rologie i - European Congress of Virology

5 th European Congress of VirologyREF 531Comparison of Idaho Tecnology FilmArray ® Respiratory Virus paneland Clart ® Pneumovir Microarray Genomica for multiplexed detectionof respiratory viruses in hospitalised patientsAla NASSEREDDIN, Nikolai KIRKBY, Claus BOHN CHRISTIANSEN1 Department of Clinical Microbiology, Rigshospitalet, Copenhagen,DENMARK; 2 Department of microbiology, Rigshospitalet, Copenhagen,DENMARK; 3 Department of microbiology, Rigshospitalet, Copenhagen,DENMARKObjective: To evaluate the clinical performance of two microarray systemsfor detection of respiratory viruses in hospitalised patients. Study design:In this prospectively study (February 4 to March 27, 2013) routine sampleswere included. Samples with inconclusive result in one of the assays wereexcluded. Only the first sample from each patient was included. Methods:CLART PneumoVir: RNA/DNA were extracted with EasyMac. Fragmentsof the viral genomes were amplificated by RT PCR and analysed. The FilmArrayRV integrates extraction, amplification, detection, and analysis in apouch. Viral targets: Influenza A/B, RSV, HMPV, Adenovirus, Rhinovirus,Enterovirus, PIV, Bocavirus and Coronavirus.Results: 121 samples were evaluated. Five samples gave inconclusiveresults in one of the assays. In 82/116 samples (70,6%) at least one viruswere detected in one or both assays. In 51 samples (43,9%) identical resultswere found (43 one virus, 7 two viruses and 1 three viruses). In 34 samples(29,3%) a negative result were found in both assays.In 12 samples (10,3%) FilmArray RP detected additional viruses. In 2samples (1,7%) PneumoVir detected additional viruses.In 8 samples (6,9%) FilmArray RP detected a virus while Pneumovir werenegative. In 8 samples (6,9%) Pneumovir detected a virus while FilmArrayRP were negative. One sample gave discrepant results.Conclusion: FilmArray Respiratory virus panel detected a co infectionin additional 12 samples compared to 2 for Pneumovir. Morestudies are needed to clarify the clinical implications of the viral coinfections.REF 532Enterovirus 68 circulation in hospitalized patients with respiratorysyndrome during 2010 2012 in Northern ItalyAntonio PIRALLA 1 , Alessia GIRELLO 1 , Michela GRIGNANI 2 ,Antonietta MARCHI 2 , Gianluigi MARSEGLIA 2 , Fausto BALDANTI 11 Molecular Virology Unit, Virology and Microbiology Department, FondazioneIRCCS Policlinico San Matteo, Pavia, ITALY; 2 Department ofPediatrics, Fondazione IRCCS Policlinico San Matteo, University of Pavia,Pavia, ITALYEnterovirus 68 (EV D68) was associated with mild to severe respiratoryinfections. In the last four years, circulation of different EV D68 strainshas been documented worldwide. Here, we describe the phylogenetic characterizationof nine EV D68 strains identified in hospitalized patients inthe 2010 2012 period and 12 additional EV D68 Italian strains previouslyidentified in 2008 in Northern Italy. From January 2010 to December 2012,a total of 889 respiratory from 588 patients hospitalized at the FondazioneIRCCS Policlinico San Matteo were positive for HRV or HEV. Extractednucleic acids were amplified by one step RT PCR with primer specific forVP1 region of EV D68 and purified positive PCR products were directlysequenced. Overall, 9/588 (1.53%) patients were EV D68 positive. Ofthese, 5/9 (77.7%) were pediatric and two (22.2%) were adults. Five outof seven (74.4%) pediatric patients had lower respiratory tract infectionwith oxygen saturation 90% of specimens, compared to 71% for GXF v2.0. False negativeswere observed in the low copy number range but not in core samples. Alsoin patients, overall concordance was good (>90%), with >75% of all discre-S268 Virologie, Vol 17, supplément 2, septembre 2013