rologie i - European Congress of Virology

5 th European Congress of VirologyA total of 111 FFPE blocks from 79 skin lesions were collected from17 organ transplant recipients. HPV DNA detection was performed byPM PCR RHA. Viral infection was visualized by using a combinationof antibodies raised against the E4 and L1 proteins of the genotypes,alongside FISH to identify sites of viral genome amplification.Out of the 111 FFPE blocks analysed,94 were positive by PCR analysiswith universal primers. The commonest genotypes were HPV5 and8. All the tissue samples were probed for the viral markers. Six lesionsfrom 4 patients showed expression of cytoplasmic E4 overlapping withFISH positive nuclei and L1 in the more superficial layers.Positive areaswere detected in precancerous lesions or at the borders of more malignanttumours.Here,we demonstrate that HPV transcription is occurring at sites ofskin transformation in the OTR setting.Virus activation in sun exposedbody sites may cause UV exposures to be more deleterious to host cell,potentially increasing the likelihood that these cells become cancerous.Virologie, Vol 17, supplément 2, septembre 2013S103