk, Faculty of Veterinary Medicine, Departmentof Virology, Konya, TURKEY; 6 University of Kyrgyzstan TurkeyManas, Faculty of Veterinary Medicine, Department of Virology, Bishkek,KYRGYZSTANThis study was investigated the serological, virological and pathologicalof Rift Valley Fever Virus (RVFV) infection in aborted cattle (Holstein;178 pcs), sheep (Domestic of breed; 160 pcs), goat (Hair goat; 66pcs,Honamli goat; 98pcs, Saanen goat; 16pcs) aborted cattle foetuses (8pcs), sheep foetuses (24 pcs), goat foetuses (5pcs). Samples werecollected between July 2009 to September 2010. Blood serum sampleswere collected from aborted cattle, sheep and goats were detected asseropositive for 7 cattle (3.93%), 4 sheep (2.50%) and 18 goats (10.0%)by indirect ELISA. Distribution of according to breeds in determined of18 seropositive goats, 13 Hair goat (19.70%), 2 Honamli goat (2.04%)and 3 Saanen goat (18.75%) was observed. Blood serum samples werecollected from aborted cattle foetuses, sheep foetuses and goat foetuseswere detected as seropositive for 1 cattle (12.50%), 4 sheep (16.67%),1 goat (20.0%). While cytopathic effect (CPE) was not observed in anyof them when leukocyte samples that were collected from aborted cattleand sheep, were inoculated in Vero cell cultures. CPE observed positiveonly 2 (1.11%) goat leukocyte samples. Also, CPE observed as positive3 sheep (12.50%), 1 goat (20.0%) when leukocyte samples that werecollected from aborted sheep&goat foetuses. Liver, spleen and brainsamples collected from aborted cattle, sheep and goat foetuses were notdetermined for RVFV antigen by IP and IF tests. As a result, RVFVinfection was not detected in the Western Mediterranean, Turkey.Key words: Abortion, Pathology, RVFV, Serology, VirologyREF 300Experimental infection of European breed sheeps with 2 differentvirulent strains of Rift Valley fever virusSandra LACÔTE 1 , Marie MOROSO 1 , Pierre SARRADIN 2 , MichelPEPIN 3 , Isabelle SCHWARTZ 4 , Philippe MARIANNEAU 11 Unité Virologie, ANSES Laboratoire de Lyon, Lyon, FRANCE; 2 UE1277Plate forme d’Infectiologie expérimentale, INRA, Nouzilly, FRANCE;3 USC INRA/VAS 1233, équipe pathogènes émergents et rongeurs sauvages,Lyon, FRANCE; 4 VIM, INRA, Jouy en Josas, FRANCERift Valley Fever (RVF) is a mosquito borne disease occurring in humanand animals, mostly in livestock. Outbreaks of severe disease occurredthroughout Africa and more recently in the Arabia peninsula. This disease,resulting in « abortion storms » and with 20% of mortality in ruminants,is caused by a virus called RVF virus (RVFv), belonging to the Bunyaviridaefamily and to the Phlebovirus genus. Human infection results infebrile disease with 1 2% of more severe disease. Improving knowledgeon the susceptibility and pathology induced in European sheep breeds byRVFv and developing effective and safe diagnosis tools and vaccines areimportant in the hypothesis of a future emergence of the disease in Europe.We have characterized the clinical symptoms, the viral replication and theimmune and inflammatory responses developed by 5 month old lambs(Ile de France breed) infected by two different virulent strains of RVFv.Few days after inoculation, lambs developed a febrile disease with hyperthermiacorrelated with viral replication. Humoral immune response wasstudied by following IgM and IgG antibodies production and high titers ofneutralizing antibodies were detected. This successful infection of Ile deFrance sheep by RVFv illustrates the potential risk for disease importationand shows that this model is useful to study transmission, viral cycle ornew vaccine candidates.REF 301Detection and genetic analysis of group C rotaviruses in pigs in theCzech RepublicRomana MOUTELIKOVA, Jana PRODELALOVA, Lucie DUFKOVAVeterinary Research Institute, Brno, CZECH REPUBLICThe objective of our work was to examine epizootiological situation in theCzech pig farms with regard to group C rotaviruses (RV C). We examined aset of total 293 porcine faecal samples or gut contents gathered from sevendifferent farms. The RT PCR detection of double stranded (ds) RNA of RVC was carried out with the use of primers specific for the gene coding proteinVP6. In the set of 220 stool samples from different age groups of pigsa total of 47 (21.4%) was positive for RV C dsRNA. The biggest portionof RV C positive samples was found among specimens from weaning andpost weaning piglets (35%). In the set of 73 specimens from gut content offinisher pigs collected at the abattoir 28 samples (38.4%) were positive forRV C. There are only limited numbers of investigations on porcine RV Cin Central Europe; many questions remain unanswered in respect of theirgenetic heterogeneity as well as their possible genetic relatedness withhuman strains. In the present study, we determined the near full lengthnucleotide sequences of VP4, VP6 and VP7 genes of selected porcine RVC strains. These sequences were aligned with each other and also withS204 Virologie, Vol 17, supplément 2, septembre 2013