rologie i - European Congress of Virology

5 th European Congress of Virologywith particular ethnic group and frequently used as a genetic marker forhuman migration in both prehistoric and modern times. The aims of thisstudy were to determine the frequency of JCV urinary shedding and genotypedistribution. Material and methods: Urine samples collected from107 healthy individuals were tested for presence of JCV DNA by PCR.A semi nested PCR was performed for amplification of 495 bp fragmentwitin VP1coding region and amplified fragments were directly sequenced.The genotypes of JCV isolates were determined by comparison to prototypesequences of the known genotypes in BioEdit software. Results: JCVDNA was detected in 31.7% of healthy individuals. Males had a higherexcretion rate than did the females (62% vs. 38%) and difference wasstatistically significant. In Serbian population genotype 1 was most prevalent41.2%, followed by genotype 4 in 31.4% and genotype 2 in 26.4%.A new variant of subtype 1A with a nucleotide substitution (C>G) at aposition 1940 was found in two samples. Conclusion:Considering geographicalposition and knowing that distribution of JCV genotypes mayreflect migration patterns, it is not surprising that the most prevalent genotypesin Serbia are 1 and 4 (European types) followed by genotype 2B, 2Cand 2D (Eurasian types).REF 503HIV 1 epidemic in Portuguese injecting drug users may be evolvinginto a unique molecular epidemiological patternJoão PIEDADE 1,2 , Carina SOUSA 1 , Sandra VIDEIRA E CASTRO 1 ,Elizabeth PÁDUA 3 , Ricardo PARREIRA 1,2 , Aida ESTEVES 1,21 Grupo de Virologia, Unidade de Microbiologia Médica, Instituto deHigiene e Medicina Tropical, Universidade Nova de Lisboa, Lisbon, POR-TUGAL; 2 Unidade de Parasitologia e Microbiologia Médicas (UPMM),Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa,Lisbon, PORTUGAL; 3 Lab. Nac. Referência IST VIH/SIDA e Hepatites Be C, Dep. de Doenças Infecciosas, Instituto Nacional de Saúde Dr. RicardoJorge, Lisbon, PORTUGALHIV 1 is characterised by a high degree of genetic diversity. HIV 1 geneticvariants are classified in 4 phylogenetic groups (M P) and group M issubdivided into 9 subtypes (A–D, F–H, J, K). Fast genetic recombinationgives rise to mosaic viruses, some of which gain epidemic proportions (circulatingrecombinant forms, CRF). According to their coreceptor (CCR5or CXCR4), HIV 1 variants can also be classified as R5, X4 or dual/mixedtropic. The aims of this study were to assess the genetic diversity of protease(PR), reverse transcriptase (RT), integrase (IN) and C2V3C3 codingsequences and to estimate the frequency of coreceptor usage in HIV 1strains circulating among 61 intravenous drug users from the Greater Lisbon.Viral RNA was amplified by RT nested PCR to originate PR, RT, INand C2V3C3 amplicons of 460, 650, 906 and 565 bp, respectively. 158DNA sequences were analysed, from 49 samples successfully amplifiedfor, at least, one of the regions. Subtype classification was achieved byphylogenetic analysis with MEGA4 and recombinant analysis was carriedout by bootscanning. A significant degree of HIV 1 diversity was shown.This epidemic is dominated by B and G subtypes, and their recombinantforms, but other genetic forms (F1, CRF02 AG) were also found. On thewhole, non B subtypes were identified in 58.9% (93/158) of the sequences.It is also significant that 45.2% (19/42) of the concatenated sequences studiedwere derived from inter genotype recombinants. Finally, coreceptorusage prediction classified 30 V3 amino acid sequences as R5 and 5 as X4or dual/mixed tropic.REF 504The value of real time sequence based information in surveillance ofhealthcare associated viral infectionsJ.C. RAHAMAT LANGENDOEN 1 , D.S. LUIJT 2 , A.D. PRENGER 3 ,M.WAGELAAR 3 ,A.OTT 2 , H.G.M. NIESTERS 11 Department of Medical Microbiology, Division of Clinical Virology, Universityof Groningen, University Medical Center Groningen, Groningen,THE NETHERLANDS; 2 Laboratory for Infectious Diseases, Groningen,THE NETHERLANDS; 3 Municipal Health Service, Groningen, THENETHERLANDSObjectives: sequence based information can serve as a tool to definetransmission routes. As most laboratories have not incorporated sequenceanalysis in their daily routine, information is mostly available retrospectively.Reducing the time to obtain sequence based information shouldbenefit the understanding of transmission and guide the implementationof appropriate infection control measures. Methods: in August 2012, realtime sequencing is introduced at the UMCG, a large tertiary referral hospital.A set of viruses, particularly noro, rhino, parecho and enterovirus, ischaracterized immediately after detection. To gain insight in viral diversityoutside the UMCG, a regional network is set up in which a regionallaboratory and the municipal health service submit samples for typing. Incase of an outbreak of gastroenteritis or respiratory illness in healthcareassociated institutions a limited set of clinical and epidemiological data iscollected.Results: sequence analysis results were available less than a week afterdetection. Several clusters of identical viruses were identified, especiallywith norovirus, confirming clonal transmission. Real time sequencing alsoenabled us to rapidly detect pseudo outbreaks where several norovirusgenotypes were found, providing evidence for multiple introductions ofdifferent strains rather than an ongoing transmission. Conclusion: realtime sequence analysis contributes to the understanding of transmissionof healthcare associated viral infections and enables us to focus infectioncontrol interventions adequately and timely.REF 505Frequency of distribution and variation of anal HPV genotypes andcorrelation with lifestyle and sexual behaviors among HIV infectedand non infected MSMs, compared to cervix samplesEszter UJHELYI, Csaba KOSA, Eszter SZABO, Edit BABARCZI, JanosSZLAVIK, Denes BANHEGYI, Istvan VALYI NAGYUnited Saint Istvan and Saint Laslo Hospital, Budapest, HUNGARYBackground: Anal cancer is one of the leading causes of death in nonAIDS defining cancers. Most of these cancers are associated with highrisk HPV (HR HPV) infection. No survey was made on anal HPV infectionin Hungarian MSM population before. We evaluated incidencesof cytological abnormalities and different genotypes, known and suspectedrisk factors, compared cervical and anal pattern. Materials andMethods: After obtaining informed concern, cervical and anal cytobrushwere taken and HPV genotyping with PCR (Roche Linear Array HPVgenotype).Every patient were inquired and tested about their sexual behavior,socioeconomic factors, drug use, and other known and suspected riskfactors. Risk assessments on this cross sectional cohort study were madeby Chi squared and odds ratio were calculated.S260 Virologie, Vol 17, supplément 2, septembre 2013