rologie i - European Congress of Virology

5 th European Congress of Virology(11, 3.5%) or partially discordant (52, 16.8%). While genotype specificagreement overall was very good (Kappa=0.903), HPV51 was significantlymore often detected by PG and the converse was true with HPV40,42, 54, and 68.Sixty five (77.4%) out of the 84 prospective samples were fully concordant(47 neg, 18 pos). Re evaluation of the 19 discordant cases after exchangingthe source of DNA was consistent with a higher sensitivity of the H28 assaydue mainly to the NIMBUS extraction procedure.H28 is a very sensitive and specific HPV assay for cervical cancerscreening as an adjunct to cytology and for epidemiological studies. Itsautomation capacity renders it particularly well suited for high workload.The additional benefit of its semi quantitative readout ought to be furtherevaluated for use as a tool in primary screening of cervical cancer.REF O172MxA: a diagnostic marker of viral infection in childrenIlka ENGELMANN 1 , Francois DUBOS 2 , Pierre Emmanuel LOBERT 1 ,Anne SARDET 3 , Anne DECOSTER 4 , Alain MARTINOT 2 , DidierHOBER 11 Université Lille 2, Faculté de Médecine, CHRU Lille, Laboratoire de virologieEA3610, Lille, France; 2 Université Lille Nord de France, UDSL,Faculté de Médecine, CHRU Lille, Urgences pédiatriques et maladiesinfectieuses, EA2694, Lille, France; 3 Service de Pédiatrie, Centre HospitalierDr Schaffner, Lens, FRANCE; 4 Laboratoire de l’Hôpital St Philibert,GHICL, Lomme, FRANCEObjective: The protein MxA is induced during viral infection and its bloodvalues could help to differentiate between viral and bacterial infection inchildren.Patients/Methods: We performed a prospective multicenter study includingchildren admitted at pediatric emergency departments. MxA bloodvalues were measured in children with confirmed viral infections, confirmedbacterial infections, uninfected controls and children with differentinfectious syndromes without microbiologic confirmation. The MxA thresholdindicative of viral infection and the diagnostic performance of MxAwere determined using ROC analysis.Results: MxA was measured in the blood of 556 children. The MxAthreshold for differentiating patients with viral infection from uninfectedpatients was determined using the first 44 uninfected controls and 77confirmed viral infections. The threshold was determined at 200 ng/mL.The other patients (n=435) formed the validation population. MxA levelswere higher in patients with viral infections than in those with bacterialinfections and uninfected controls (p